Feasibility of a nested PCR for the diagnosis of Trichomonas vaginalis infection in women a Wasit Province, Iraq

Abstract

This study was concerned the describe of an current PCR method for specific detection of Tichomonas vaginalis DNA into a rapid nested PCR assay, and to estimate the recent prevalence of T. vaginalis according to studying different factors that effect on vaginal trichomoniasis in women complaining of vaginal discharge. Vaginal swab samples were collected from 100 patients lived at Wasit province, during the period from January, 2022 to July, 2022. Demographic data, clinical and socioeconomic status were collected from the patients using a structured questionnaire. Total genomic DNA was isolated from 100 samples of vaginal swab randomly chosen to specific detection of T. vaginalis DNA into a rapid nested PCR. All samples were examined by the direct microscopic examination (wet mount). The vaginal swabs examined by wet mount and nested PCR to detect the presence of T. vaginalis. Of the 100 women, 29 were positive by nested PCR giving the prevalence rate of (29%) with and the direct microscopic examination (wet mount) showed 4/100 (4 %) positive case. Their ages were different. Women aged (20-30)years had significantly higher prevalence of trichomoniasis (14%) than other age groups.

Systemic Review of Trichomonas Vaginalis in Iraq

“…There were some similarities in results between different governorates of Iraq. The prevalence of T. vaginalis in Baqubah was 54.1% during 2017 [17], it was similar to that recorded in Babil 2015 and Basra 2013 which were 50%, 55.4% [18,19], also there was similarity in the result that recorded in the same year 2013 in Kut [20] and Kirkuk [21] which were 20% and 20.49% respectively. Data analysis during 2014 and 2017 from two different governorate reveals similar result which were 21.76% and 17.64% from Kut and Njaf [22,23].…”