FASCIATA Genes for Chromatin Assembly Factor-1 in Arabidopsis Maintain the Cellular Organization of Apical Meristems

Kaya, Hidetaka; Shibahara, Kei-ichi; Taoka, Ken‐ichiro; Iwabuchi, Masaki; Stillman, Bruce; Araki, Takashi

doi:10.1016/s0092-8674(01)00197-0

Cited by 440 publications

(502 citation statements)

References 44 publications

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“…GUS expression analysis was performed basically as previously described (Kaya et al 2001). Plants were incubated in reaction buffer containing potassium ferrocyanide and potassium ferricyanide to minimize diffusion artifacts and to enable observation of clear clonal sectors.…”

Section: Histochemical Detection Of Gus Expressionmentioning

confidence: 99%

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Chromatin assembly factor 1 ensures the stable maintenance of silent chromatin states in Arabidopsis

Ono¹,

Kaya²,

Takeda³

et al. 2006

Genes to Cells

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Newly synthesized DNA is rapidly assembled into mature nucleosomes by the deposition of preexisting and nascent histones, and some parts of this process are facilitated by chromatin assembly factor 1 (CAF-1). Loss-of-function mutants of CAF-1 in Arabidopsis , fasciata ( fas ), show a variety of morphological abnormalities and unique defects in gene expression in the meristems. In order to clarify the implications of CAF-1 in the maintenance of chromatin states in higher eukaryotes, we investigated transcriptional gene silencing (TGS) of various genes in fas mutants. Here, we show that TGS of endogenous CACTA transposons was released in a stochastic manner in fas . Other endogenous silent genes, a transposon AtMu1 and a hypothetical gene T5L23.26 at a heterochromatin knob, were also transcriptionally activated, and the activation of the three different silent loci at different chromosomal sites occurred non-concomitantly with each other. Furthermore, TGS of the silent β β β β -glucuronidase ( GUS ) transgene was also de-repressed randomly in fas . We conclude that CAF-1 ensures the stable inheritance of epigenetic states through growth and development in Arabidopsis .

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Section: Histochemical Detection Of Gus Expressionmentioning

confidence: 99%

“…These mutants exhibit essentially the same morphological abnormalities including fasciation, reduced root growth, and disorganization of meristems (Leyser & Furner 1992;Kaya et al . 2001).…”

Section: Introductionmentioning

confidence: 99%

Chromatin assembly factor 1 ensures the stable maintenance of silent chromatin states in Arabidopsis

Ono¹,

Kaya²,

Takeda³

et al. 2006

Genes to Cells

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“…In A. thaliana, AtMSI1 is associated with the CAF complex 22 and with PcG complexes, such as MEDEA 21 . Additionally, MSI-like proteins associate with retinoblastoma in animal and plant systems, and ZmRbAp1, the maize FVE ortholog, interacts in vitro with maize retinoblastoma protein ZmRBR1 (ref.…”

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confidence: 99%

Regulation of flowering time by FVE, a retinoblastoma-associated protein

et al. 2004

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The initiation of flowering in plants is controlled by environmental and endogenous signals 1,2 . Molecular analysis of this process in Arabidopsis thaliana indicates that environmental control is exerted through the photoperiod and vernalization pathways, whereas endogenous signals regulate the autonomous and gibberellin pathways. The vernalization and autonomous pathways converge on the negative regulation of FLC 3,4 , a gene encoding a MADS-box protein that inhibits flowering 3,4 . We cloned FVE, a component of the autonomous pathway that encodes AtMSI4, a putative retinoblastomaassociated protein. FVE interacted with retinoblastoma protein in immunoprecipitation assays, and FLC chromatin was enriched in acetylated histones in fve mutants. We conclude that FVE participates in a protein complex repressing FLC transcription through a histone deacetylation mechanism. Our data provide genetic evidence of a new developmental function of these conserved proteins and identify a new genetic mechanism in the regulation of flowering. shown in the upper part, capital letters correspond to the wild-type or mutated nucleotides. In the protein schematic, gray boxes represent WD repeats, the narrow black box represents a putative nuclear localization signal and the asterisk indicates a putative retinoblastoma-binding motif.

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“…Temperature sensitive mutants of PCNA have been characterized in Drosophila, all of them strongly suppress PEV (position effect variegation), which reveals a role for insect PCNA in chromatin assembly and modification (Henderson et al, 1994). Unlike Drosophila and mammals, in which CAF-1 is essential for survival, plants harboring deletions of either FAS1 or FAS2 (Arabidopsis CAF-1 p150 and p60) are viable (Kaya et al, 2001). Interestingly, knockout of Arabidopsis FAS1 or FAS2 disrupts the integrity of cellular organization in shoot apical meristem and root apical meristem.…”

Section: The Role Of Replication Coupled Nucleosome Assembly In Chrommentioning

confidence: 99%

“…Interestingly, knockout of Arabidopsis FAS1 or FAS2 disrupts the integrity of cellular organization in shoot apical meristem and root apical meristem. Functional study suggests fas mutants fail to maintain the expression of some meristem specific genes essential for its identity (Kaya et al, 2001), suggesting a critical role for CAF-1 in maintaining gene expression profile in proliferating cells.…”

Section: The Role Of Replication Coupled Nucleosome Assembly In Chrommentioning

confidence: 99%

Nucleosome assembly and epigenetic inheritance

2010

Protein Cell

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In eukaryotic cells, histones are packaged into octameric core particles with DNA wrapping around to form nucleosomes, which are the basic units of chromatin (Kornberg and Thomas, 1974). Multicellular organisms utilise chromatin marks to translate one single genome into hundreds of epigenomes for their corresponding cell types. Inheritance of epigenetic status is critical for the maintenance of gene expression profile during mitotic cell divisions . During S phase, canonical histones are deposited onto DNA in a replication-coupled manner . To understand how dividing cells overcome the dilution of epigenetic marks after chromatin duplication, DNA replication coupled (RC) nucleosome assembly has been of great interest. In this review, we focus on the potential influence of RC nucleosome assembly processes on the maintenance of epigenetic status.

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FASCIATA Genes for Chromatin Assembly Factor-1 in Arabidopsis Maintain the Cellular Organization of Apical Meristems

Cited by 440 publications

References 44 publications

Chromatin assembly factor 1 ensures the stable maintenance of silent chromatin states in Arabidopsis

Chromatin assembly factor 1 ensures the stable maintenance of silent chromatin states in Arabidopsis

Regulation of flowering time by FVE, a retinoblastoma-associated protein

Nucleosome assembly and epigenetic inheritance

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