Facile and green synthesis of pullulan derivative-stabilized Au nanoparticles as drug carriers for enhancing anticancer activity

Methods for Reducing the Toxicity of Metal and Metal Oxide NPs as Biomedicine

“…An example of obtaining metallic nanoparticles by the biological method is the synthesis of nano gold modified with para-aminobenzoic acid-quat-pullulan (PABA-QP) as a carrier of doxorubicin. Due to surface modification, higher drug loading was possible, which resulted in a cytotoxicity 2.1-times higher against Chago cells compared to pure doxorubicin [52].…”

“…However, the biopolymers used have some disadvantages, such as low solubility and high viscosity. Laksee et al used pullulan as a nonionic polysaccharide that showed high water solubility, non-toxicity, and biocompatibility while maintaining a low price [52]. Therefore, the main challenge is to limit the toxic effects of pure Au NPs as they have high surface binding energy with the surrounding environment, which can cause protein adsorption.…”

“…Due to surface-modified Au NPs, doxorubicin was released in cancer cells. Au NPs protected the drug outside the cancer cells without causing the drug to enter healthy cells [52]. Woźniak et al examined the cytotoxicity of Au NPs in terms of the coating agent used.…”

Nanoengineering of Gold Nanoparticles: Green Synthesis, Characterization, and Applications

“…The same group also utilized modified para-aminobenzoic acid-quat188-pullulan (PABA-QP) for the synthesis of AuNPs. These NPs were used as nanocarriers of DOX, revealing greater toxicity against cancer cells and, simultaneously, less toxicity against normal cells compared with free DOX [188]. In addition, AuNPs stabilized with Pullulan were functionalized with 5FU and folic acid for their potential use in drug administration.…”

Desenvolvimento e avaliação de nova preparação de anfotericina B e/ou cetoconazol em dispersão de bixina, pullulan e trealose

“…Uma recente aplicação do pullulan tem sido na química verde, na produção de nanopartículas. O uso de pullulan puro ou de pullulan modificado com grupos funcionais se mostrou promissor como agente redutor, estabilizante e de revestimento para a produção de nanopartículas sólidas (LAKSEE et al, 2018;GANESHKUMAR et al, 2014;COSERI et al, 2015, LAKSEE et al, 2017NEOSUWAN et al, 2018;KRULL et al, 2016; KANAMANI e LIM, 2013). Alguns desses sistemas nanoparticulados foram capazes de carrear fármacos e aprimorar o efeito dos mesmos (LAKSEE et al, 2018;GANESHKUMAR et al, 2014;LAKSEE et al, 2017;NEOSUWAN et al, 2018).…”