Factors influencing plant regeneration from seedling explants of Hairy nightshade (Solanum sarrachoides)

Ju, Ho-Jong; Eck, Joyce Van; Gray, Stewart M.

doi:10.1007/s11240-011-0020-x

Cited by 4 publications

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References 36 publications

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“…It ranged from the highly responsive proximal part where hypocotyl sections adjacent to the cotyledon gave the highest number of shoots (Fári and Czako 1981;Shang et al 2006;Sharma et al 2011;Wang et al 2011) to the hypocotyl culture where the bottom parts of the hypocotyl (closer to the root) produced more shoots (Nagori and Purihit 2004;Chen et al 2008). The different responses of hypocotyl segments may be due to variation in the endogenous concentration of PGRs in the mother plants of different plant species (George 1993;Ju et al 2012). Also, germination conditions of seeds such as temperature and light can significantly influence the subsequent morphogenetic response in culture in vitro.…”

Section: In Vitro Plant Propagation and Acclimatizationmentioning

confidence: 99%

Advancement in protocol for in vitro seed germination, plant regeneration and cryopreservation of Viola cornuta

et al. 2019

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The aim of this study was to develop a fast, reliable and true-to-type protocol for in vitro plant regeneration and long-term storage of horned pansy (Viola cornuta L). Seed germination over 60% was recorded after 12 weeks of growth at 10 °C or 4 °C. Calli formation and shoot induction were obtained in petiole and hypocotyl culture on half-strength MS mineral salts with full concentration of Na-FeEDTA and vitamins (½MS medium) with 2,4-dichlorophenoxyacetic acid (2,4-D, 0.1 mg/L) and 6-benzylaminopurine (BAP, 2.0 mg/L) and leaf culture on ½MS medium with thidiazuron (TDZ,1.0 mg/L). The highest frequency of adventitious shoot induction (50%) with six shoots/explant was achieved in hypocotyl culture from top hypocotyl segments, close to epicotyl which was grown 8 weeks at 16 h light/8 h dark photoperiod. Subsequent shoot multiplication was achieved on ½MS medium with α-naphthaleneacetic acid (NAA, 0.1 or 0.5 mg/L) and BAP (1.0 mg/L). Rooting of shoots was obtained on ½MS medium with low concentration (0.1 mg/L) of auxins: indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) or NAA, or without growth regulators. In vitro-derived plantlets were acclimatized under greenhouse conditions. All plants developed normally, bloomed and set seeds. Shoot tips were cryopreserved succssefully using modified plant vitrification 3 (PVS3-based vitrification procedure). Cold acclimation for 2 weeks significantly improved shoot regrowth (64%) after thawing in comparison to non-acclimated shoots (39%). Clonal fidelity of regenerated plantlets at ploidy level was confirmed by chromosome counting. The presented protocol can be useful for mass propagation, genetic transformation studies and long-term storage of valuable Viola spp.

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