2015
DOI: 10.1016/j.snb.2014.12.073
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Facile electrochemical detection of Escherichia coli using redox cycling of the product generated by the intracellular β-d-galactosidase

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Cited by 20 publications
(7 citation statements)
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“…The electrochemical detection of 4aminonaphthol amplified by an electrochemical-chemical redox cycling led to a detection limit of 10 5 CFU/mL for 30 min of IPTG treatment. By this method, a detection limit of 1.0 CFU/mL was reached with a 4-hour IPTG treatment, without DNA amplification or immunoassay protocol [37].…”
Section: ) Amperometric Biosensorsmentioning
confidence: 99%
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“…The electrochemical detection of 4aminonaphthol amplified by an electrochemical-chemical redox cycling led to a detection limit of 10 5 CFU/mL for 30 min of IPTG treatment. By this method, a detection limit of 1.0 CFU/mL was reached with a 4-hour IPTG treatment, without DNA amplification or immunoassay protocol [37].…”
Section: ) Amperometric Biosensorsmentioning
confidence: 99%
“…In another study, a method for easy detection of E. coli has been established based on the enzymatic activity of intracellular -D-galactosidase (Gal) [36]. The bacteria was first treated with a gene expression inducer (isopropyl--Dthiogalactopyranoside IPTG), leading to the increase of the expression level of Gal inside E. coli.…”
Section: ) Amperometric Biosensorsmentioning
confidence: 99%
“…The proposed system is comparable with or even better than other enzyme-based assays. [33][34][35][36][37][38] More importantly, peptide functionalized magnetic beads offer the benefits of simplicity, speed, stable and flexibility to perform potentiometric detection based on surface charge change. The detection time is shorter than most commercially available detection kits for E. coli.…”
Section: Potentiometric Detection Of E Colimentioning
confidence: 99%
“…17b. In this type of biosensor, antigens are specifically bound to the sensing surface to ensure the close proximity of the signaling 100,101 Moreover, the modification of the ITO surface by reduced grapheme oxide could further improve the biosensing performance for BoNT/E-LC. 102 Thus, the discussion of this section clearly justifies the excellent biosensing performance achieved by redox cycling, and when the antigen itself is electroactive, then it can produce a redox-cycling-based signal amplification (following Fig.…”
Section: +mentioning
confidence: 99%