Facile synthesis of Ti4+-immobilized Fe3O4@polydopamine core–shell microspheres for highly selective enrichment of phosphopeptides

Abstract

In this work, for the first time, Ti(4+)-Fe3O4@polydopamine microspheres were designed and synthesized for efficient and selective enrichment of phosphopeptides in biological samples.

Preparation of Ti4+-immobilized modified silica capillary trapping column for on-line selective enrichment of phosphopeptides

“…Immobilized metal affinity chromatography (IMAC) is a mostused method to enrich low-abundance phosphopeptides from complex mixtures of peptides prior to MS analysis. IMAC relies on the high affinity of the phosphate groups with metal ions, such as Ti 4 þ [11][12][13][14][15], Fe 3 þ [16,17], Zr 4 þ [19,20], Zn 2 þ [21] or Ga 3 þ [22]. These materials are believed to have different affinities for phosphopeptides [11,14].…”

“…The metal ions are immobilized on different matrix (polymer beads [23], porous materials [20], nanocomposites [21], graphene [24] or magnetic core-shell architectures [15,25]), using various bridging molecules, such as acidic chelating ligands of phosphate groups, iminodiacetic acid (IDA) [17] or nitrilotriacetic acid (NTA) [16]. Recently, hydrophilic polydopamine (PDA) has successfully been developed [26][27][28] as a novel chelating ligand for indirectly immobilize metal ions on various substrates modified for enrichment of phosphopeptides [15,29,30].…”

Development of magnetic graphene @hydrophilic polydopamine for the enrichment and analysis of phthalates in environmental water samples

“…It could spontaneous polymerize in weak alkaline solution at room temperature. Besides, the generated polydopamine was successfully coated on metal oxides [20,21], polymers [22], carbon nanotubes [23]. And polydopamine has good biocompatibility, great environmental stability and excellent dispersibility in water [24].…”

Preparation of magnetic graphene @polydopamine @Zr-MOF material for the extraction and analysis of bisphenols in water samples

“…Dopamine, which was known as the roles in neuro signal transmission [25], has been paid great attention because of the variety chemical properties. At room temperature, it could be self-polymerized in weak alkaline condition, and be coated on the organic or inorganic surface such as metal oxides, polymers and graphene [26][27][28][29]. Since polydopamine has showed good dispersibility in aqueous matrix and excellent environmental stability [30], we considered combining MOFs with polydopamine to improve the hydrophilicity.…”