Facile synthesis of high-quality CuInZnxS2+x core/shell nanocrystals and their application for detection of C-reactive protein

Shen, Huaibin; Yuan, Hang; Wu, Feng; Bai, Xianwei; Zhou, Changhua; Wang, Hongzhe; Lu, Tikang; Qin, Zhifeng; Ma, Lan; Li, Lin Song

doi:10.1039/c2jm33763k

Cited by 44 publications

(39 citation statements)

References 43 publications

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“…Therefore, QDs have been successfully utilized for rapid diagnosis. Specifically, QD immune-labeling, which is highly sensitive, quantitative, and reproducible technology that features a wide linear detecting range, has been developed to detect pathogens, characteristic proteins and even nucleic acids Liu et al 2013;Sapountzi et al 2015;Shen et al 2011b;Shen et al 2012;Zhou et al 2011a).…”

Section: Introductionmentioning

confidence: 99%

Multiplexed detection of influenza A virus subtype H5 and H9 via quantum dot-based immunoassay

Yuan

Zhou

et al. 2016

Biosensors and Bioelectronics

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A quantum dot-based lateral flow immunoassay system (QD-LFIAS) was developed to simultaneously detect both influenza A virus subtypes H5 and H9. Water-soluble carboxyl-functionalized quantum dots (QDs) were used as fluorescent tags. The QDs were conjugated to specific influenza A virus subtype H5 and H9 antibodies via an amide bond. When influenza A virus subtype H5 or H9 was added to the QD-LFIAS, the QD-labeled antibodies specifically bound to the H5 or H9 subtype viruses and were then captured by the coating antibodies at test line 1 or 2 to form a sandwich complex. This complex produced a bright fluorescent band in response to 365 nm ultraviolet excitation. The intensity of fluorescence can be detected using an inexpensive, low-maintenance instrument, and the virus concentration directly correlates with the fluorescence intensity. The detection limit of the QD-LFIAS for influenza A virus subtype H5 was 0.016 HAU, and the detection limit of the QD-LFIAS for influenza A virus subtype H9 was 0.25 HAU. The specificity and reproducibility were good. The simple analysis step and objective results that can be obtained within 15 min indicate that this QD-LFIAS is a highly efficient test that can be used to monitor and prevent both Influenza A virus subtypes H5 and H9.

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Section: Introductionmentioning

confidence: 99%

Multiplexed detection of influenza A virus subtype H5 and H9 via quantum dot-based immunoassay

Yuan

Zhou

et al. 2016

Biosensors and Bioelectronics

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“…The reaction mechanism of hydrophilic carboxylfunctionalized CdSe/ ZnS QDs has been previously described in the literature [24,25]. The dynamic light scattering analysis in Figure 2 shows that the average hydrodynamic size of carboxylfunctionalized CdSe/ Zn SQDs was 42.86 nm with Stdev 18.72 nm, and this size increased to 109.5 nm with Stdev 52.16 nm after dihydrazide modified and conjugation with antibodies, which indicated that antibodies were successful conjugated.…”

Section: Conjugation Of Antibodies To Qds Via Oxidized Fccarbohydratementioning

confidence: 75%

Ultra Sensitive Detection of Influenza A Virus Based on Cdse/Zns Quantum Dots Immunoassay

Wu¹,

Mao²,

Liu³

et al. 2016

SOJB

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Open Access Research articlesevere outbreaks disease, and they can cause serious infections among people.To date, numerous analytical methods have being used to detect influenza A virus. According to the type of detection target, these methods could be categorized into, for example, virus isolation and identification, nucleic acid-based detection, antigen detection, and antibody detection [3][4][5][6][7][8]. Most of these methods need demanding conditions and professional operations, and some of the detection processes is time-consuming. For these reasons, some new biosensors based on nano materials such as gold nanoparticles, magnetic nano beads and quantum dots (QDs) have been coming to the forefront [9][10][11][12].Developing a sensitive, specific and fast biosensor for diagnosing the influenza virus at the early stages of infection is a challenge. Lateral flow immune assay method has been widely used in detection of infectious diseases with the advantages of rapid, easy to operate, and low cost [13]. However, traditional method such as colloidal gold lateral flow tests has limitations when high sensitivity is needed [14].QDs have been developed to replace colloidal gold owing to their excellent optical properties such as high fluorescence efficiency, wide range of excitation wavelength, narrow and symmetric emission spectra, and QDs based lateral flow tests have been proved higher sensitivity and reproducibility for detection of pathogen, characteristic protein and even nucleic acid [15][16][17][18].In this paper, we present an ultrasensitive lateral flow immunoassay (LFIA) for detecting influenza A virus. This immune sensor is based on site-specific covalent binding with the Fc end of influenza A virus antibodies to CdSe/ ZnS QDs. The carboxylfunctionalized CdSe/ ZnS QDs conjugated with antibodies via an amide bond often result in random links of the antibody structure in the QD conjugates and block certain antigenbinding sites. In order to obtain site-specific linking, we modified carboxyl-functionalized QDs with adipicdihydrazide (ADH) and oxidized the carbohydrate groups on the antibody's Fc region AbstractAn ultrasensitive lateral flow immunoassay system (LFIAS) was established for the detection of influenza A virus. In this LFIAS, hydrophilic dihydrazide-modified CdSe/ZnSquantom dots (QDs) were conjugated with specific antibodies and used as fluorescent labels, and a pair of matched anti-nucleoprotein of influenza A virus antibodies were used to form a sandwich immunoassay. The QDs were in conjugation with the fragment crystallizable region (Fc region) of specific influenza A virus antibodies through aldehydehydrazide covalent chemistry, conferring high sensitivity. The antibodies used for detection are specific for the most conserved and popular nucleoprotein of influenza A virus and ensure the accuracy and specificity. The QDs-LFIAS can analyze the nasal-pharyngeal swab samples through simple steps and get results within 15 min. Detection of nasal-pharyngeal swab samples makes it more rapid and conve...

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“…4(b), with In 3d 5/2 and In 3d 1/2 at 445 eV and 452.6 eV respectively. The In 3d peaks show a spin-orbit splitting of 7.6 eV which, from previous reports, 56,57 is indicative of In(III). The Zn 2p spectrum is shown in Fig.…”

Section: Spectroscopy Of Inp/zns Ncsmentioning

confidence: 88%

SWCNT photocathodes sensitised with InP/ZnS core–shell nanocrystals

et al. 2016

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Facile synthesis of high-quality CuInZnxS2+x core/shell nanocrystals and their application for detection of C-reactive protein

Cited by 44 publications

References 43 publications

Multiplexed detection of influenza A virus subtype H5 and H9 via quantum dot-based immunoassay

Multiplexed detection of influenza A virus subtype H5 and H9 via quantum dot-based immunoassay

Ultra Sensitive Detection of Influenza A Virus Based on Cdse/Zns Quantum Dots Immunoassay

SWCNT photocathodes sensitised with InP/ZnS core–shell nanocrystals

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