Fast chlorophyll a fluorescence induction (OJIP) phenotyping of chlorophyll-deficient wheat suggests that an enlarged acceptor pool size of Photosystem I helps compensate for a deregulated photosynthetic electron flow

Ferroni, Lorenzo; Živčák, Marek; Kovár, Marek; Colpo, Andrea; Pancaldi, Simonetta; Allakhverdiev, Suleyman I.; Brestič, Marián

doi:10.1016/j.jphotobiol.2022.112549

Cited by 21 publications

(33 citation statements)

References 90 publications

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“…The continuous events are reflected in the fluorescence multiphase rise process. The double hit method was used for the determination (Ferroni et al, 2022). In short, the leaves were dark acclimated for 30 min, and clamped by the leaf clip of the instrument.…”

Section: Determination Of the In Vivo Chlorophyll Fluorescence Phenot...mentioning

confidence: 99%

CsCuAO1 associated with CsAMADH1 confers drought tolerance by modulating GABA levels in the tea plant

Zhu

Cao

Zhang

et al. 2023

Preprint

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Our previous study has shown that copper-containing amine oxidase ( CuAO) and aminoaldehyde dehydrogenase ( AMADH) could regulate the accumulation of γ-aminobutyric acid (GABA) in tea plants by participating in polyamine degradation pathway. However, the involvement of these genes in drought tolerance has not been underlined. In this study, CsCuAO1 associated with CsAMADH1 confers drought tolerance, which modulates GABA levels in tea plants, was conducted. The results showed that exogenous GABA spraying effectively alleviated the drought induced physical damage. Overexpression Arabidopsis lines of CsCuAO1 and CsAMADH1 exhibited enhanced resistance to drought, which promoted the synthesis of GABA and putrescine, by alerting reactive oxygen species scavenging capacity and stomatal movement. However, suppression of CsCuAO1 or CsAMADH1 in tea plants exhibited increased sensitivity during drought treatment. Moreover, the co-overexpressed plants increased the accumulation of GABA both in the Agrobacterium-mediated Nicotiana benthamiana transient assay and transgenic Arabidopsis plants. In addition, a GABA transporter, CsGAT1, was identified, whose expression is strongly correlated with GABA accumulation levels in different tissues under drought stress. Taken together, CsCuAO1 and CsAMADH1 were involved in response to drought stress through a dynamic balance between GABA and putrescine. Our data will greatly contribute to the characterization of GABA biological functions in response to environmental stresses in plants.

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Section: Determination Of the In Vivo Chlorophyll Fluorescence Phenot...mentioning

confidence: 99%

CsCuAO1 associated with CsAMADH1 confers drought tolerance by modulating GABA levels in the tea plant

Zhu

Cao

Zhang

et al. 2023

Preprint

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“…Chlorophyll-depleted mutants often exhibit peculiar features in their photosynthetic membranes, among which is an altered stoichiometry between photosystem I and II (PSI and PSII); this, in turn, causes an imbalance in the excitation rate between PSI and PSII, an abnormal organization of the thylakoids, changes in the spatial distribution of the two photosystems, and reduced control of photosynthetic electron flow, resulting in delayed/reduced growth and increased sensitivity to environmental factors [ 14 , 15 , 16 , 17 , 18 , 19 ]. The chlorophyll-deficient wheat lines used in this study are derived from wild-type cultivars of Triticum durum L. (durum wheat LD222) and Triticum aestivum L. (bread wheat Novosibirskaya 67, NS67), and have been extensively characterized for the structure, organization, and functionality of their photosynthetic apparatus: ANDW-7A, ANDW-8A and ANDW-7B (7A, 8A, 7B) for durum wheat, and ANBW-4A, ANBW-4B and ANK-32A (4A, 4B, 32A) for bread wheat ( Table A1 ).…”

Section: Introductionmentioning

confidence: 99%

“…A chronic overreduction of the electron transport chain can be extremely harmful for plants and even threaten their survival [ 21 ]. Nevertheless, the chlorophyll-depleted wheat mutants cope with the electron-flow deregulation through the accumulation of PSI end electron acceptors (ferredoxin and ferredoxin-NADP + -oxidoreductase, FNR) and, in addition, the enhancement of the electron-sink alternative to photosynthesis and photorespiration [ 15 , 16 ]. These compensative adjustments inevitably affect the biomass yield of the mutants, which is generally lower than that of their wild-type lines [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

“…Up until now, the relevant information about this collection of mutants for potential field cultivation can be summarized as follows: The phenotype severity tends to decrease during the life cycle of the plants cultivated indoors; i.e., the leaf chlorophyll content recovers to wild-type-like levels (phenotype alleviation) [ 14 ]; The phenotype alleviation is incomplete outdoors with a short (March–June) life cycle [ 14 ]; The phenotype alleviation can be incomplete indoors if plants are cultivated under fluctuating light [ 15 , 16 ]. …”

Section: Introductionmentioning

confidence: 99%

“…The phenotype alleviation can be incomplete indoors if plants are cultivated under fluctuating light [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

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Long-Term Alleviation of the Functional Phenotype in Chlorophyll-Deficient Wheat and Impact on Productivity: A Semi-Field Phenotyping Experiment

Colpo

Demaria

Baldisserotto

et al. 2023

Plants

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Wheat mutants with a reduced chlorophyll synthesis are affected by a defective control of the photosynthetic electron flow, but tend to recover a wild-type phenotype. The sensitivity of some mutants to light fluctuations suggested that cultivation outdoors could significantly impact productivity. Six mutant lines of Triticum durum or Triticum aestivum with their respective wild-type cultivars were cultivated with a regular seasonal cycle (October–May) in a semi-field experiment. Leaf chlorophyll content and fluorescence parameters were analysed at the early (November) and late (May) developmental stages, and checked for correlation with morphometric and grain-production parameters. The alleviation of the phenotype severity concerned primarily the recovery of the photosynthetic-membrane functionality, but not the leaf chlorophyll content. Photosystem II (PSII) was less photoprotected in the mutants, but a moderate PSII photoinhibition could help control the electron flow into the chain. The accumulation of interchain electron carriers was a primary acclimative response towards the naturally fluctuating environment, maximally exploited by the mature durum-wheat mutants. The mutation itself and/or the energy-consuming compensatory mechanisms markedly influenced the plant morphogenesis, leading especially to reduced tillering, which in turn resulted in lower grain production per plant. Consistently with the interrelation between early photosynthetic phenotype and grain-yield per plant, chlorophyll-fluorescence indexes related to the level of photoprotective thermal dissipation (pNPQ), photosystem II antenna size (ABS/RC), and pool of electron carriers (Sm) are proposed as good candidates for the in-field phenotyping of chlorophyll-deficient wheat.

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Research on the Differences in Phenotypic and Photosynthetic Biophysical Parameters of Begonias (Begonia rex) Cultivars Under Various Light Spectral Compositions

Kazemi,

Dehestani-Ardakani,

Hatami

et al. 2023

J Plant Growth Regul

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Fast chlorophyll a fluorescence induction (OJIP) phenotyping of chlorophyll-deficient wheat suggests that an enlarged acceptor pool size of Photosystem I helps compensate for a deregulated photosynthetic electron flow

Cited by 21 publications

References 90 publications

CsCuAO1 associated with CsAMADH1 confers drought tolerance by modulating GABA levels in the tea plant

CsCuAO1 associated with CsAMADH1 confers drought tolerance by modulating GABA levels in the tea plant

Long-Term Alleviation of the Functional Phenotype in Chlorophyll-Deficient Wheat and Impact on Productivity: A Semi-Field Phenotyping Experiment

Research on the Differences in Phenotypic and Photosynthetic Biophysical Parameters of Begonias (Begonia rex) Cultivars Under Various Light Spectral Compositions

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