Although the home working model, which started in the general directorates at first and then emerged in the branches in the banking sector, has some advantages, it disrupts the work-life balance of individuals in the long run. Thus, the present study focused on the moderating role of professional self-efficacy in the relationship between the perceived organizational support and work-life balance of women employed in private banks operating in Turkey and working from home. To this end, data were collected from 403 female private bank employees by the survey method and tested with the help of the Smart PLS 3 analysis program. According to the study findings, women's professional self-efficacy beliefs play a moderating role in the relationship between perceived organizational support and work-life balance. This relationship has been ignored in the literature. Therefore, this study enriches the content related to organizational support theory and social cognitive theory by investigating the relationship between perceived organizational support, professional self-efficacy, and work-life balance in women working in the banking sector from home and thus contributes to theory and practice.