Features of immune status in different states of uric acid metabolism in female rats

Abstract

zukow@umk.pl 4 OO Bohomolets' Institute of Physiology, Kyїv i.popovych@biph.kiev.ua AbstractBackground. Previously, we found a wide range of uric acid metabolism parameters and functional relationships of uricemia and uricosuria with the parameters of immunity in healthy female rats analyzed. It was found by canonical correlation analysis that both parameters of uric acid metabolism determine the immunity status of rats by 71%. The purpose of this study is to determine the features of immune status in rats with quantitatively and qualitatively different uric acid metabolism. Material and Methods. Experiment was performed on 60 healthy female Wistar rats 220-300 g. Among them 10 animals remained intact, using tap water from drinking ad libitum. The rats of others groups for 6 days administered through the tube various fluids. The serum and urine levels of the uric acid (uricase method) were determined. In the blood, the parameters of immunity were determined. From thymus and spleen made smears-imprints for counting splenocytogram and thymocytogram. For them, as well as immunocytogram and leukocytogram of blood, Shannon's entropy was calculated. Results. Screening of immune profiles of four quantitative and qualitative uric acid metabolism clusters revealed 6 functionalmetabolic patterns, two of which are quasilinear (enhancing and suppressing), with immune extremes at moderately elevated uricemia, two U-shaped with immune optimum at moderately reduced uricemia levels, as well as a non-reactive pattern. Discriminant analysis revealed 14 parameters of immunity, the constellation of which four clusters of metabolism of uric acid clearly distinguish. Conclusion. Endogenous uric acid exerts a modulatory immunotropic effect in healthy female rats.168

Immunotropic effects of so-called slag metabolites (creatinine, urea, uric acid and bilirubin) at rats

“…The study of the effect of end products of protein and nucleic acid metabolism on the immune system is of interest not only theoretical but also practical in line with the problems of urotoxins and hemodialysis. In previous studies, we found significant links between uricemia and uricosuria, on the one hand, and immune parameters, on the other hand, in both healthy rats [17,18] and patients in Truskavets' spa [19,20,37]. In this experimental study, the spectrum of nitrogenous metabolites was expanded due to creatinine, urea and bilirubin.…”

Relationships between changes in uric acid parameters metabolism and parameters of immunity and microbiota in patients with neuroendocrine-immune complex dysfunction

“…Previously, continuing experimental studies [6][7][8]10], by screening correlation links between uricemia and uricosuria, on the one hand, and immunity and microbiota parameters, on the other, registered in each of 44 patients with chronic pyelonephritis and cholecystitis in remission twice (before and after ten days of balneotherapy), we found that raw uricemia determines by 28% nine parameters of immunity as well as bacteriuria and content in E. coli feces. Uricemia, normalized by sex and age, determines by 25% another constellation of immunity parameters as well as content in E. coli feces with impaired enzymatic activity and Klebsiela&Proteus.…”

Relationships between parameters of uric acid exchange and immunity as well as microbiota in patients with neuroendocrine-immune complex dysfunction

“…In the above experiment in rats [8][9][10], we found that Uric acid level correlated positively with the content in the thymus of Lymphocytes and Macrophages, but negatively correlated with the content of Epitheliocytes, Reticulocytes and Hassal's corpuscles. If these data are transferred to humans, it can be suggested that Uric acid stimulates the differentiation of thymocytes to CD8 + T-cells, instead reducing the formation of CD4 + T-cells.…”

“…Previously, we found a wide range of uric acid exchange parameters grouped into four clusters [7] and functional relationships of uricemia and uricosuria with the parameters of immunity in healthy rats analyzed [8][9][10].…”