Facile Fabrication of Flowerlike Natural Nanotube/Layered Double Hydroxide Composites as Effective Carrier for Lysozyme Immobilization

Abstract

Carrier-based immobilization has been developed to enhance enzymatic stability and activity, which permits the employment of enzymes in different solvents, at wide ranges of pH and temperature as well as high substrate concentrations. In this study, a novel carrier was prepared with halloysite nanotubes (HNTs) and layered double hydroxide (LDH) via a layer-by-layer (LbL) deposition process followed by an in situ growth technique. The in situ growth of LDH nanoplatelets on a HNTs support was demonstrated producing a well-defined three-dimensional architecture (HNTs@LDH). These flowerlike structural materials possess a high lysozyme immobilized amount (237.6 mg/g support) compared with individual HNTs and LDH. And such lysozyme immobilized composites (HNT@LDH−Ly) exhibit a superior antibacterial property against Escherichia coli (E. coli).

Halloysite Clay Nanotubes for Enzyme Immobilization

“…In some publications on enzyme immobilization on halloysite nanotubes, it was demonstrated that having additional intermediate molecules that bonds to halloysite on one end and to the enzyme on the other gives functional biocomposites. These include peroxidase and laccase immobilized to halloysite micelles with dopamine or lysozyme immobilized on halloysite–double hydroxide composites. − , These three- or four-component composites are complicated, but allowed, for higher enzymatic activity and may be practically useful; however, the location of the enzymes was unclear, and the relationship between the sorption process and its effects on the enzymes was not fully developed. In these works, halloysite lacked detailed analysis on the interaction between the clay nanotubes and enzyme.…”

PdNP Decoration of Halloysite Lumen via Selective Grafting of Ionic Liquid onto the Aluminol Surfaces and Catalytic Application

“…Because of its tubular structure and robustness, halloysite was used as nanoparticles support, − as nanocontainer for drug release, − as corrosion inhibitor carrier, , and as nanoreactor for enzymatic reaction …”

Flower-like Surface of Three-Metal-Component Layered Double Hydroxide Composites for Improved Antibacterial Activity of Lysozyme

“…The high surface area of nano/micromaterials can absorb more lysozyme and induce the enhancement of antibacterial activity. 38,39 Moreover, the different sizes of nanomaterials can influence the antibacterial activity. Few studies in the literature have reported the morphological antibacterial effect.…”

“…Some work has been published to enhance the antibacterial activity of lysozyme with the aid of nano/microsize materials. The high surface area of nano/micromaterials can absorb more lysozyme and induce the enhancement of antibacterial activity. , Moreover, the different sizes of nanomaterials can influence the antibacterial activity. Few studies in the literature have reported the morphological antibacterial effect. , The morphology of LDH can be tuned by changing the components and ratio of cations.…”