Fed-batch versus batch cultures of Yarrowia lipolytica for γ-decalactone production from methyl ricinoleate

Gomes, Nelma; Teixeira, J. A.; Belo, Isabel

doi:10.1007/s10529-011-0824-0

Cited by 54 publications

(34 citation statements)

References 18 publications

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“…The concentration, yield, and productivity achieved in the present study using permeabilized cells of W. lipofer were 11.1-fold, 52%, and 7.9-fold higher than the highest previously observed values for ␥-dodecalactone production. Recently, ␥-decalactone productivity for batch cultures of Y. lipolytica was observed to be 168 mg/liter/h using 30 g/liter of methyl ricinoleate (36). Y. lipolytica produced 12.3 g/liter of ␥-decalactone from 60 g/liter of castor oil with a conversion yield of 21% and a productivity of 240 mg/liter/h (37), which were previously the highest reported concentration and productivity in the production of flavor lactones.…”

Section: Resultsmentioning

confidence: 99%

New Biotransformation Process for Production of the Fragrant Compound γ-Dodecalactone from 10-Hydroxystearate by Permeabilized Waltomyces lipofer Cells

Joo

2013

Appl Environ Microbiol

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A new biotransformation process for the production of the flavor lactone was developed by using permeabilized Waltomyces lipofer, which was selected as an efficient ␥-dodecalactone-producing yeast among 10 oleaginous yeast strains. The optimal reaction conditions for ␥-dodecalactone production by permeabilized W. lipofer cells were pH 6.5, 35°C, 200 rpm, 0.7 M Tris, 60 g/liter of 10-hydroxystearic acid, and 30 g/liter of cells. Under these conditions, nonpermeabilized cells produced 12 g/liter of ␥-dodecalactone after 30 h, with a conversion yield of 21% (wt/wt) and a productivity of 0.4 g/liter/h, whereas permeabilized cells obtained after sequential treatments with 50% ethanol and 0.5% Triton X-100 produced 46 g/liter of ␥-dodecalactone after 30 h, with a conversion yield of 76% (wt/wt) and a productivity of 1.5 g/liter/h. These values were 3.7-and 3.8-fold higher than those obtained using nonpermeabilized cells. These are the highest reported concentration, conversion yield, and productivity for the production of the bioflavor lactone.

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Section: Resultsmentioning

confidence: 99%

New Biotransformation Process for Production of the Fragrant Compound γ-Dodecalactone from 10-Hydroxystearate by Permeabilized Waltomyces lipofer Cells

Joo

2013

Appl Environ Microbiol

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“…g-Decalactone is typically produced using methyl ricinoleate derived from castor oil as substrate (Gomes et al, 2012;Guo et al, 2012;Rabenhorst & Gatfield, 2002;Schrader et al, 2004).…”

Section: Industrial Uses Of Y Lipolytica: Past Present and Futurementioning

confidence: 99%

Yarrowia lipolytica: Safety assessment of an oleaginous yeast with a great industrial potential

Groenewald

Boekhout

Neuvéglise

et al. 2013

Critical Reviews in Microbiology

397

288

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Yarrowia lipolytica has been developed as a production host for a large variety of biotechnological applications. Efficacy and safety studies have demonstrated the safe use of Yarrowia-derived products containing significant proportions of Yarrowia biomass (as for DuPont's eicosapentaenoic acid-rich oil) or with the yeast itself as the final product (as for British Petroleum's single-cell protein product). The natural occurrence of the species in food, particularly cheese, other dairy products and meat, is a further argument supporting its safety. The species causes rare opportunistic infections in severely immunocompromised or otherwise seriously ill people with other underlying diseases or conditions. The infections can be treated effectively by the use of regular antifungal drugs, and in some cases even disappeared spontaneously. Based on our assessment, we conclude that Y. lipolytica is a ''safe-to-use'' organism.

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“…Sterile crude glycerol and castor oil were added separately in volume fractions of 10, 20 and 30 % (i.e. 5, 10 and 15 mL, respectively) (15,16). The pH value was adjusted to pH=6 using a digital pH meter (model B474; Micronal, São Paulo, Brazil) before the inoculation by the addition of NaOH (2 mol/L) and HCl (2 mol/L).…”

Section: γ-Decalactone Production and Analysismentioning

confidence: 99%

“…When comparing the production of γ-deca lactone and the cell population in the medium, using the same type of substrate and its volume fractions (Table 1), it was found that the highest yield was achieved with the highest number of cells, using both carbon sources. In general, castor oil is the most suitable substrate and is used for the yeast growth and production of γ-decalactone (15).…”

Section: Growth and γ-Decalactone Production Under Different Volume Fmentioning

confidence: 99%

Gamma-Decalactone Production by Yeast Strains under Different Conditions

Andrade

Carvalho

Schwan

et al. 2017

Food Technol. Biotechnol.

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Summaryγ-Decalactone is a flavour compound that when obtained by biotechnological production using microorganisms is classified as natural. The aim of this study is to evaluate various conditions for γ-decalactone production by tropical yeast strains Yarrowia lipolytica CCMA 0242 and Lindnera saturnus CCMA 0243. The growth of and γ-decalactone production by Y. lipolytica CCMA 0242 were higher in castor oil than in glycerol. γ-Decalactone production in single batch or fed-batch fermentation did not differ significantly. The γ-decalactone production by L. saturnus CCMA 0243 was better at initial pH=5, while the production by Y. lipolytica CCMA 0242 was better at initial pH=6. The yeast L. saturnus CCMA 0243 produced more γ-decalactone than Y. lipolytica CCMA 0242 under the same fermentation conditions. The crude glycerol was not an alternative substrate for γ-de ca lac tone production by Y. lipolytica CCMA 0242. Castor oil at volume fraction of 30 % showed better re sults as a substrate. The strain L. saturnus CCMA 0243 showed better results of γ-decalactone production. This yeast species can be considered an alternative producer of γ-decalactone in biotechnological processes.

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Fed-batch versus batch cultures of Yarrowia lipolytica for γ-decalactone production from methyl ricinoleate

Cited by 54 publications

References 18 publications

New Biotransformation Process for Production of the Fragrant Compound γ-Dodecalactone from 10-Hydroxystearate by Permeabilized Waltomyces lipofer Cells

New Biotransformation Process for Production of the Fragrant Compound γ-Dodecalactone from 10-Hydroxystearate by Permeabilized Waltomyces lipofer Cells

Yarrowia lipolytica: Safety assessment of an oleaginous yeast with a great industrial potential

Gamma-Decalactone Production by Yeast Strains under Different Conditions

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