Feasibility of Xpert Ebola Assay in Médecins Sans Frontières Ebola Program, Guinea

Bergh, Rafaël Van den; Chaillet, Pascale; Sow, Mamadou; Amand, Mathieu; Vyve, Charlotte van; Jonckheere, Sylvie; Crestani, Rosa; Sprecher, Armand; Herp, Michel Van; Chua, Arlene; Piriou, Erwan; Koivogui, Lamine; Antierens, Annick

doi:10.3201/eid2202.151238

Cited by 23 publications

(22 citation statements)

References 7 publications

(7 reference statements)

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“…87,117,118 GeneXpert has been deployed successfully to the point-of-care at Ebola treatment centers during the recent outbreak in west Africa. 88,89 The GeneXpert has been shown to have a low tolerance for heat and humidity 119 ; improvements to increase robustness and decrease maintenance requirements will allow more widespread adoption. A competing platform to GeneXpert, BioFire FilmArray (bioMérieux, Marcy l'Etoile, France), uses cartridges to simultaneously test for multiple pathogens within a syndrome, ranging from diarrhea to meningoencephalitis.…”

Section: Traditional Microbiology Techniques Reinvent Themselvesmentioning

confidence: 99%

Reducing Uncertainty for Acute Febrile Illness in Resource-Limited Settings: The Current Diagnostic Landscape

Robinson

Manabe

2017

The American Society of Tropical Medicine and Hygiene

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Diagnosing the cause of acute febrile illness in resource-limited settings is important-to give the correct antimicrobials to patients who need them, to prevent unnecessary antimicrobial use, to detect emerging infectious diseases early, and to guide vaccine deployment. A variety of approaches are yielding more rapid and accurate tests that can detect more pathogens in a wider variety of settings. After decades of slow progress in diagnostics for acute febrile illness in resource-limited settings, a wave of converging advancements will enable clinicians in resource-limited settings to reduce uncertainty for the diagnosis of acute febrile illness.

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Section: Traditional Microbiology Techniques Reinvent Themselvesmentioning

confidence: 99%

Reducing Uncertainty for Acute Febrile Illness in Resource-Limited Settings: The Current Diagnostic Landscape

Robinson

Manabe

2017

The American Society of Tropical Medicine and Hygiene

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“…Of note, a small fraction of specimens failed Xpert testing due to endogenous/exogenous internal control failure or system failure, highlighting both the need for adequate sample and reagent integrity and the potential requirement for technical expertise for troubleshooting and equipment maintenance. The Xpert Ebola assay performed similarly well in a study carried out at an Ebola treatment center near Conakry, Guinea, showing 100% sensitivity with 96.0% specificity in fresh venipuncture whole-blood specimens (n ϭ 218; 26 Ebola virus-positive specimens) compared to an in-house RT-PCR assay (NP gene target) used for routine clinical diagnostic testing at the national reference laboratory at Gamal Abdel Nasser University of Conakry, located near the treatment center (75). All discordant specimens (benchmark RT-PCR negative/Xpert positive) were obtained from known EVD patients undergoing monitoring for viral clearance, again suggesting a higher actual specificity for the Xpert assay and highlighting the performance of this assay in the convalescent stage of EVD.…”

Section: Evd Diagnostic Tests With Emergency Use Authorizationmentioning

confidence: 90%

Diagnosis of Ebola Virus Disease: Past, Present, and Future

2016

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SUMMARYLaboratory diagnosis of Ebola virus disease plays a critical role in outbreak response efforts; however, establishing safe and expeditious testing strategies for this high-biosafety-level pathogen in resource-poor environments remains extremely challenging. Since the discovery of Ebola virus in 1976 via traditional viral culture techniques and electron microscopy, diagnostic methodologies have trended toward faster, more accurate molecular assays. Importantly, technological advances have been paired with increasing efforts to support decentralized diagnostic testing capacity that can be deployed at or near the point of patient care. The unprecedented scope of the 2014-2015 West Africa Ebola epidemic spurred tremendous innovation in this arena, and a variety of new diagnostic platforms that have the potential both to immediately improve ongoing surveillance efforts in West Africa and to transform future outbreak responses have reached the field. In this review, we describe the evolution of Ebola virus disease diagnostic testing and efforts to deploy field diagnostic laboratories in prior outbreaks. We then explore the diagnostic challenges pervading the 2014-2015 epidemic and provide a comprehensive examination of novel diagnostic tests that are likely to address some of these challenges moving forward.

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“…Urinalysis was performed with Combi 10L strips (Macherey-Nagel, Germany) upon request from clinicians. A field evaluation of the GeneXpert as primary diagnosis tool for EVD was conducted from weeks 19 to 28 at the Donka ETC, 4 after which the analyzer was implemented as part of the screening process for admission to both ETCs.…”

Section: Laboratory Testing Setupmentioning

confidence: 99%

Ensuring On-site Ebola Patient Monitoring and Follow-up: Development of a Laboratory Structure Embedded in an Ebola Treatment Center

Williams¹,

Amand

Bergh³

et al. 2019

Disaster med. public health prep.

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The capacity to rapidly distinguish Ebola virus disease from other infectious diseases and to monitor biochemistry and viremia levels is crucial to the clinical management of suspected Ebola virus disease cases. This article describes the design and practical considerations of a laboratory straddling the high- and low-risk zones of an Ebola treatment center to produce timely diagnostic and clinical results for informed case management of Ebola virus disease in real-life conditions. This innovation may be of relevance for actors requiring flexible laboratory implementation in contexts of high-communicability, high-lethality disease outbreaks.

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Feasibility of Xpert Ebola Assay in Médecins Sans Frontières Ebola Program, Guinea

Abstract: This assay provides results in less time than routine PCR and is equally sensitive.

Cited by 23 publications

References 7 publications

Reducing Uncertainty for Acute Febrile Illness in Resource-Limited Settings: The Current Diagnostic Landscape

Reducing Uncertainty for Acute Febrile Illness in Resource-Limited Settings: The Current Diagnostic Landscape

Diagnosis of Ebola Virus Disease: Past, Present, and Future

Ensuring On-site Ebola Patient Monitoring and Follow-up: Development of a Laboratory Structure Embedded in an Ebola Treatment Center

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