Facile Preparation of Hemin-functionalized Electrochemically Reduced Graphene Oxide Nanocomposite for H2O2 Biosensing

Abstract

A Hemin-f unctionalized electrochemically reduced graphene oxide (H-ERGO) nanocomposite was prepared for the construction of a nonenzymatic H 2 O 2 biosensor. H-ERGO was directly modified on the electrode surface without using a binder by electrochemical reduction. Detailed physical and electrical characterizations of H-ERGO were conducted by scanning electron microscopy, UV-vis absorption spectroscopy, and electrochemical impedance spectroscopy (EIS). Taking advantage of the superior catalytic properties of H-ERGO, we applied the proposed biosensor to H 2 O 2 analysis. Under optimized conditions, the H-ERGObased biosensor exhibited a wide linear range from 25 to 8850 and 8850 to 28850 μM, with a detection limit (S/N = 3) of 8.33 μM for H 2 O 2 determination. Furthermore, it was also successfully applied to detect H 2 O 2 in milk samples with recoveries ranging from 94.6 to 98.1%.

Electrochemical Sensor for the Detection of 1-Hydroxypyrene Based on Composites of PAMAM-Regulated Chromium-Centered Metal–Organic Framework Nanoparticles and Graphene Oxide

“…This problem can be solved by introducing other materials with good conductivity and electrocatalytic performance such as carbon nanomaterials, , metal nanoparticles, , conducting polymers, , and so forth, which gives them both the advantages of MOFs and good electrical conductivity; additionally, the synergistic effects of MOF composites would further broaden the application in the field of electrochemical sensors. As a representative materials of carbon nanomaterials, graphene oxide (GO) has been widely used in the synthesis of MOF nanocomposites due to its abundant oxygen-containing functional groups, large specific surface area, good electrical conductivity, robust mechanical properties, and other inherent advantageous properties. − The introduction of GO can not only enhance the adsorption capacity of the target by π–π stacking, hydrogen bonding, and other forces but also effectively improve the conductivity of MOF nanocomposites, which could result in a synergistic effect to improve the electrochemical active area and sensitivity of the electrochemical sensing. , In addition, the addition of GO possessing excellent stability in aqueous solutions could greatly improve the poor stability of MOF nanocomposites and dispersion degree in water so as to significantly enhance electrochemical and electrocatalytic performances. , Because graphene’s rapid electron transport occurs on the surface of edge plane and defects, GO has been reduced to defective reduced GO (RGO) by various methods to improve the electrocatalytic properties of graphene-MOF composites, such as chemically reduced, electrochemically reduced, and so on . Additionally, the morphology and pore size of the nanocomposite were improved by introducing polymer surfactants or chemical modification and hybridization of the MOF in an in situ assembly. , The result is that the electrochemical performance of MOF-based composite materials is enhanced more than that of single-component MOFs due to the combination of the merits of both MOFs and other materials.…”

Durable Hydrogen Peroxide Biosensors Based on Polypyrrole-Decorated Platinum/Palladium Bimetallic Nanoparticles

“…The changed current was 67.9 μA for 0.4 mM H 2 O 2 , and the sensitivity of the proposed sensor was 1360.83 μA·mM –1 ·cm –2 . In this study, the signal sensitivity of the H 2 O 2 sensor was relatively higher than those of previous studies, in which the signal sensitivities of H 2 O 2 sensors were from 687.3 to 859.87 μA·mM –1 ·cm –2 . , Based on the signal-to-noise threshold (>3), the limit of detection (LOD) of the present method was set to be 2.5 μM, which is at the medium level of previous reports on H 2 O 2 biosensors. ,, …”

“…In addition, considering the biocompatibility of polypyrrole and Pt and Pd nanoparticles, such a kind of H 2 O 2 biosensor has the potential to be used as an implantable sensor for monitoring H 2 O 2 in vivo . On the other hand, due to the favorable synergistic integration of nanomaterials in the PPy-PtPd nanocomposite, the performance of this simply decorated biosensor should be superior to those conventional metal nanostructures. ,,− ,− …”

“…The changed current was 67.9 μA for 0.4 mM H 2 O 2 , and the sensitivity of 44,45 Based on the signal-to-noise threshold (>3), the limit of detection (LOD) of the present method was set to be 2.5 μM, which is at the medium level of previous reports on H 2 O 2 biosensors. 44,46,47 3.5. Anti-interference Study and Stability Test.…”

Fe-Hemin-Metal Organic Frameworks/Three-Dimensional Graphene Composites with Efficient Peroxidase-Like Bioactivity for Real-Time Electrochemical Detection of Extracellular Hydrogen Peroxide

“…19 Hemin, a wellknown natural metalloporphyrin, is the active center of the family of heme-proteins. 20 Hemin can be used as a promising guest specie for preparing functionalized MOFs. This is because the porphyrin ring can be combined with MOF metal ions, giving them a highly similar structure to the natural enzyme active center.…”