Feasibility of Thin Layer Chromatography as an Inexpensive Alternative to Gas‐Liquid Chromatography for the Identification of Some Anaerobic Gram Positive Non‐sporing Rods

Abstract

Thin‐layer chromatography (TLC) was employed as an alternative to gas‐liquid chromatography (GLC) for the analysis of typical fermentation end‐products of anaerobic Gram positive non‐sporing bacteria. The inexpensive TLC method succeeded in differentiating Actinomyces, Bifidobacterium, Lactobacillus and Propionibacterium. Ether extraction of supernatant was found to be unnecessary; volatile and non‐volatile acids were separated simultaneously, with consequent saving of time.

The Detection of Microbial Spoilage in Canned Foods Using Thin‐layer Chromatography

“…It is also possible to detect these acids by using thin-layer chromatography (t.1.c.) (Ganshirt et al 1962;Palasuntherhm et al 1977). A suitable t.1.c.…”