In this paper, we present a new attempt to lay ballastless tracks on long-span cable-stayed bridges on high-speed railways. The arrangements of ballastless tracks laid on cable-stayed bridges can be divided into two conditions: (i) across the cable suspension-point cross-section or (ii) in discontinuity at the cable suspension-point cross-section. At present, there is a lack of in-depth research on ballastless tracks laid on long-span cable-stayed bridges, especially on the mechanical behaviors and fatigue performances of the ballastless tracks with different arrangements. For this paper, a segmental model of a long-span cable-stayed bridge was designed and built, on which full-scale ballastless tracks with two different arrangements were arranged. A series of fatigue tests and post-fatigue loading tests were carried out based on the two selected full-scale ballastless tracks. Some conclusions were drawn as follows. For the longitudinal end of the ballastless track, which is far from the loading positions, the interlayers of the ballastless tracks tend to warp up relatively, and the compressive pressures at the interlayers are also unloaded. However, there is no void or gap formed at the interlayers of the longitudinal end of the track slab due to the precompression of the rubber isolation layer. For the center of the track slab, which is close to the loading positions, the compressive deformations occur at the interlayers, and the pressures at interlayers are also increased. The maximum compressive deformation is less than 0.5 mm under the standard train axle load (170 kN), and it cannot affect the high-speed trainsâ operation. With the increase of the post-fatigue loading, the load-displacement curves and the load-pressure variation curves of the ballastless tracks show apparent nonlinearity. Moreover, with the increase of the fatigue loading cycles, the compressive stiffness enhancement or degradation of the ballastless tracks are not noticeable. That is to say, the ballastless tracks laid on the long-span cable-stayed bridges with different arrangements have good mechanical behaviors, and their fatigue performances can also be guaranteed after bearing repeated loadings.