Fabrication of self‐healing hydrogel from quaternized N‐[3(dimethylamino)propyl]methacrylamide copolymer for antimicrobial and drug release applications

Abstract

Self-healing hydrogels have attracted great attention in recent years because of their wide application in bioscience and biotechnology. In this study, P(DMAPMA-stat-DAA) were synthesized by Reversible Addition-Fragmentation Chain Transfer (RAFT) polymerization and quaternized to import antimicrobial properties. Then quaternized P(DMAPMA-stat-DAA) was used to prepare hydrogel containing acylhydrazone groups with Polyethylene oxide (PEO) diacylhydrazide as a cross-linking agent. The acylhydrazone groups imparted a variety of properties, including group responsiveness and self-healing properties to the hydrogel. At the same time, the quaternary ammonium endowed the hydrogel with the antimicrobial property. The mechanical property, self-healing properties, and antimicrobial property of hydrogels were investigated intensively. Results showed hydrogels formed in neutral conditions, and the luminescent property was introduced with PEO 23 dinaphthhydrazide (DNH) crosslinking. The hydrogels showed a controlled pH-sensitive DOX•HC l and Ovalbumin (OVA) release profile. In addition, the hydrogel showed the antimicrobial property and may have important applications in the biomedical field in the near future.

Hemostatic Self-Healing Hydrogel with Excellent Biocompatibility Composed of Polyphosphate-Conjugated Functional PNIPAM-Bearing Acylhydrazide

“…Mouse colon cancer cells (CT-26) were employed to investigate the cytotoxicity of the hydrogel solutions by CCK-8 assay . The RPMI 1640 (Solarbio, China) supplemented with 10% fetal bovine serum was used as the cell culture medium.…”

“…the hydrogel solutions by CCK-8 assay. 44 The RPMI 1640 (Solarbio, China) supplemented with 10% fetal bovine serum was used as the cell culture medium. The hydrogel solutions with various concentrations were prepared with growth medium as solvent.…”

Development of Hydrogels with Self-Healing Properties for Delivery of Bioactive Agents

“…As far as the production of self-healing hydrogels from synthetic polymers is concerned, acylhydrazone groups are one of the most extensively used functional groups owing partly to their ability to form stable gels under mild conditions . This has been demonstrated by an earlier study, in which reversible addition–fragmentation chain transfer (RAFT) polymerization for N -[3-(dimethylamino)­propyl]­methacrylamide (DMAPMA) and diacetone acrylamide (DAA) has been performed by using AIBN as an initiator and dodecyl- S ′-(α,α′-dimethyl-α′′-acetic acid)­trithiocarbonate (DDMAT) as a chain transfer agent . The product obtained has been quaternized and cross-linked to generate a self-healing hydrogel.…”

“…Upon injury, the hydrogel can heal the fracture via a continuous metathesis reaction. In addition, the presence of quaternary ammonium gives the hydrogel antimicrobial properties . Along with its pH-responsiveness which enables controlled bioactive agent release, the hydrogel shows the potential for further development as a self-healable carrier for anti-infection therapy.…”

“…This approach avoids the need to incorporate a healing agent into the hydrogel and ameliorates the safety concern over the toxicity caused by the release of the healing agent. Cross-links in self-healing hydrogels are generally noncovalent in nature (ranging from reversible ionic bonds and hydrogen bonds to hydrophobic or host–guest interactions), although the use of dynamic covalent bonds (involving imine groups, disulfide groups, and acylhydrazone groups) has also been reported (Figure ). − Over the years, different self-healing hydrogels have been developed for the delivery of not only bioactive agents but also living cells. − Examples of these are presented in Table .…”