Fabrication of size-controlled three-dimensional structures consisting of electrohydrodynamically produced polycaprolactone micro/nanofibers

Hong, Soongee; Kim, GeunHyung

doi:10.1007/s00339-011-6381-5

Cited by 45 publications

(19 citation statements)

References 23 publications

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“…Notably, the two layers showed stable integration without the delamination caused by the mechanical handling, which was probably due to the interadhesion between the random and aligned nanofiber layers generated by the remaining solvent of the electrospinning solution during the in situ deposition of random nanofibers over the aligned nanofiber layer. [ 18 ] The manual peel test utilizing the double‐sided tapes demonstrated the stable adhesion of the two layers, showing tearing off of the random nanofiber layer rather than the delamination of the aligned nanofiber layer, while the aligned nanofiber layer was almost selectively delaminated from the electrospun bilayer membrane fabricated by the conventional transfer method (Figure 1d and Figure S2, Supporting Information). Furthermore, the possible morphological structures of the interadhesion between the aligned and random nanofibers were also frequently observed in the highly magnified SEM images of the interface between the aligned and random nanofiber layers (Figure S3, Supporting Information).…”

Section: Resultsmentioning

confidence: 99%

Metal–Electrolyte Solution Dual‐Mode Electrospinning Process for In Situ Fabrication of Electrospun Bilayer Membrane

Han

Hong

Park

et al. 2020

Adv Materials Inter

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vivo extracellular matrix (ECM). [2] Various advantages of the electrospun nanofiber membranes, including broad material selection, a high surface-area-to-volume ratio, controllability of physical properties (e.g., diameter and orientation of the nanofibers and porosity and thickness of the membrane), and capability in fabrication of complex nanostructures (e.g., coreshell and Janus nanofibers), [3] have made remarkable advancements in the development of in vitro tissue models and in vivo tissue regeneration. [1,2] Many studies have tried to control the spatial orientation of the electrospun nanofibers through electrospinning, given that the cellular activities, such as adhesion, migration, proliferation, and differentiation, could be promoted by the contact guidance through an anisotropic topographical cue of the electrospun nanofibers. Among various approaches to control the orientation of the electrospun nanofibers, including the electric fieldassisted method, rotating drum method, and magnetic electrospinning, [4-6] the electric field-assisted method readily modulates the orientation of the electrospun nanofibers in a precise and versatile manner. Based on the electric field-assisted method, previous studies have achieved various types of membranes from a uniaxially aligned nanofiber membrane to radially, circumferentially, and biaxially aligned nanofiber membranes, [6-8] showing their potential in manipulating many cell functions, such as myogenic differentiation of myoblast, dural fibroblast migration, and tight junction formation of endothelial cells. [9-11] However, the aligned nanofiber membrane produced by the electric field-assisted method is mechanically unstable, in general, because of its thin (<30 µm thick), anisotropic, and sparsely interconnected nanofibrous structures. [12-14] Hence, the aligned nanofiber membrane is prone to be damaged by external forces occurred with the experimental and surgical manipulations and the aqueous conditions during in vitro and in vivo biomedical applications. [9,15,16] An innovative approach to overcome such limitations of the aligned nanofiber membranes is to develop an electrospun bilayer membrane, also known as a kind of Janus sheet, composed of two membranes, one aligned and the other random, which simultaneously provide the anisotropic topographical Electrospun bilayer membranes comprising two layers, one aligned and the other random, have shown great potential in tissue engineering but previous fabrication processes inevitably relied on manual integration and produced limited types of membranes. Here, a metal-electrolyte solution dual-mode electrospinning (M-ELES) for fabrication of electrospun bilayer membrane based on a metal-electrolyte solution switchable collector is developed. The switchable collector enables random nanofiber deposition directly over the preexisting aligned nanofiber layer in an in situ manner and integration of the layers through an on-demand switch from the metal to the electrolyte solution collector. The electrolyte solution can eff...

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Section: Resultsmentioning

confidence: 99%

Metal–Electrolyte Solution Dual‐Mode Electrospinning Process for In Situ Fabrication of Electrospun Bilayer Membrane

Han

Hong

Park

et al. 2020

Adv Materials Inter

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“…Wet electrospinning is one of the most widely used methods for preparing 3D nanofibrous scaffolds, which refers to the collection of electrospun fibers by replacing the traditional collecting plates with a liquid bath as dispersant and coagulant (Figure b). Methanol (Holmes, Castro, Li, Keidar, & Zhang, ; Ki et al, ; Ki et al, ; Shin, Park, Kim, Lee, & Youk, ), and ethanol (Hong & Kim, ) are commonly used as liquid bath due to their low surface energy and availability. A high‐speed rotating stirrer is used to evenly distribute the fibers in the liquid bath.…”

Section: Fabrication Of 3d Nanofibrous Scaffoldsmentioning

confidence: 99%

“…A high‐speed rotating stirrer is used to evenly distribute the fibers in the liquid bath. In addition, after collecting the 3D nanofibers, a freeze‐drying process is always applied to retain the 3D shape (Hong & Kim, ).…”

Section: Fabrication Of 3d Nanofibrous Scaffoldsmentioning

confidence: 99%

Three‐dimensional electrospun nanofibrous scaffolds for bone tissue engineering

et al. 2019

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Electrospinning technology has been widely used in the past few decades to prepare nanofibrous scaffolds that mimic extracellular matrices. However, traditional two‐dimensional (2D) electrospun nanofibrous mats still have some inherent disadvantages for bone tissue engineering, such as limited cell infiltration and lack of three‐dimensional (3D) structure. The development of 3D electrospun scaffolds with larger pore sizes and porosity provides new perspectives for electrospinning‐based tissue engineering scaffolds. However, there are still some challenges and areas for improvement. In this review, the applications of 3D electrospun nanofibrous scaffolds in the field of bone tissue engineering from its fabrication methods to bio‐functionalization are summarized, with the aim of providing new insights into the design of electrospinning‐based bone tissue engineering scaffolds.

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“…Highly porous, 3D scaffolds can be obtained via an alternative electrospinning approach: using a coagulation bath of non-solvent as the collector. [22][23][24][25][26][27][28][29][30][31][32][33][34][35] However, while these studies have yielded 3D scaffolds composed of fibers that are not straight, the random fiber morphology is very far from the desired coiled, hierarchal morphology. Recently, work by Taskin et al 36 described electrospinning of polycaprolactone (PCL) into an ethanol coagulation bath to obtain highly porous 3D scaffolds composed of coiled fibers, which facilitated myofibroblast differentiation and contraction.…”

Section: Introductionmentioning

confidence: 99%

Architectured helically coiled scaffolds from elastomeric poly(butylene succinate) (PBS) copolyester via wet electrospinning

Sonseca¹,

Sahay²,

Stępień³

et al. 2019

Preprint

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<div><div><div><p>Electrospinning is one of the most investigated methods used to produce polymeric fiber structures that mimic the morphology of native extracellular matrix. These structures have been extensively studied in the context of scaffolds for tissue regeneration. However, the compactness of materials obtained by traditional electrospinning, collected as two-dimensional non-woven scaffolds, can limit cell infiltration and tissue ingrowth. In addition, for applications in smooth muscle tissue engineering, highly elastic scaffolds capable of withstanding cyclic mechanical strains without suffering significant permanent deformations are preferred. In order to address these challenges, we report the fabrication of microscale 3D helically coiled structures (referred as 3D-HCS) by wet-electrospinning method, a modification of the traditional electrospinning process in which a coagulation bath (non-solvent system for the electrospun material) is used as the collector. The present study, for the first time, successfully demonstrates the feasibility of using this method to produce various architectures of 3D-HCS from segmented copolyester of poly(butylene succinate-co- dilinoleic succinate) (PBS-DLS), a thermoplastic elastomer. A mechanism for the HCS formation is proposed and verified with experimental data. Fabricated 3D-HCS showed high specific surface area, high porosity, and good elasticity. Further, the marked increase in cell proliferation on 3D-HCS confirmed the suitability of these materials as scaffolds for soft tissue engineering.</p></div></div></div>

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Fabrication of size-controlled three-dimensional structures consisting of electrohydrodynamically produced polycaprolactone micro/nanofibers

Cited by 45 publications

References 23 publications

Metal–Electrolyte Solution Dual‐Mode Electrospinning Process for In Situ Fabrication of Electrospun Bilayer Membrane

Metal–Electrolyte Solution Dual‐Mode Electrospinning Process for In Situ Fabrication of Electrospun Bilayer Membrane

Three‐dimensional electrospun nanofibrous scaffolds for bone tissue engineering

Architectured helically coiled scaffolds from elastomeric poly(butylene succinate) (PBS) copolyester via wet electrospinning

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