Facile and inexpensive fabrication of zinc oxide based bio-surfaces for C-reactive protein detection

Cao, Lu; Kiely, Janice; Piano, Martina; Luxton, Richard

doi:10.1038/s41598-018-30793-z

Cited by 31 publications

(25 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The sensor response was plotted using readings taken at a frequency of 138 Hz. This frequency was selected as in previous work it demonstrated maximum changes in response to addition of a target analyte [15]. This observation concurred with Jacobs et al, who found that the electrical double layer is greater at frequencies below 1000 Hz and the most significant changes occurred around 100 Hz in their experiment to measure the protein troponin-T on a ZnO surface [16].…”

Section: Methodssupporting

confidence: 74%

See 1 more Smart Citation

A Copper Oxide/Zinc Oxide Composite Nano-Surface for Use in a Biosensor

et al. 2019

Self Cite

View full text Add to dashboard Cite

In this study, biosensors based on zinc oxide–copper oxide composite nano-surfaces were prepared using a simple and inexpensive distributed colloidal technique. Combinations of mixed dispersions with volume ratios of 1:1, 1:2 and 2:1 ZnO:CuO were compared. The uniform nano-crystalline sensor surfaces on polyethylene terephthalate (PET) were analysed using scanning electron microscopy (SEM), Atomic Force Microscopy (AFM) and Raman Spectroscopy. The ZnO–CuO composite biosensor nano-surfaces showed a significantly increased impedimetric signal compared with pure ZnO nanocrystals, and the maximum output was achieved with a volume ratio of 1:2 ZnO/CuO. The antibody capture of C-reactive protein (CRP) on the nano-surfaces was used to demonstrate the enhanced signal generated with increasing amounts of CuO in the nano-surface.

show abstract

Section: Methodssupporting

confidence: 74%

“…200 μL of each suspension were dropped on to clean polyethylene terephthalate (PET) substrates (20 mm × 20 mm) separately to make ZnO/CuO nano-surfaces. Finally, they were dried in an oven at 65 ∘C for 80 min and cooled down to room temperature, then stored in a dry atmosphere with silica gel for up to 2 days [15].…”

Section: Methodsmentioning

confidence: 99%

A Copper Oxide/Zinc Oxide Composite Nano-Surface for Use in a Biosensor

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…In fact, Wang et al have concluded that ZnO NRs provided more protein interactions than other ZnO nanostructured materials 37 , reinforcing the development of ZnO NR-based immune-dependent platforms. For instance, Cao and colleagues have developed ZnO NRs-based sensors to immunodetect C-reactive protein, where a colloidal dispersion technique was used to cover PET 4,38 . Similarly, Tamashevski et al 15 developed a ZnO NRs-based sensor towards the immunodetection of human leukemic cells, where gaseous-dispersive synthesis was used to cover a glass substrate.…”

Section: Resultsmentioning

confidence: 99%

“…9.5, makes it suitable to adsorb negatively charged proteins, such as enzymes and antibodies, with lower IEPs 3 . As such, various types of biomolecules, including proteins 4 , enzymes 5 , antibodies 6 , as well as nucleic acids 7 , have been successfully bound to ZnO nanorods.…”

Section: Introductionmentioning

confidence: 99%

Flexible ZnO-mAb nanoplatforms for selective peripheral blood mononuclear cell immobilization

Babu

Pinheiro

Marques

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Cancer is the second cause of death worldwide. This devastating disease requires specific, fast, and affordable solutions to mitigate and reverse this trend. A step towards cancer-fighting lies in the isolation of natural killer (NK) cells, a set of innate immune cells, that can either be used as biomarkers of tumorigenesis or, after autologous transplantation, to fight aggressive metastatic cells. In order to specifically isolate NK cells (which express the surface NKp30 receptor) from peripheral blood mononuclear cells, a ZnO immunoaffinity-based platform was developed by electrodeposition of the metal oxide on a flexible indium tin oxide (ITO)-coated polyethylene terephthalate (PET) substrate. The resulting crystalline and well-aligned ZnO nanorods (NRs) proved their efficiency in immobilizing monoclonal anti-human NKp30 antibodies (mAb), obviating the need for additional procedures for mAb immobilization. The presence of NK cells on the peripheral blood mononuclear cell (PBMCs) fraction was evaluated by the response to their natural ligand (B7-H6) using an acridine orange (AO)-based assay. The successful selection of NK cells from PBMCs by our nanoplatform was assessed by the photoluminescent properties of AO. This easy and straightforward ZnO-mAb nanoplatform paves the way for the design of biosensors for clinic diagnosis, and, due to its inherent biocompatibility, for the initial selection of NK cells for autotransplantation immunotherapies.

show abstract

“…This study was conducted using anti-FIX as bioreceptor, while FIX as the target for this detection and the detection limit was 100 pM. While the specific attention was narrowed down by implementing thin film technology to design novel nanosensors 6 . Thin film materials are unique due to their nano-or micro-dimensions nature followed by a superior electrical conductivity for quick and accurate outcome, which would easier for early detection system in the medical field 7 .…”

mentioning

confidence: 99%

Comparative Analysis on Dielectric Gold and Aluminium Triangular Junctions: Impact of Ionic Strength and Background Electrolyte by pH Variations

Letchumanan

Arshad

Gopinath

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Field of generating a surface thin film is emerging broadly in sensing applications to obtain the quick and fast results by forming the high-performance sensors. Incorporation of thin film technologies in sensor development for the better sensing could be a promising way to attain the current requirements. This work predominantly delineates the fabrication of the dielectric sensor using two different sensing materials (Gold and Aluminium). conventional photolithography was carried out using silicon as a base material and the photo mask of the dielectric sensor was designed by AutocAD software. the physical characterization of the fabricated sensor was done by Scanning electron Microscope, Atomic Force Microscope, High Power Microscope and 3D-nano profiler. The electrical characterization was performed using Keithley 6487 picoammeter with a linear sweep voltage of 0 to 2 V at 0.01 V step voltage. By pH scouting, i-V measurements on the bare sensor were carried out, whereby the gold electrodes conducts a least current than aluminium dielectrodes. comparative analysis with pH scouting reveals that gold electrode is suitable under varied ionic strengths and background electrolytes, whereas aluminium electrodes were affected by the extreme acid (pH 1) and alkali (pH 12) solutions.

show abstract

Facile and inexpensive fabrication of zinc oxide based bio-surfaces for C-reactive protein detection

Cited by 31 publications

References 39 publications

A Copper Oxide/Zinc Oxide Composite Nano-Surface for Use in a Biosensor

A Copper Oxide/Zinc Oxide Composite Nano-Surface for Use in a Biosensor

Flexible ZnO-mAb nanoplatforms for selective peripheral blood mononuclear cell immobilization

Comparative Analysis on Dielectric Gold and Aluminium Triangular Junctions: Impact of Ionic Strength and Background Electrolyte by pH Variations

Contact Info

Product

Resources

About