Facile synthesis of chondroitin sulfate-stabilized gold nanoparticles

Li, Wei; Li, Xin; Su, Hui; Zhao, Shiju; Li, Yanyun; Hu, Jiandong

doi:10.1016/j.matchemphys.2010.10.023

Cited by 19 publications

(10 citation statements)

References 37 publications

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“…In the past, various strategies have been used to synthesize gold nanoparticles in organic and aqueous systems. Among these, synthesis of AuNps by citrate reduction is popularly used as it has the advantages of being inexpensive, nontoxic, and is a water‐based system . Optical properties of gold nanoparticles were studied using UV‐Vis Spectra.…”

Section: Resultsmentioning

confidence: 99%

“…It was estimated to be −21.42 mV. The high charge is attributed to the negative sulfated polyanionic groups of CS which wrap around the AuNps and prevent their agglomeration forming stable AuNps‐CS …”

Section: Resultsmentioning

confidence: 99%

“…Among these, synthesis of AuNps by citrate reduction is popularly used as it has the advantages of being inexpensive, nontoxic, and is a water-based system. 30,31 Optical properties of gold nanoparticles were studied using UV-Vis Spectra. The optical spectra of AuNps is shown in Figure 2A.…”

Section: Preparation Of Aunps and Characterizationmentioning

confidence: 99%

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Role of gold nanoparticles as drug delivery vehicles for chondroitin sulfate in the treatment of osteoarthritis

Dwivedi

Nayak

Kowshik

2015

Biotechnol Progress

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Osteoarthritis is a disease which is characterized by joint pain, swelling and stiffness. Articular cartilage has limited self-repair capacity due to its avascular and aneural nature. In this work, we show the use of gold nanoparticles (AuNps) for enhancing the delivery of chondroitin sulfate (CS), a drug used in the treatment of osteoarthritis (OA). AuNps were synthesized and were characterized by transmission electron microscopy (TEM), ultraviolet-visible (UV-Vis) spectroscopy, Fourier transform infrared spectroscopy (FTIR), and X-Ray diffraction analysis. AuNps were combined with CS (AuNps-CS) and their effect on primary goat chondrocytes was studied using MTT assay, Hoechst staining, production of glycosaminoglycan and collagen. Cell viability studies by MTT revealed that AuNps-CS stimulate cell proliferation. A two-fold increase in GAG and collagen production was observed in presence of AuNps-CS combination as compared to native CS, indicating that this combination stimulates chondrocyte proliferation and enhances extracellular matrix production (ECM). Hence, this study exhibits the potential of AuNps as a carrier of CS for treatment of osteoarthritis.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Role of gold nanoparticles as drug delivery vehicles for chondroitin sulfate in the treatment of osteoarthritis

Dwivedi

Nayak

Kowshik

2015

Biotechnol Progress

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“…[117] Poly(lactide-co-glycolide) microspheres PLGA microspheres stabilized by CS formed ionic complexes with positively charged proteins and used for treatment of OA.…”

Section: Subchondral Bonementioning

confidence: 99%

Chondroitin sulphate: a focus on osteoarthritis

et al. 2016

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Chondroitin sulfate (CS) being a natural glycosaminoglycan is found in the cartilage and extracellular matrix. It shows clinical benefits in symptomatic osteoarthritis (OA) of the finger, knee, hip joints, low back, facial joints and other diseases due to its anti-inflammatory activity. It also helps in OA by providing resistance to compression, maintaining the structural integrity, homeostasis, slows breakdown and reduces pain in sore muscles. It is most often used in combination with glucosamine to treat OA. CS is a key role player in the regulation of cell development, cell adhesion, proliferation, and differentiation. Its commercial applications have been continuously explored in the engineering of biological tissues and its combination with other biopolymers to formulate scaffolds which promote and accelerate the regeneration of damaged structure. It is approved in the USA as a dietary supplement for OA, while it is used as a symptomatic slow-acting drug (SYSADOA) in Europe and some other countries. Any significant side effects or overdoses of CS have not been reported in clinical trials suggesting its long-term safety. This review highlights the potential of CS, either alone or in combination with other drugs, to attract the scientists engaged in OA treatment and management across the world.

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“…In order to improve the detectability of these molecules in tissue samples, we have engineered HA-and CS-coated gold nanoparticles (AuNPs) 15 , which have unique surface plasma resonance and optical properties 16 . In this study, we investigated the location of HA and CS after ex vivo incubation of rabbit bladder tissue with HA-AuNPs and CS-AuNPs to determine whether the benefits of HA and CS replacement therapy might be due to coating of the urothelium with GAGs.…”

Section: Introductionmentioning

confidence: 99%

Gold nanoparticles approach to detect chondroitin sulphate and hyaluronic acid urothelial coating

Guelfi

Stefanetti

Zampini

et al. 2017

Sci Rep

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This study investigated the location of hyaluronic acid (HA)- and chondroitin sulphate (CS)-coated gold nanoparticles in rabbit bladder and evaluated gene expression of CD44, RHAMM and ICAM-1 receptors involved in HA and CS transport into the cell. Gold nanoparticles were synthesised by reduction of gold salts with HA or CS to form HA-AuNPs and CS-AuNPs. Bladder samples were incubated with CS-AuNPs and HA-AuNPs or without glycosaminoglycans. Transmission electron microscopy, optic microscopy and scanning electron microscopy were used to determine the location of the synthesised AuNPs. Real-time PCR was used to analyse expression of urothelial cell receptors CD44, RHAMM, ICAM-1, after ex vivo administration of CS-AuNPs and HA-AuNPs. We showed that HA-AuNPs and CS-AuNPs were located in the cytoplasm and tight junctions of urothelial umbrella cells; this appearance was absent in untreated bladders. There were no significant differences in gene expression levels for CD44, RHAMM and ICAM-1 receptors in treated versus control bladder tissues. In conclusion, we clearly showed the presence of exogenous GAGs in the bladder surface and the tight junctions between umbrella cells, which is important in the regeneration pathway of the urothelium. The GAGs-AuNPs offer a promising approach to understanding the biophysical properties and imaging of urothelial tissue.

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Facile synthesis of chondroitin sulfate-stabilized gold nanoparticles

Cited by 19 publications

References 37 publications

Role of gold nanoparticles as drug delivery vehicles for chondroitin sulfate in the treatment of osteoarthritis

Role of gold nanoparticles as drug delivery vehicles for chondroitin sulfate in the treatment of osteoarthritis

Chondroitin sulphate: a focus on osteoarthritis

Gold nanoparticles approach to detect chondroitin sulphate and hyaluronic acid urothelial coating

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