Faraday cage-type aptasensor for dual-mode detection of Vibrio parahaemolyticus

Wei, Wenting; Lin, Han; Shao, Huili; Hao, Tingting; Wang, Sui; Hu, Yufang; Guo, Zhiyong; Su, Xiurong

doi:10.1007/s00604-020-04506-1

Cited by 27 publications

(2 citation statements)

References 38 publications

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“…Bacteria may cause great harm to health while existing in the human circulatory system, indicating the importance of sensitive detection. Utilizing steric hindrance on electron transfer, Vibrio parahaemolyticus [121] and Escherichia coli [122] can be successfully analyzed with ECL sensors based on Ru-MOF and NH 2 -MIL-53(Al) signal reporters, respectively. In addition, an exosome as a subcellular structure is also accurately detected using well-designed ECL sensors with a different signal transduction.…”

Section: Cellular Analysismentioning

confidence: 99%

Framework-Enhanced Electrochemiluminescence in Biosensing

Hou

et al. 2023

Chemosensors

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Electrochemiluminescence (ECL) has attracted increasing attention owing to its intrinsic advantages of high sensitivity, good stability, and low background. Considering the fact that framework nanocrystals such as metal–organic frameworks and covalent organic frameworks have accurate molecular structures, a series of framework-based ECL platforms are developed for decoding emission fundamentals. The integration of fluorescent ligands into frameworks significantly improves the ECL properties due to the arrangement of molecules and intramolecular electron transfer. Moreover, the various framework topologies can be easily functionalized with the recognition elements to trace the targets for signal readout. These ECL enhancement strategies lead to a series of sensitive analytical methods for protein biomarkers, DNA, small biomolecules, and cells. In this review, we summarize recent advances in various functions of frameworks during the ECL process, and constructions of framework-based ECL platforms for biosensing. The framework-based ECL nanoemitters and enhancement mechanisms show both theoretical innovation and potential applications in designing ECL biosensing systems. Perspectives are also discussed, which may give a guideline for researchers in the fields of ECL biosensing and reticular materials.

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Section: Cellular Analysismentioning

confidence: 99%

Framework-Enhanced Electrochemiluminescence in Biosensing

Hou

et al. 2023

Chemosensors

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“…Guo’s group prepared a Faraday cage-type ECL sensor based on MXene for detecting the transgenic component Cry1Ab protein . They also developed ruthenium-based metal organic frameworks as the Faraday cage for the detection of vibrio parahaemolyticus . Faraday cage-type ECL sensors with extraordinary accuracy and sensitivity had broad development prospects in the field of ECL sensing.…”

mentioning

confidence: 99%

Accurate Capture and Identification of Exosomes: Nanoarchitecture of the MXene Heterostructure/Engineered Lipid Layer

Nie

Wang

et al. 2023

ACS Sens.

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Recently, exosome detection has become an important breakthrough in clinical diagnosis. However, the effective capture and accurate identification of cancer exosomes in a complex biomatrix are still a tough task. Especially, the large size and non-conductivity of exosomes are not conducive to highly sensitive electrochemical or electrochemiluminescence (ECL) detection. Therefore, we have developed a Ti3C2T x –Bi2S3–x heterostructure/engineered lipid layer-based nanoarchitecture to overcome the limitations. The engineered lipid layer not only specifically captured and efficiently fused CD63 positive exosomes but also showed excellent antifouling property in the biological matrix. Moreover, the MUC1 aptamer-modified Ti3C2T x –Bi2S3–x heterostructure further identified and covered the gastric cancer exosomes that have been trapped in the engineered lipid layer. In the self-luminous Faraday cage-type sensing system, the Ti3C2T x –Bi2S3–x heterostructure with sulfur vacancies extended the outer Helmholtz plane and amplified the ECL signal. Therefore, this sensor can be used to detect tumor exosomes in ascites of cancer patients without additional purification. It provides a new pathway to detect exosomes and other large-sized vesicles with high sensitivity.

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