Feasibility of spent metalworking fluids as co-substrate for anaerobic co-digestion

Rodríguez-Verde, Iván; Regueiro, Leticia; Pena, Rocío; Álvarez, J.A.; Lema, Juan M.; Carballa, Marta

doi:10.1016/j.biortech.2013.12.090

Cited by 18 publications

(12 citation statements)

References 28 publications

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“…However, in general the aerobic process, as compared to the anaerobic one, resulted in better COD removal rates and higher contribution of biodegradation mechanism [14,50]. It thus appears that the anaerobic treatment technology requires further optimization actions such as supplementation with complementary physical-chemical processes, addition of co-substrates and/or extensive bioaugmentation [50,51].…”

Section: Discussionmentioning

confidence: 99%

Bioremediation Of A Spent Metalworking Fluid With Auto- And Allochthonous Bacterial Consortia

Kaszycki

Petryszak

Supel

2015

Ecological Chemistry and Engineering S

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Spent mineral oil-based metalworking fluids are waste products of the machining processes and contribute substantially to the global industrial pollution with petroleum oil products. Wastewaters containing oily emulsions are ecologically hazardous and thus a variety of methods have been implemented to prevent these effluents from affecting the natural environment. Most of these methods rely upon physical-chemical treatment and phase separation; however, none of them proved to be effective enough to meet tightening environmental regulations. Therefore, novel technologies need to be elaborated and there is growing interest in implementing biological treatment methods based on microbial bioremediation. In this study an oil/water emulsion obtained from a waste stream of the metal-processing industry was tested for biodegradability of its organic constituents. This liquid waste was found non-toxic to bacterial consortia and was colonized with indigenous microorganisms (approx. 10 7 cfu · cm -3). The total load of organic content was determined as a chemical oxygen demand (COD) value of 48 200 mg O2 · dm -3. Emulsion treatment was carried out using a threefold wastewater dilution and employing two variants of biostimulated aerobic bacterial communities: (1) uninoculated emulsion, where bioremediation was carried out by the autochthonous bacteria alone, and (2) wastewater samples inoculated with a ZB-01 microbial consortium which served as a source of specialized bacteria for process bioaugmentation. Biodegradation efficiency achieved in a 14-day test was monitored by measuring both the COD parameter and the concentration of high-boiling organic compounds. Both approaches yielded satisfactory results showing significant reduction of the emulsion organic fraction; however, the resultant decrease of wastewater load tended to be more efficient for the case where the process was bioaugmented with the inoculated consortium. Gas chromatography analyses coupled with mass spectrometric detection (GC-MS) confirmed high degradation yields obtained for both cases studied (58 and 71%, respectively) in a 28-day test. It is concluded that oil-based metalworking emulsions can undergo efficient biological treatment under conditions enabling aerobic bacterial proliferation and that xenobiotic biodegradation kinetics can be accelerated by bioaugmenting the process with allochthonous microbial consortia.

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Section: Discussionmentioning

confidence: 99%

Bioremediation Of A Spent Metalworking Fluid With Auto- And Allochthonous Bacterial Consortia

Kaszycki

Petryszak

Supel

2015

Ecological Chemistry and Engineering S

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“…Jagadevan et al (2011) reported 65% COD removal following fenton processing of MWFs, and 92% COD removal with biological treatment following fenton processing. Rodriguez-Verde et al (2014) published a 74% COD removal using pig manure as co-substrate in an anaerobic digester and biogas production of 0.26 L/L day at 0.7 g COD/L day OLR. Batch studies have tested kinetic models of anaerobic treatments in wastewaters from vinasse (Kobya and Demircioglu, 1993;Filikİsç en, 2006), textiles (Gnanapragasam et al, 2011), whey (Ergüder et al, 2000), sunflower meal (Raposo et al, 2009), apples (Kafle and Kim, 2013), corn (Raposo et al, 2006), slaughterhouses (Slimane et al, 2014), and dyes (Hosseini Koupaie, 2012).…”

Section: Introductionmentioning

confidence: 99%

Treatment of automotive industry wastewater using anaerobic batch reactors: The influence of substrate/inoculum and molasses/wastewater

Sarıoğlu

Gökçek

2016

Process Safety and Environmental Protection

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“…Conversely, Rodriguez‐Verde et al . () examined the anaerobic digestion of spent MWF by testing the BMP and found 25% methane production with a yield of 15.6 l CH 4 per kg of spent MWF. They did, however, increase the methane production up to 39% when the spent MWF was mixed with pig manure.…”

Section: Introductionmentioning

confidence: 99%

“…Over an operating period of 92 days, at an OLR of 11.9-51.3 kgCOD m À3 day À1 they achieved 67.1% COD and 71.3% TOC removal; however, the volumetric rate of biogas formation was very difficult to assess due to the small amounts that were generated. Conversely, Rodriguez-Verde et al (2014) examined the anaerobic digestion of spent MWF by testing the BMP and found 25% methane production with a yield of 15.6 l CH 4 per kg of spent MWF. They did, however, increase the methane production up to 39% when the spent MWF was mixed with pig manure.…”

Section: Introductionmentioning

confidence: 99%

“…Previous anaerobic bioreactor studies have largely used inocula of undefined microbial communities from activated or anaerobic sludge (Perez et al, 2006;Rodriguez-Verde et al, 2014;Teli et al, 2015). However, the antimicrobial nature of MWFs is invariably too hostile for such inocula, and especially to methanogens as was found in BMP and ATA tests by Teli et al, 2015. Carefully, constructed consortia of MWF resistant anaerobic bacteria may improve the opportunity to establish treatment systems.…”

Section: Introductionmentioning

confidence: 99%

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Selection and assembly of indigenous bacteria and methanogens from spent metalworking fluids and their potential as a starting culture in a fluidized bed reactor

Vyrides

Rivett

Bruce

et al. 2019

Microbial Biotechnology

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Summary Waste metalworking fluids (MWFs) are highly biocidal resulting in real difficulties in the, otherwise favoured, bioremediation of these high chemical oxygen deman (COD) wastes anaerobically in bioreactors. We have shown, as a proof of concept, that it is possible to establish an anaerobic starter culture using strains isolated from spent MWFs which are capable of reducing COD or, most significantly, methanogenesis in this biocidal waste stream. Bacterial strains (n = 99) and archaeal methanogens (n = 28) were isolated from spent MWFs. The most common bacterial strains were Clostridium species (n = 45). All methanogens were identified as Methanosarcina mazei. Using a random partitions design (RPD) mesocosm experiment, we found that bacterial diversity and species–species interactions had significant effects on COD reduction but that bacterial composition did not. The RPD study showed similar effects on methanogenesis, except that composition was also significant. We identified bacterial species with positive and negative effects on methane production. A consortium of 16 bacterial species and three methanogens was used to initiate a fluidized bed bioreactor (FBR), in batch mode. COD reduction and methane production were variable, and the reactor was oscillated between continuous and batch feeds. In both microcosm and FBR experiments, periodic inconsistencies in bacterial reduction in fermentative products to formic and acetic acids were identified as a key issue.

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Feasibility of spent metalworking fluids as co-substrate for anaerobic co-digestion

Cited by 18 publications

References 28 publications

Bioremediation Of A Spent Metalworking Fluid With Auto- And Allochthonous Bacterial Consortia

Bioremediation Of A Spent Metalworking Fluid With Auto- And Allochthonous Bacterial Consortia

Treatment of automotive industry wastewater using anaerobic batch reactors: The influence of substrate/inoculum and molasses/wastewater

Selection and assembly of indigenous bacteria and methanogens from spent metalworking fluids and their potential as a starting culture in a fluidized bed reactor

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