Facilitated column selection in pharmaceutical analyses using a simple column classification system

Visky, D.; Haghedooren, Erik; Dehouck, Pieter; Kovács, Zsuzsanna; Kóczián, Kristóf; Noszál, Béla; Hoogmartens, Jos; Adams, Erwin

doi:10.1016/j.chroma.2005.09.069

Cited by 47 publications

(42 citation statements)

References 39 publications

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“…Outlying parameters were determined using the Grubbs' test (a ¼ 0.05) [70]. The conditions of the separations and the list of stationary phases used in each method are described in detail in References [69,[71][72][73][74][75][76].…”

Section: Comparison Of Columns and Application On Real Separationsmentioning

confidence: 99%

Three methods to characterize reversed phase liquid chromatographic columns applied to pharmaceutical separations

Németh

Haghedooren

Noszál

et al. 2008

Journal of Chemometrics

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Selecting a suitable reversed phase liquid chromatographic (RP-LC) column for a particular separation is complicated. More than 600 brands of C 18 columns are available on the market. Therefore, determining the characteristics influencing the selectivity of the different stationary phases is necessary. In this paper, three different methods to characterize RP-LC columns are compared. Hoogmartens et al. developed a column characterization system based on test methods from literature, which were reduced with principal component analysis (PCA), and led to four final chromatographic parameters. The second method studied is this of Euerby et al. who developed a column classification system using six parameters, based on the method of Tanaka and also applying PCA. The third method, developed by Snyder et al. is different from the other two. It uses five parameters, calculated by multiple linear regression from the retention data of 18 solutes. The suitability of the column classification systems was examined on pharmaceutical separations. It seems that all three methods can facilitate the selection of a suitable column for an analysis.

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Section: Comparison Of Columns and Application On Real Separationsmentioning

confidence: 99%

Three methods to characterize reversed phase liquid chromatographic columns applied to pharmaceutical separations

Németh

Haghedooren

Noszál

et al. 2008

Journal of Chemometrics

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“…Chloramphenicol and its glucuronide in some food products (chicken meat, seafood, and honey) have also been determined in 2004 by Bogusz et al by reverse-phase liquid chromatography-electrospray negative ionization mass spectrometry using acetonitrile and ammonium formate 10 mM, pH 3.0 (40 : 60, v/v) as mobile phase at flow rate of 0.3 mL/min [8]. In 2006, Visky et al have described an HPLC method for the separation of seven different drug substances from their respective impurities, among these drug substances is chloramphenicol and its impurities [9].…”

Section: Introductionmentioning

confidence: 99%

Analysis of Chloramphenicol and Its Related Compound 2-Amino-1-(4-nitrophenyl)propane-1,3-diol by Reversed-Phase High-Performance Liquid Chromatography with UV Detection

Al‐Rimawi

Kharoaf²

2011

Chromatography Research International

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A simple and stability-indicating liquid chromatographic method is developed for the analysis of chloramphenicol and its related compound 2-amino-1-(4-nitrophenyl)propane-1,3-diol in two pharmaceutical forms. Liquid chromatography with a UV detector at a wavelength of 278 nm using a reversed phase C18 stationary phase has been employed in this study. Isocratic elution is employed using a mixture of sodium pentanesulfonate solution (0.012 M), acetonitrile, and glacial acetic acid (85 : 15 : 1, v/v). This new method is validated in accordance with USP requirements for new methods for assay determination, which include accuracy, precision, specificity, linearity and range. This method shows enough selectivity, sensitivity, accuracy, precision, and linearity range to satisfy Federal Drug Administration/International Conference on Harmonization regulatory requirements. The current method demonstrates good linearity over the range of 0.04-0.16 mg/mL of chloramphenicol. The accuracy of the method is 100.0% with a relative standard deviation of 0.1%. The precision of this method reflected by relative standard deviation of replicates is 0.1%. The method is sensitive with a detection limit of 0.005% for chloramphenicol. The related substance of chloramphenicol (2-amino-1-(4-nitrophenyl)propane-1,3-diol) can be selectively determined with a good resolution in two pharmaceutical forms: eye ointment and eye drops.

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“…The residual combination of parameters was used in subsequent publications for testing the similarity and dissimilarity of columns for use in a particular assay (e. g., see ref. [77,78]). A summary of the method, which is carried out in three different mobile phases, has been reported in 2006 [79].…”

Section: General Characterization Methods For Stationary Phasesmentioning

confidence: 97%

Stationary phase characterization and method development

Neue

2007

J of Separation Science

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The properties of stationary phases and their characterization methods are reviewed. New and significant developments have occurred in the last few years, and new methods for stationary phase characterization have become available. The characterization methods are discussed, and the differences between the different methods are pointed out. In addition, method development approaches are reviewed, with special emphasis on recent developments that employ multiple parameters in parallel. Also, the renewed interest of temperature as a tool in method development is surveyed.

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Facilitated column selection in pharmaceutical analyses using a simple column classification system

Cited by 47 publications

References 39 publications

Three methods to characterize reversed phase liquid chromatographic columns applied to pharmaceutical separations

Three methods to characterize reversed phase liquid chromatographic columns applied to pharmaceutical separations

Analysis of Chloramphenicol and Its Related Compound 2-Amino-1-(4-nitrophenyl)propane-1,3-diol by Reversed-Phase High-Performance Liquid Chromatography with UV Detection

Stationary phase characterization and method development

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