Feeding gelatinized carbohydrate in the diets of magur,Clarias batrachus(Linnaeus, 1758): Effects on growth performance, enzyme activities and expression of muscle regulatory factors

Abstract

This study was conducted to illustrate the effect of dietary gelatinized starch (GS) on the growth performance, enzyme activities and expression of MyoD and Myf5 in magur, Clarias batrachus fingerlings. Four iso-nitrogenous (37%) and iso-lipidic (6%) diets containing 15% (D-1), 25% (D-2), 35% (D-3) or 45% (D-4) GS were fed to 240 fingerlings (2.5 ± 0.5 g) in triplicates groups for 8 weeks. The maximum weight gain %, specific growth rate, protein efficiency ratio, lower FCR and higher mRNA expression of MyoD or Myf5 were found in the D-3 group fed with 35% GS. Higher hepatosomatic index, viscerosomatic index, body lipid and lower moisture content were found in the D-4 group. Aspartate transaminase and alanine transaminase activities were found to be higher in the D-1 group. Amylase, glucose-6-phosphate dehydrogenase and blood glucose were higher in the D-4 group. Superoxide dismutase, catalase and hexokinase activities remain unaffected by the dietary GS levels.Hence, the overall results indicate that 35% GS can improve growth performance and upregulate myogenic regulatory factors, but 45% GS level will favor lipogenesis and compromise growth. Furthermore, Myf5 gene showed more immediate response than the MyoD to the dietary carbohydrate in magur. K E Y W O R D SClarias batrachus, gelatinized starch, Myf5, MyoD, myogenic regulatory factors

Effects of dietary protein on growth performance, nutrient utilization, digestive enzymes and physiological status of grey mullet,Mugil cephalusL. fingerlings reared in inland saline water

“…A similar result has been reported in grey mullet (Abdel‐Hakim et al, ; De et al, ; Papaparaskeva‐Papoutsoglou & Alexis, ) and other species (Khattab, Ahmad, Shalaby, & Abdel‐Tawwab, ; Mohanta et al, ). Body indices are used to evaluate available energy in fish, and excess availability of energy leads to the deposition of glycogen and lipid (Talukdar et al, ). The highest HSI in the lower CP diet can be related to an imbalance in P/E ratio as excess carbohydrate is being converted to liver glycogen and lipid, and thus the weight of the liver increased (Yang et al, ).…”

Molecular adaptations of glucose and lipid metabolism to different levels of dietary carbohydrates in juvenile Japanese flounderParalichthys olivaceus

“…Moreover, exogenous glucose provides NADPH or carbon backbones for de novo lipogenesis in fish (Sargent, Tocher, & Bell, ). Indeed, dietary carbohydrate intake elevated activities of lipogenic enzymes and induced lipid accumulation in several fish species, such as Senegalese sole ( Solea senegalensis ) and magur ( Clarias batrachus ) (Dias et al, ; Talukdar et al, ). However, excessive dietary carbohydrates can have a negative impact on fish health and lead to metabolic disorders.…”

Dietary raw starch to gelatinized starch ratios: Effects on the growth performance, digestive functions, intestinal histology and growth hormone‐insulin‐like growth factor‐I axis of blunt snout breamMegalobrama amblycephala

“…However, this could be largely resolved with its structures and properties modified by certain processing methods such as pre-gelatinization and expansion (Tako, Tamaki, Teruya, & Takeda, 2014). Accordingly, several studies have proven that gelatinization can greatly improve the starch digestibility of fish (Gallego, Bazoco, Akharbach, Suárez, & Sanz, 1994;Peres & Oliva-Teles, 2002;Romano et al, 2018;Talukdar et al, 2019). However, the total replacement of RS by GS resulted in a remarkable growth retardation in several fish species like European sea bass (Dicentrarchus larax) (Peres & Oliva-Teles, 2002) and yellowfin seabream (Sparus latus) (Wu, Liu, Tian, Mai, & Yang, 2007).…”