Facile and sensitive detection of dopamine based on in situ formation of fluorescent polydopamine nanoparticles catalyzed by peroxidase-like ficin

Pang, Yanjiao; Shi, Ying; Pan, Yadi; Yang, Yating; Long, Yijuan; Zheng, Huzhi

doi:10.1016/j.snb.2018.02.128

Cited by 33 publications

(12 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The selectivity study was further extended for H 2 O 2 and DA in the presence of reactive oxygen radicals, generated in situ in the dispersion using Fe 3 O 4 +H 2 O 2 . The results showed that H 2 O 2 by itself did not produce quenching of PL emission, whereas the DA in presence of oxygen radicals led to significant quenching of PL of nanoplatform, indicating that oxygen radicals facilitated the rapid oxidation of DA to q‐DA, consistent with the previous report,, which was detected by the probe; thus, corroborating that the oxidation of DA to q‐DA is an indispensable event behind the DA detection process by the nanoprobe.…”

Section: Resultssupporting

confidence: 87%

Upconversion Nanoplatform for FRET‐Based Sensing of Dopamine and pH

2019

View full text Add to dashboard Cite

Dopamine (DA) plays a very critical role in controlling the key functionalities, such as in metabolic, memory and behavioural aspects. The deviation from the normal range of concentration has been linked to serious neurological disorders and cancers. Therefore, development of a facile sensing platform with a prospective potential to be applied in the real‐time analysis of DA in vivo holds a great promise. To achieve this, an upconversion nanoparticle (UCNP) based nanoplatform with mesoporous silica functionality (BSCU‐MS) has been proposed as an efficient DA sensing probe. The UCNP has been tuned to exhibit emission in particular region with high PL intensity that could be selectively absorbed by the DA in quinone form (q‐DA) through Förster resonance energy transfer (FRET) mechanism. We showed that the proposed nanoplatforms are capable of detecting DA in a sensitive and very selective manner with the limit of detection (LOD) as 0.63 nM (with S/N=3). Also, owing to the interesting electronic behaviour of DA to undergo pH‐dependent oxidation and reduction process, we also demonstrate the applicability of DA loaded BCSU‐MS for fluorescence‐based pH sensing. We propose that the method described offers a very promising and efficient strategy for the detection of DA‐related diseases and the ensuing physiological pH changes.

show abstract

Section: Resultssupporting

confidence: 87%

Upconversion Nanoplatform for FRET‐Based Sensing of Dopamine and pH

2019

View full text Add to dashboard Cite

show abstract

“…This way, the enzyme can transform peroxidase substrates to colored products in the presence of hydrogen peroxide, being the active site of the protease activity different to that of the peroxidase activity. Ficin peroxidase activity was utilized to in situ synthesize intrinsic fluorescent polydopamine nanoparticles and to develop a rapid dopamine sensing method [152].…”

Section: -Ficin Promiscuous Activities: Enlarging the Range Of Ficin Application Out Of Proteases Rangementioning

confidence: 99%

Ficin: A protease extract with relevance in biotechnology and biocatalysis

Morellon‐Sterling

El-Siar

Tavano

et al. 2020

International Journal of Biological Macromolecules

View full text Add to dashboard Cite

This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

show abstract

“…For instance, MPDA NPs conjugated with fluorophore‐labeled DNA probe cytosensors were developed for the sensitive detection of microRNA (miRNA) (biomarkers for cancer diagnosis) based on the fluorescence quenching principle . Furthermore, fluorescent PDA (FPDA) NPs fabricated by a facile approach based on a peroxidase‐catalytic enzyme (ficin) were reported for the detection of DA in various DA‐related diseases . Briefly, DA sensing is based on measuring the fluorescence signal of PDA NPs, which could be in situ formed by catalyzing the oxidation of DA in the presence of H 2 O 2 , produced as a result of the peroxidase‐like activity of ficin.…”

Section: Colloidal Pda Particlesmentioning

confidence: 99%

Current Advances in the Utilization of Polydopamine Nanostructures in Biomedical Therapy

Tran

Batul

Bhave

et al. 2019

Biotechnology Journal

View full text Add to dashboard Cite

Since the first time mussel‐inspired polymer polydopamine (PDA) was discovered, it has gained enormous attention from numerous scientists, especially those working in the field of drug delivery and bacterial and tumor treatment, due to its distinctive properties, such as surface chemistry, biocompatibility, capability to adhere to any surface, and excellent photothermal conversion. Studies using PDA in various types of structures for therapeutic purposes have been carried out extensively in recent years. Considering the rapid development in the area, this review aims to cover and highlight the latest achievements (from 2016 to present) with respect to PDA‐based materials for therapeutic purposes. A description of the diverse structures of PDA and its formation strategy, including colloidal particles, hollow structures, and coating films, are discussed. In addition, the main focus of this review is on the therapeutic applications of these PDA nanostructures.

show abstract

Facile and sensitive detection of dopamine based on in situ formation of fluorescent polydopamine nanoparticles catalyzed by peroxidase-like ficin

Cited by 33 publications

References 38 publications

Upconversion Nanoplatform for FRET‐Based Sensing of Dopamine and pH

Upconversion Nanoplatform for FRET‐Based Sensing of Dopamine and pH

Ficin: A protease extract with relevance in biotechnology and biocatalysis

Current Advances in the Utilization of Polydopamine Nanostructures in Biomedical Therapy

Contact Info

Product

Resources

About