Facile in Situ Synthesis of Silver Nanoparticles on Procyanidin-Grafted Eggshell Membrane and Their Catalytic Properties

Abstract

Facile, efficient, and robust immobilization of metal nanostructures on porous bioscaffolds is an interesting topic in materials chemistry and heterogeneous catalysis. This study reports a facile in situ method for the synthesis and immobilization of small silver nanoparticles (AgNPs) at room temperature on natural eggshell membrane (ESM), which presents interwoven fibrous structure and can be used as a unique protein-based biotemplate. Procyanidin (Pro), a typical plant polyphenol extracted from grape seeds and skins, was first grafted onto ESM fibers to serve as both reductant and stabilizer during the synthesis process. As a result, the AgNPs were facilely synthesized and robustly immobilized on the ESM fibers without additional chemical reductant or physical treatments. The morphology and microstructure of the as-prepared AgNPs@Pro-ESM composites were characterized by combined microscopy and spectroscopy technologies. The results indicate that small AgNPs with mean diameter of 2.46 nm were successfully prepared on the Pro-ESM biotemplate. The composites exhibited good catalytic activity toward the reduction of 4-nitrophenol (4-NP). More importantly, these composite catalysts can be easily recovered and reused for more than eight cycles because of their high stability.

Silver Nanoprism-Loaded Eggshell Membrane: A Facile Platform for In Situ SERS Monitoring of Catalytic Reactions

“…In a high-resolution XPS spectrum ( Figure 4B), two peaks were observed at 367.9 and 373.9 eV which are attributed to Ag 3d 5/2 and Ag 3d 3/2 of elemental silver. The splitting of the 3d doublet of Ag is 6.0 eV, indicating the presence of metallic Ag, which provides solid evidence for the assembly of AgNPs on the fiber surface [27].…”

“…The intensity of the absorption peak at 400 nm decreased dramatically with reaction time, indicating the decrease of the initial reactant (4-NP). Meanwhile, a new absorption peak at approximately 300 nm appeared during this process, suggesting the generation of reduction product 4-AP [27,40]. Figure 5C displays the plot of the absorption intensity at 400 nm as a function of time corresponding to the treated ESMs.…”

“…Many supports including filter paper [23], cellulosic fibers [24], and polymer hydrogel [25] for the immobilization of nanoparticles have been already explored. Recently, natural biomass materials such as eggshell membrane [26][27][28], plant tissue [29] and mushroom [30] as effective catalyst supports provide "green" platforms to the catalytic reaction. Generally, ESMs, together with eggshells, are disposed of as waste materials.…”

“…Recently, ESMs have been widely investigated because they are a rich resource with fascinating structures. Utilizations based on ESMs have been developed in various fields, such as heavy metal ions adsorption [31], high-performance electrode materials [32], as a separator in supercapacitor [33,34], biosensing [35] and catalysis [26][27][28].…”

Optimization and integration of nanosilver on polycaprolactone nanofibrous mesh for bacterial inhibition and wound healing in vitro and in vivo

“…13 The peak became sharper in the PCL/NS0.5, PCL/NS1.0 and PCL/NS2.0 spectra, suggesting the interaction between NS and DA. 40 In addition, the stronger peaks at 1,166 cm -1 , 1,726 cm -1 and 2,930 cm -1 also indicated the deposition of NS on the PCL nanofibers.…”