Failure of Soybean Trypsin Inhibitor to Exert Deleterious Effects in Calves

Kakade, M. L.; Thompson, Robert D.; Engelstad, W.E.; Behrens, Gina; Yoder, Ralph Daniel; Crane, F.M.

doi:10.3168/jds.s0022-0302(76)84388-3

Cited by 40 publications

(12 citation statements)

References 13 publications

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“…In our experi ments 1 and 2, various treatments involving steam-heating, water or alcohol extraction of oligosides, protein extraction and hydrolysis, resulting in different antigenic activities, were applied to soya bean. Then, the pancreas did not seem to be sensitive to protease inhibitors as previously reported by Kakade et al [ 10] in the calf and Naim et al [13] in the rat. There fore, the effects of soya bean products on the relative development of the pancreas are vari able and difficult to foresee.…”

Section: Liveweight Gain and Pancreatic Developmentsupporting

confidence: 67%

“…According to these authors, this could be partially due to the presence of resid ual protease inhibitors which were also pres ent in the raw pea diet as well as in most soya bean flours and concentrates in our study. However, Kakade et al [10] recorded no effect of purified soya bean trypsin inhibitors added (during 12 days) to heated soya bean meal on enzyme activities in 15-day-old calf pancreas. Trypsin inhibitor sensitivity in calves could be different with age since Le Huerou et al [40] showed that total protein synthesis and enzyme activity in the pancreas still varied during the first month of life.…”

Section: Liveweight Gain and Pancreatic Developmentmentioning

confidence: 99%

“…Gorrill et al [9], Temouth et al [19], Guilloteau et al [11] and Khorasani et al [20] showed a decrease in the pancreatic content and/or secretion in calves given diets contain ing soya bean products while Kakade et al [10] did not observe any change in either pro tein concentration or enzyme activities in the pancreatic tissue. These contradictory results could be explained by the use of different experimental methods, but also by the nature of soya bean products and the treatments applied to them.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Response of the Calf Pancreas to Differently Processed Soya Bean and Pea Diets

Dréan¹,

Huërou-Luron²,

Philouze-Romé³

et al. 1995

Ann Nutr Metab

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The purpose of this study was to determine the effect of replacing skim-milk powder by differently treated soya bean or pea products on growth, pancreas size and pancreatic enzyme activities in calves. Three separate experiments have been performed. In experiments 1 and 2, 28 and 21 male Holstein calves were divided into 4 or 3 groups, respectively, and fed either dairy products or milk substitutes in which protein was mainly provided by soya bean products differing in their protein concentration due to the technological processing applied. In experiment 3, 45 male Holstein calves were divided into 3 groups and were fed either dairy products, or raw or flaked pea flour as a protein source. After an experimental period of 99 ± 4 days in experiments 1 and 2, and of 88 days in experiment 3, animal growth rate was significantly lower with raw pea flour (16%) and with the soya bean diet, which was highly concentrated in carbohydrates and allergenic proteins (13-27%). Pancreas weight decreased significantly (16-18%) with pea diets and tended to be lower (NS) with the water extracted, concentrated and heated flour (soya bean). Amylase-specific activity increased significantly (43%) with pea diets but showed opposite tendencies with the most refined soya bean products. Proteolytic enzyme activities were slightly influenced by dietary protein source, but this was not as obvious as in the literature reviewed. Specific messenger RNAs corresponding to amylase, trypsin and chymotrypsin seemed to increase (NS) with the soya bean diets, particularly with the less elaborated one. However, further investigations are required before any conclusions may be drawn concerning regulation levels of pancreatic adaptation to dietary protein. According to this study and the literature, results concerning pancreatic response to diets were different suggesting that the origin of soya bean, pea seeds and technological treatments applied to them were of great importance. Also, the level of incorporation into milk substitute and the presence of more or less antinutritional factors could influence pancreatic enzyme variations by complex mechanisms.

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Section: Liveweight Gain and Pancreatic Developmentsupporting

confidence: 67%

Section: Liveweight Gain and Pancreatic Developmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Response of the Calf Pancreas to Differently Processed Soya Bean and Pea Diets

Dréan¹,

Huërou-Luron²,

Philouze-Romé³

et al. 1995

Ann Nutr Metab

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“…The trypsin inhibitory fraction of raw green gram was isolated by the method of Kakade et al [11] with slight modification, as illustrated in our previous publication [27].…”

Section: Biological Experiments With Rats Preparation Of Green Gram Tmentioning

confidence: 99%

Effect of dietary green gram trypsin inhibitor and raw green gram meal (Phaseolus aureus roxb) on the pancreatic activity in rats

1982

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To evaluate the effect of green gram trypsin inhibitor (GGTI) and raw green gram meal (RGG) the experimental animals were fed with the following diets: (i) AGG meal-control (autoclaved green gram meal), (ii) RGG meal and (iii) AGG meal + 1% GGTI. Pancreatic enlargement was observed in rats fed RGG diet and AGG + GGTI diet. Elevated levels of protease and amylase were observed in pancreas. Intestinal contents showed no significant variation in the protease activity, but a significant variation in the amylase activity was evident. Total RNA, DNA and protein content were increased in the pancreas of group II and group III set of animals, suggesting an active proliferation of acinar cells. RNA and DNA content per unit weight of pancreas and the RNA/DNA ratio remained almost constant indicating the hyperplasia. Histological studies revealed hyperplasia of the acinar cells. The results obtained have been discussed.

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“…Esta perda de peso pode ser relacionada à habilidade inferior do sistema digestivo em digerir e absorver os nutrientes, à presença do fator inibidor da tripsina, ou à redução das secreções proteolíticas do trato digestivo, conforme descrito (Kakade et al 5 O exame histopatológico dos segmentos do intestino delgado dos bezerros alimentados com EHS revelou diminuição significativa (p < 0,05) no tamanho das vilosidades intestinais, em relação aos bezerros alimentados com SL (6,17 cm x 2,32 cm).…”

Section: T Ab Elaunclassified

Alimentação de bezerros com extrato hidrossolúvel de soja. II. Produção de anticorpos e distúrbios intestinais

Esteves

Penteado

Ortolani

et al. 1996

Braz. J. Vet. Res. Anim. Sci.

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Alimentação de bezerros com extrato hidrossolúvel de soja. II. Produção de anticorpos e distúrbios intestinais RESUMOO objetivo do trabalho foi avaliar a substituição total do sucedâneo lácteo à base de proteína de leite (SL) por extrato hidrossolúvel de soja (EHS) na alim entação de bezerros Holandeses machos, com idade média inicial de cinco dias. Os bezerros alimentados com EHS a partir do 5° dia de vida apresentaram, com o desenvolver do experimento, perda de peso pronunciada, diarréia, letargia, caquexia e, nos estágios terminais, desidratação e hipoproteinemia. Apresentaram, ainda, atrofia das vilosidades intestinais e produção de anticorpos no soro contra a proteína do EHS, indicação de que os bezerros estavam padecendo de uma reação alérgica à proteína de soja, enquanto os bezerros do grupo-controle apresentaram -se com desenvolvimento corporal normal. Os bezerros alimentados com EHS apresentaram diminuição visível das gorduras presentes no tecido subcutâneo, omento, epíplon e da gordura perirenal. No abomaso, a principal alteração verificada foi a falta de form ação do coágulo do EHS ingerido.

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Failure of Soybean Trypsin Inhibitor to Exert Deleterious Effects in Calves

Cited by 40 publications

References 13 publications

Response of the Calf Pancreas to Differently Processed Soya Bean and Pea Diets

Response of the Calf Pancreas to Differently Processed Soya Bean and Pea Diets

Effect of dietary green gram trypsin inhibitor and raw green gram meal (Phaseolus aureus roxb) on the pancreatic activity in rats

Alimentação de bezerros com extrato hidrossolúvel de soja. II. Produção de anticorpos e distúrbios intestinais

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