Prevention of HIV transmission from mother to child or Prevention of Mother to Child Transmission (PMTCT) is part of efforts to control HIV-AIDS and Sexually Transmitted Infections (STIs). In Indonesia, there were 1,805,993 pregnant women tested for HIV and there were 5,074 (0.28%) pregnant women who were HIV positive. The purpose of this study was to analyze the determinants of PMTCT examination with the Health Believe Model approach in Makassar City in 2019. This study was an observational analytic study with a cross-sectional design. The sample in this study was 260 pregnant women in the working area of the Sudiang Raya Health Center and Antang Perumnas Makassar City. Data were analyzed using chi square and multiple logistic regression. The results showed that the cues acted influentially on PMTCT examination with p-value = 0.014 (p <0.05) with OR = 6.013, and there were some insignificant variables such as threat perception variable with p-value = 1,000 (p> 0, 05), the perception of the benefits of p-value = 0.143 (p> 0.05) and the perception of resistance p-value = 0.340 (p> 0.05). It was concluded that the cues acted influentially on the PMTCT examination and to the perception of threats, perceived benefits, and perceived barrier did not affect the PMTCT examination in Makassar City. It is expected that health workers explain in advance about the actions that will be taken (PMTCT examination), and provide information about the importance of efforts to prevent HIV transmission from mother to baby.