The aim of this study was to explore the bus operating state of the city bus passenger corridor, taking the minimum bus operating cost and passenger travel cost as the objective function, taking passenger flow demand and operating income as the constraint, and considering the average speed change of the bus line in the bus corridor at different times. This paper proposes a dynamic optimization model of bus route schedule based on bus Integrated Circuit Card (IC Card) data. The optimization variable is the departure frequency of the candidate lines. To solve the model, a dynamic departure interval optimization method based on improved Genetic Algorithm (GA) was designed under different decision preferences. The method includes the calibration of generalized cost functions for passengers and bus companies and grasps the characteristics of bus operating speed changes and the design of departure strategies under different decision preferences. The validity and applicability of the proposed method are verified by a numerical example. We mainly carried out the following work: (1) Dynamic analysis of the time dimension of the bus departure interval takes into account the changes in passenger time characteristics during peak periods. (2) Seven schemes of weight ratio of passenger waiting time cost and bus operation cost were designed, and the departure intervals with different benefit orientations of passengers and operators were discussed, respectively, so as to select the corresponding departure schemes for decision makers under different decision preferences. The results show that (1) the total cost of the 7 different weighting schemes is lower than the actual value by 6.90% to 18.20%; (2) when decision makers need to bias the weight to the bus company, the weight ratio α : β between passengers and bus company is 0.25 : 0.75 which works best. The frequency of departures has been reduced by 6, and at the same time, the total optimized cost is reduced by 18.2%; (3) when decision makers need to bias the weight to the passengers, the weight ratio α : β between the passengers and bus company is 0.75 : 0.25 which works best. The frequency of departures has been increased by 19, and at the same time, the total optimized cost is reduced by 17.7%; and (4) when decision makers consider passengers and bus companies equally, the weight ratio α : β between passengers and bus companies is 0.5 : 0.5, the optimization cost is the closest to the actual cost, the optimization cost is reduced by 6.9%, and the frequency of departures has been increased by 5. The results show that the model in this paper provides a new idea for the information mining of bus routes in the research based on the bus IC Card data and provides an effective tool for the management of different operation decision preferences.