Fatty Acid Supplementation Affects Skin Wound Healing in a Rat Model

Hokynková, Alica; Marie, Nováková; Babula, Petr; Sedláčková, Miroslava; Paulová, Hana; Hlaváčová, Miroslava; Charwátová, Daniela; Stračina, Tibor

doi:10.3390/nu14112245

Cited by 8 publications

(4 citation statements)

References 33 publications

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“…Similarly, studies investigating the effect of oleic acid on inflammation have put in evidence an anti-inflammatory effect induced by an increase in IL10 levels and a reduction in COX-2 levels [28]. This is in line with the improvement of skin wound healing that was observed in rats following supplementation with fatty acids [49] and with findings of topical oleic acid anti-inflammatory activity [50]. However, other authors have found a high expression of pro-inflammatory TNF and IL-17 in oleic acid-treated wounds [28].…”

Section: Thiobarbituric Acid (Tba) Assay Resultsmentioning

confidence: 55%

Nanoemulsions of Clove Oil Stabilized with Chitosan Oleate—Antioxidant and Wound-Healing Activity

et al. 2023

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Clove oil (CO) is a powerful antioxidant essential oil (EO) with anti-inflammatory, anesthetic, and anti-infective properties. It can be therefore considered a good candidate for wound-healing applications, especially for chronic or diabetic wounds or burns, where the balance of reactive oxygen species (ROS) production and detoxification is altered. However, EOs require suitable formulations to be efficiently administered in moist wound environments. Chitosan hydrophobically modified by an ionic interaction with oleic acid (chitosan oleate, CSO) was used in the present work to stabilize CO nanoemulsions (NEs). The dimensions of the NE were maintained at around 300 nm as the volume distribution for up to six months, and the CO content did not decrease to under 80% over 4 months, confirming the good stabilizing properties of CSO. The antioxidant properties of the CO NE were evaluated in vitro by a 2,2-diphenil-2-picrylhydrazyl hydrate (DPPH) assay, and in fibroblast cell lines by electron paramagnetic resonance (EPR) using α-phenyl-N-tert-butyl nitrone (PBN) as a spin trap; a protective effect was obtained comparable to that obtained with α-tocopherol treatment. In a murine burn model, the ability of CO formulations to favor macroscopic wound closure was evidenced, and a histological analysis revealed a positive effect of the CO NE on the reparation of the lesion after 18 days. Samples of wounds at 7 days were subjected to a histological analysis and parallel dosage of lipid peroxidation by means of a thiobarbituric acid-reactive substances (TBARS) assay, confirming the antioxidant and anti-inflammatory activity of the CO NE.

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Section: Thiobarbituric Acid (Tba) Assay Resultsmentioning

confidence: 55%

Nanoemulsions of Clove Oil Stabilized with Chitosan Oleate—Antioxidant and Wound-Healing Activity

et al. 2023

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“…In general, the literature suggests that the anti-inflammatory action of ω-3 PUFA requires a minimal administration period of 14 days, and that the prophylactic supplementation for the same period increases EPA and DHA levels in the cell membrane. Consequently, the cell membrane becomes robust and resistant to lysis [ 27 , 31 , 32 ].…”

Section: Discussionmentioning

confidence: 99%

A diet rich in omega-3 fatty acid improves periodontitis and tissue destruction by MMP2- and MMP9-linked inflammation in a murine model

et al. 2023

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Periodontitis is an oral-cavity inflammatory disease and is the principal cause associated with tooth loss. Matrix metalloproteinases 2 and 9 (MMP-2 and MMP-9) are important proteases involved in periodontal tissue destruction. The omega-3 polyunsaturated fatty acids (ω-3 PUFA) have been demonstrated to possess immunoregulatory properties in periodontitis. The aim of the study was to investigate the effects of ω-3 PUFA on inflammation and on the expression of MMP-2 and -9 in a murine periodontitis model. Twenty-four male C57BL/6 mice were divided into control mice (Control), control mice treated with ω-3 PUFA (O3), mice with periodontitis (P), and mice with periodontitis treated with ω-3 PUFA (P + O3). ω-3 PUFA were administered orally once a day for 70 days. Periodontitis in mice was induced by Porphyromonas gingivalis-infected ligature placement around the second maxillary molar. The mice were sacrificed, and blood and maxillary samples were collected. Flow cytometry was used to quantify tumor necrosis factor-alpha (TNFα), interleukin (IL)-2, IL-4, IL-5, and interferon-gamma. Histologic analysis and immunohistochemistry for MMP-2 and -9 were performed. The data were statistically evaluated using analysis of variance (ANOVA) and the Tukey post hoc test. Histological analysis showed that ω-3 PUFA supplementation prevented inflammation and tissue destruction and revealed that bone destruction was more extensive in the P group than in the P + O3 group (p < 0.05). Also, it decreased the serum expressions of TNFα and IL-2 and the tissue expression of MMP-2 and -9 in the periodontitis-induced model (p < 0.05). ω-3 PUFA supplementation prevented alveolar bone loss and periodontal destruction, probably by decreasing the expression of MMP-2 and MMP-9 and its immunoregulatory properties.

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“…Polyunsaturated fatty acids (PUFAs), which mainly comprise omega-3 and omega-6 fatty acids, can regulate the immune response and participate in skin wound healing, repair, and tissue remodelling [ 58–60 ]. In diabetic rat skin wound healing models, omega-3 PUFAs have been found to alter proinflammatory cytokine production, which substantially decreased the number of grade three mast cells on days 3 and 5 and the wound area by day 7 [ 61 ].…”

Section: Reviewmentioning

confidence: 99%

Metabolic reprogramming in skin wound healing

Wang,

Zhao,

et al. 2024

Burns &Amp; Trauma

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Metabolic reprogramming refers to the ability of a cell to alter its metabolism in response to different stimuli and forms of pressure. It helps cells resist external stress and provides them with new functions. Skin wound healing involves the metabolic reprogramming of nutrients, such as glucose, lipids, and amino acids, which play vital roles in the proliferation, differentiation, and migration of multiple cell types. During the glucose metabolic process in wounds, glucose transporters and key enzymes cause elevated metabolite levels. Glucose-mediated oxidative stress drives the proinflammatory response and promotes wound healing. Reprogramming lipid metabolism increases the number of fibroblasts and decreases the number of macrophages. It enhances local neovascularization and improves fibrin stability to promote extracellular matrix remodelling, accelerates wound healing, and reduces scar formation. Reprogramming amino acid metabolism affects wound re-epithelialization, collagen deposition, and angiogenesis. However, comprehensive reviews on the role of metabolic reprogramming in skin wound healing are lacking. Therefore, we have systematically reviewed the metabolic reprogramming of glucose, lipids, and amino acids during skin wound healing. Notably, we identified their targets with potential therapeutic value and elucidated their mechanisms of action.

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Fatty Acid Supplementation Affects Skin Wound Healing in a Rat Model

Cited by 8 publications

References 33 publications

Nanoemulsions of Clove Oil Stabilized with Chitosan Oleate—Antioxidant and Wound-Healing Activity

Nanoemulsions of Clove Oil Stabilized with Chitosan Oleate—Antioxidant and Wound-Healing Activity

A diet rich in omega-3 fatty acid improves periodontitis and tissue destruction by MMP2- and MMP9-linked inflammation in a murine model

Metabolic reprogramming in skin wound healing

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