Fatty acid metabolism and population dynamics in a wet biowaste digester during re-start after revision

Moertelmaier, Christoph; Li, Chaoran; Winter, Josef; Gallert, Claudia

doi:10.1016/j.biortech.2014.05.085

Cited by 22 publications

(13 citation statements)

References 23 publications

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“…On the contrary, in AD of wastewater sludge, we observed the lowest cell numbers accounting for 1.97 × 10 8 bacterial cells per ml. In line with previous quantitative detection of SPOB in methanogenic environments based on qPCR or FISH (Ariesyady et al, 2007;Narihiro et al, 2012;Li et al, 2014;Moertelmaier et al, 2014;Mathai et al, 2015), comparable abundances of Pelotomaculum, Syntrophobacter and Smithella were identified accounting for 0.7%-7.3%, 0.1%-1.3% and 0%-6.9% of all bacterial cells respectively. Relative abundances of SPOB quantified by qPCR were in good coherence with the sequencing-based 16S rRNA gene survey ( Supplementary Information Table S4).…”

Section: Genome Properties and Mechanisms For Syntrophysupporting

confidence: 87%

Candidatus Syntrophosphaera thermopropionivorans: a novel player in syntrophic propionate oxidation during anaerobic digestion

Dyksma

Gallert

2019

Environ Microbiol Rep

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Summary Propionate is an important intermediate in the anaerobic mineralization of organic matter. In methanogenic environments, its degradation relies on syntrophic associations between syntrophic propionate‐oxidizing bacteria (SPOB) and Archaea. However, only 10 isolated species have been identified as SPOB so far. We report syntrophic propionate oxidation in thermophilic enrichments of Candidatus Syntrophosphaera thermopropionivorans, a novel representative of the candidate phylum Cloacimonetes. In enrichment culture, methane was produced from propionate, while Ca. S. thermopropionivorans contributed 63% to total bacterial cells. The draft genome of Ca. S. thermopropionivorans encodes genes for propionate oxidation via methymalonyl‐CoA. Phylogenetically, Ca. S. thermopropionivorans affiliates with the uncultured Cloacimonadaceae W5 and is more distantly related (86.4% 16S rRNA gene identity) to Ca. Cloacimonas acidaminovorans. Although Ca. S. thermopropionivorans was enriched from a thermophilic biogas reactor, Ca. Syntrophosphaera was in particular associated with mesophilic anaerobic digestion systems. 16S rRNA gene amplicon sequencng and a novel genus‐specific quantitative PCR assay consistently identified Ca. Syntrophosphaera/Cloacimonadaceae W5 in 9 of 12 tested full‐scale biogas reactors thereby outnumbering other SPOB such as Pelotomaculum, Smithella and Syntrophobacter. Taken together the ubiquity and abundance of Ca. Syntrophosphaera, those SPOB might be key players for syntrophic propionate metabolism that have been overlooked before.

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Section: Genome Properties and Mechanisms For Syntrophysupporting

confidence: 87%

Candidatus Syntrophosphaera thermopropionivorans: a novel player in syntrophic propionate oxidation during anaerobic digestion

Dyksma

Gallert

2019

Environ Microbiol Rep

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“…Propionate is a key intermediate of anaerobic digestion in general and of biowaste in particular [ 1 , 2 , 3 , 4 , 5 , 6 , 7 ]. Main sources of propionate in bioreactors are odd numbered fatty acids from lipolysis of fat and oil as well as from carbohydrate and amino acid degradation [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

“…Main sources of propionate in bioreactors are odd numbered fatty acids from lipolysis of fat and oil as well as from carbohydrate and amino acid degradation [ 3 ]. Propionate accumulates during disturbances of the anaerobic digestion process, caused by e.g., toxic substances, high dry matter content, or high OLR close to overload, when hydrolysis, acidogenesis, acetogenesis, and methanogenesis, leading to biogas formation from complex organic matter, are no longer balanced [ 4 , 7 , 8 ]. Degradation of accumulated fatty acids such as propionate, however, is thermodynamically unfavorable [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

“…Degradation of propionate under strictly anaerobic conditions requires a hydrogen partial pressure of <6.5 Pa in a narrow thermodynamically defined window. Thus, in many anaerobic digesters high propionate concentrations can persist for a long time without failure of methane production, e.g., after start up or at high load conditions [ 1 , 4 , 7 ]. Deltaproteobacteria of the genera Syntrophobacter and Smithella , as well as Firmicutes belonging to the genera Pelotomaculum and Desulfotomaculum , are known to degrade propionate [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

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Microbial Community Shifts during Biogas Production from Biowaste and/or Propionate

Moertelmaier

Winter

et al. 2015

Bioengineering

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Propionate is the most delicate intermediate during anaerobic digestion as its degradation is thermodynamically unfavorable. To determine its maximum possible degradation rates during anaerobic digestion, a reactor was fed Monday to Friday with an organic loading rate (OLR) of 12/14 kg CODbiowaste·m−3·d−1 plus propionate up to a final OLR of 18 kg COD·m−3·d−1. No feed was supplied on weekends as it was the case in full-scale. To maintain permanently high propionate oxidizing activity (POA), a basic OLR of 3 kg CODpropionate·m−3·d−1 all week + 11 kg CODbiowaste·m−3·d−1 from Monday to Friday was supplied. Finally a reactor was operated with an OLR of 12 kg CODbiowaste·m−3·d−1 from Monday to Friday and 5 kg CODpropionate·m−3·d−1 from Friday night to Monday morning to maintain a constant gas production for permanent operation of a gas engine. The propionate degradation rates (PDRs) were determined for biowaste + propionate feeding. Decreasing PDRs during starvation were analyzed. The POA was higher after propionate supply than after biowaste feeding and decreased faster during starvation of a propionate-fed rather than a biowaste-fed inoculum. Shifts of the propionate-oxidizing and methanogenic community were determined.

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“…The fiber-like Methanosaeta cells are particularly advantageous in the formation of granules, hence their important role in wastewater treatment (31,32). Previously, we have found that M. harundinacea 6Ac undergoes a quorum sensing-regulated cell morphology transit from short to long filaments, and the former channels more acetate to cell biomass synthesis, while the latter directs more into methane production (9).…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic and Physiological Insights into the Robustness of Long Filamentous Cells of Methanosaeta harundinacea, Prevalent in Upflow Anaerobic Sludge Blanket Granules

Zhou

et al. 2015

Appl Environ Microbiol

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bMethanosaeta spp. are widely distributed in natural environments, and their filamentous cells contribute significantly to sludge granulation and the good performance of anaerobic reactors. A previous study indicated that Methanosaeta harundinacea 6Ac displays a quorum sensing-regulated morphological transition from short to long filaments, and more acetate is channeled into methane production in long filaments, whereas more is channeled into biomass synthesis in short filaments. Here, we performed transcriptomic and physiological analysis to gain insights into active methanogenesis in long filaments of M. harundinacea 6Ac. Both RNA sequencing (RNA-seq) and quantitative reverse transcription-PCR indicated that transcription of the genes involved in aceticlastic methanogenesis and energy metabolism was upregulated 1.2-to 10.3-fold in long filaments, while transcription of the genes for the methyl oxidative shunt was upregulated in short filaments. [2-13 C]acetate trace experiments demonstrated that a relatively higher portion of the acetate methyl group was oxidized to CO 2 in short filaments than in long filaments. The long filaments exhibited higher catalase activity and oxygen tolerance than the short ones, which is consistent with increased transcription of the oxidant-scavenging genes. Moreover, transcription of genes for cell surface structures was upregulated in the long filaments, and transmission electron microscopy revealed a thicker cell envelope in the filaments. RNA-seq determined a >2-fold upregulation of a variety of antistress genes in short filaments, like those encoding chaperones and DNA repair systems, which implies that the short filaments can be stressed. This study reveals the genetic basis for the prevalence of the long filamentous morphology of M. harundinacea cells in upflow anaerobic sludge blanket granules. Methanogenic degradation of organic complexes is a widely used approach in wastewater treatment; it promotes the development of upflow anaerobic sludge blanket (UASB) reactors (1). Efficient mineralization of organic complexes to CH 4 and CO 2 in the UASB reactor is implemented by diverse microbes through a food chain mode, where methanogens implement the final chemical reaction producing CH 4 (2, 3). In UASB reactors and other ecosystems, acetate-derived methane (aceticlastic methanogenesis) contributes about 70% of the methane produced by either the generalist aceticlastic methanogens of the species Methanosarcina or the obligate aceticlastic methanogens of the species Methanosaeta (4, 5). Methanosaeta species are believed to be the key components in anaerobic digesters not only because of their ability to use very low concentrations of acetate (threshold, 5 to 20 M) (6) but also because of their fiber-like cells that serve as a scaffold for the attachment of other organisms to promote the formation of UASB granules (7), an essential self-immobilized organization of the functional microbes. It has been reported that Methanosaeta cells comprise one-third of the anaerobic migrating ...

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Fatty acid metabolism and population dynamics in a wet biowaste digester during re-start after revision

Cited by 22 publications

References 23 publications

Candidatus Syntrophosphaera thermopropionivorans: a novel player in syntrophic propionate oxidation during anaerobic digestion

Candidatus Syntrophosphaera thermopropionivorans: a novel player in syntrophic propionate oxidation during anaerobic digestion

Microbial Community Shifts during Biogas Production from Biowaste and/or Propionate

Transcriptomic and Physiological Insights into the Robustness of Long Filamentous Cells of Methanosaeta harundinacea, Prevalent in Upflow Anaerobic Sludge Blanket Granules

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