Fabrication of Solid Collagen Nanoparticles Using Electrospray Deposition

Abstract

Collagen is a promising biomaterial for drug delivery due to advantages including high biocompatibility and biodegradable property. However, transforming collagen into solid nanoparticles is difficult, although the solid dosage form is advantageous for some administration routes including pulmonary and oral drug delivery. In this study, collagen solid nanoparticles are prepared in one-step using electrospray deposition under ambient temperature and pressure conditions. Although collagen molecules formed micron-sized aggregates in acetic acid solutions spontaneously, electrospraying the collagen solutions resulted in formation of nanofibers. Solid nanoparticles were obtained by increasing conductivity of the solution and/or inducing structural perturbation of the collagen molecules using salts. The ability of solid collagen particles as a drug carrier was demonstrated by incorporating theophylline as a model drug using a coaxial spray technique. Release of theophylline was controlled by cross-linking collagen molecules. Electrospray deposition was proved to be a powerful method for producing solid collagen nanoparticles for drug delivery.

Protein Polymer-Based Nanoparticles: Fabrication and Medical Applications

“…Due to collagen’s biocompatibility and low antigenicity, collagen-based nanoparticles have been used for the delivery of pharmaceuticals such as theophylline, retinol, tretinoin, and lidocaine [ 13 , 50 , 51 ]. Collagen is capable of resembling the microenvironment of some tumors allowing collagen nanoparticles to effectively infiltrate the areas and deliver anticancer therapeutics [ 52 ].…”

In situ Enabling Approaches for Tissue Regeneration: Current Challenges and New Developments

“…Several types of natural polymers, such as chitosan (Songsurang et al, 2011), alginate (Park et al, 2012), cellulose (Huang et al, 2012), or collagen (Nagarajan et al, 2014), as well as synthetic PLGA (Nath et al, 2013), PCL (Hwang et al, 2008), and polystyrene (PS) (Zheng et al, 2006) have been successfully electrosprayed to generate nano/micro-particulate systems.…”

Nano drug delivery systems in upper gastrointestinal cancer therapy

“…Animal studies on DOX-chitosan. Clinical studies (mainly lung and breast cancer): chitosan NPs loaded with curcumin, cisplatin or ascorbate [ 77 , 176 ] Collagen Conjugation with metal NPs, high biocompatibility, suitable for inhalation, controlled release Delivery of collagenase desireable in cancer—not possible with collagen, difficult particle fabrication Research: silver NP stabilized with collagen [ 177 , 178 ] Alginate Mucoadhesive, pH sensitive, oral delivery, suitable for micelles, stabilizer for metal NPs Unknown toxicity, need stabilizers Preclinical studies: magnetic chitosan/alginate- curcumin NP, exemestane-ALG-NPs, paclitaxel-loaded ALG-NP [ 155 , 156 ] Cellulose pH triggered release, completely biodegradable, oral administration, increase drug solubility Difficult fabrication, aggregation due to hydrophylic nature, no release in acidic pH (GC), insufficient knowledge about interaction with cells and tissues Approved: carboxymethyl cellulose. Clinical Trial: eethylcellulose + cetuximab [ 76 , 179 , 180 ] Metal Se Antioxidant, anti-inflammatory properties, anticancer activity, dual delivery of therapeutics like siRNA + cisplatin, curcumin SeNPs Dual role in cancerogenesis and drug delivery—might also produce radicals, possible epigenetical modifications Only in vitro cell studies [ 167 , 181 , 182 ] Au High surface to volume ratio, stable, ideal plasmon resonance for therapeutic applications like photothermal and photodynamic therapy, good biosafety profile, high permeability, scalable Limitations regarding bioavailability of drugs, possible cytotoxicity depending on shape and size, changes in gene expression Approval: DOX-AuNP, clinical trial: oxaliplatin-platinium-AuNP [ 183 ] …”