Feeding restriction alleviates high carbohydrate diet-induced oxidative stress and inflammation of Megalobrama amblycephala by activating the AMPK-SIRT1 pathway

Xu, Chao; Liu, Wenbin; Remø, Sofie C.; Wang, Bingke; Shi, Haojie; Zhang, Li; Liu, Jia-Dai; Li, Xiangfei

doi:10.1016/j.fsi.2019.06.057

Cited by 42 publications

(18 citation statements)

References 85 publications

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“…In present study, the activity of antioxidant enzymes T-SOD, CAT and GPX and expression level of antioxidant-related genes (SOD1, SOD2, SOD3a, CAT and GPX) in H group decreased compared with L and M group, while the concentration of MDA increased signi cantly, indicating impairment to the intestinal antioxidant activities. The similar results were found in rat, Chinese mitten crab (Eriocheir inensis) and snout bream (Megalobrama amblycephala) (Miao et al, 2020;Shahid et al, 2018;Xu et al, 2019). whereas, the effects of dietary starch on the antioxidation activities are controversial.…”

Section: High-starch Diet Impaired Intestinal Physiological Barriersupporting

confidence: 64%

High-Starch Diet Consumption Impaired Intestinal Barriers of Juvenile Largemouth Bass (Micropterus Salmoides)

Zhao¹,

Ji²,

Liao³

et al. 2021

Preprint

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The intestinal barrier is primarily composed of physical, physiological and microbial barriers, and intestine microbe-immune interactions influenced by diet in fish still remain increasingly unexplored. The intestinal barriers of Largemouth bass (Micropterus salmoides) (4.1±0.2g) were explored after they were fed three different starch diets for eight weeks (low starch,7%; middle starch, 12%; high starch, 17%). The results showed that high starch diet led slight infiltration of inflammatory cells and moderate loss of mucous membrane layer in midgut. Meanwhile, high starch diet decreased the antioxidant enzymes (T-SOD) activities and genes (SOD1, SOD2, SOD3a, CAT, GPX) expression significantly (P < 0.05). The MDA content was significantly increased with increasing starch level (P < 0.05). High starch diet up-regulated the expression of pro-inflammatory genes (IL-8, IL-1β and TNFα) and apoptosis genes (bax, caspase3, caspase8 and caspase9), whereas down-regulated the expression of anti-apoptosis genes bcl-2 and tight junction proteins genes (occludin and claudin7). In addition, the high starch diet increased the relative abundance of Actinobacteria and Firmicutes, resulted in microbial dysbiosis. In conclusion, high dietary starch impaired intestinal barriers, and increased the risk of disease outbreaks.

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Section: High-starch Diet Impaired Intestinal Physiological Barriersupporting

confidence: 64%

High-Starch Diet Consumption Impaired Intestinal Barriers of Juvenile Largemouth Bass (Micropterus Salmoides)

Zhao¹,

Ji²,

Liao³

et al. 2021

Preprint

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“…Total RNA extraction and cDNA synthesis in liver (about 2 g) were performed by our previous studies [28,29,36]. Brie y, total RNA was extracted by Tri Pure Reagent (Aidlab, China).…”

Section: Analysis Of Western Blot (Wb) and Rt-pcrmentioning

confidence: 99%

Benfotiamine Ameliorates High Carbohydrate Diet-Induced Hepatic Oxidative Stress, Inflammation and Apoptosis in Megalobrama Amblycephala

Liu

Shi

et al. 2020

Preprint

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Background: The impairment of immunity induced by high-carbohydrate diet is closely associated with the development of glucose metabolic disorders. In the study of diabetes, benfotiamine can prevent β-cell dysfunction by inhibiting inflammation, thereby improving insulin resistance. However, information regarding the effects of this substance on aquatic animals is extremely scarce.Methods: A 12-week nutritional research was conducted to evaluate the influences of benfotiamine on the growth performance, oxidative stress, inflammation and apoptosis in Megalobrama amblycephala (45.25 ± 0.34 g) fed high-carbohydrate (HC) diets. Six experimental diets were formulated, containing a control diet (30% carbohydrate, C), a HC diet (43% carbohydrate), and the HC diet supplemented with four graded benfotiamine levels (0.7125 (HCB1), 1.425 (HCB2), 2.85 (HCB3), and 5.7 (HCB4) mg/kg).Results: HC diet intake remarkably decreased daily growth coefficient (DGC), growth rate per metabolic body weight (GRMBW), feed intake (FI), liver antioxidant enzymes activities, sirtuin-1 (SIRT1) protein expression as well as liver mRNA levels of SIRT1, nuclear factor erythroid 2-related factor 2 (Nrf2), catalase (CAT), manganese superoxide dismutase (Mn-SOD), interleukin10 (IL10) than those of the control group, but the opposite was true for plasma activities of alanine transaminase (AST) and aspartate aminotransferase (ALT), and contents of interleukin 1β (IL1β) and interleukin 6 (IL6), liver contents of malondialdehyde (MDA), and mRNA levels of kelch-like ECH associating protein 1 (Keap1), nuclear factor kappa B (NF-κB), tumour necrosis factor α (TNF α), IL1β, IL6, Bax, Caspase 3, Caspase 9 and P53. As with benfotiamine supplementation, HCB2 diet remarkably increased DGC, GRMBW, liver antioxidant enzymes activities, SIRT1 protein expression as well as liver mRNA levels of SIRT1, Nrf2, CAT, Mn-SOD, IL10 and Bcl2, while the opposite was true for plasma activities of AST and ALT, and contents of IL1β and IL6, liver MDA contents as well as mRNA levels of Keap1, NF-κB, TNF α, IL1β, IL6, Bax, Caspase 3, Caspase 9 and P53.Conclusion: Benfotiamine at 1.425 mg/kg can improve the growth performance and alleviate the oxidative stress, inflammation and apoptosis of M. amblycephala fed HC diets through the activation of the SIRT1 pathway.

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“…According to previous studies, thiamine activates AMPK, thereby enhancing mitochondrial function by modulating the gene networks controlling mitochondrial biogenesis (51,66) . In addition, the activated AMPK can also enhance anti-oxidative enzyme activities by activating the NF-E2-related factor 2 mediated antioxidant pathway, thus facilitating the clearance of ROS (43) . This could further promote the mitochondrial biogenesis and function.…”

Section: Discussionmentioning

confidence: 99%

“…The activities of cytochrome c oxidase and F1F0-ATP synthase (complex IV) were analyzed according to Kirby et al (2007) (42) . Total RNA extraction and cDNA synthesis in the frozen intestine (about 2 g) were performed as described in our previous study (43,44) . The transcriptional levels were determined for the following genes, including AMP-activated protein kinase α 1 and 2 (AMPKα 1 and 2), peroxisome proliferator activated receptor-γ coactivator-1α and 1β (PGC-1α and 1β), mitochondrial transcription factor A (TFAM), mitofusin-1 (mfn-1), optic atrophy-1 (opa-1), dynamin-related protein-1 (drp-1), fission-1 (fis-1), mitochondrial fission factor (mff); NADH dehydrogenase-1 (ND-1), cytochrome B (CYTB), cytochrome c oxidase-1 and 2 (COX-1 and 2), sodium/glucose cotransporter-1 (SGLT-1) and glucose transporter-2 (GLUT-2).…”

Section: Analysis Of Intestinal Ros Levels Mitochondrial Membrane Pomentioning

confidence: 99%

Interactions between dietary carbohydrate and thiamine: implications on the growth performance and intestinal mitochondrial biogenesis and function of Megalobrama amblycephala

You

Brown

et al. 2021

Br J Nutr

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A 12-week experiment was conducted to evaluate the influences of thiamine on growth performance and intestinal mitochondrial biogenesis and function of M. amblycephala fed a high-carbohydrate (HC) diet. Fish (24.73 ± 0.45 g) were randomly assigned one of four diets; two carbohydrate levels (30 and 45%) and two thiamine levels (0 and 1.5 mg kg-1). HC diets significantly decreased DGC, GRMBW, FIMBW, intestinal activities of amylase, lipase, Na+, K+-ATPase, CK, complex I, III and IV, intestinal microvilli length, number of mitochondrial per field, ΔΨm, the P-AMPK/T-AMPK ratio, PGC-1β protein expression as well as the transcriptions of AMPKα1, AMPKα2, PGC-1β, TFAM, Opa-1, ND-1 and COX-1 and 2, while the opposite was true for ATP, AMP and ROS, and the transcriptions of Drp-1, Fis-1 and Mff. Dietary thiamine concentrations significantly increased DGC, GRMBW, intestinal activities of amylase, Na+, K+-ATPase, CK, complex I and IV, intestinal microvilli length, number of mitochondrial per field, ΔΨm, the P-AMPK/T-AMPK ratio, PGC-1β protein expression as well as the transcriptions of AMPKα1, AMPKα2, PGC-1β, Opa-1, ND-1, COX-1 and 2, SGLT-1 and GLUT-2. Furthermore, a significant interaction between dietary carbohydrate and thiamine was observed in DGC, GRMBW, intestinal activities of amylase, CK, complex I and IV, ΔΨm, the AMP/ATP ratio, the P-AMPK/T-AMPK ratio, PGC-1β protein expression as well as the transcriptions of AMPKα1, AMPKα2, PGC-1β, Opa-1, COX-1 and 2, SGLT-1 and GLUT-2. Overall, thiamine supplementation improved growth performance, and intestinal mitochondrial biogenesis and function of M. amblycephala fed HC diets.

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Feeding restriction alleviates high carbohydrate diet-induced oxidative stress and inflammation of Megalobrama amblycephala by activating the AMPK-SIRT1 pathway

Cited by 42 publications

References 85 publications

High-Starch Diet Consumption Impaired Intestinal Barriers of Juvenile Largemouth Bass (Micropterus Salmoides)

High-Starch Diet Consumption Impaired Intestinal Barriers of Juvenile Largemouth Bass (Micropterus Salmoides)

Benfotiamine Ameliorates High Carbohydrate Diet-Induced Hepatic Oxidative Stress, Inflammation and Apoptosis in Megalobrama Amblycephala

Interactions between dietary carbohydrate and thiamine: implications on the growth performance and intestinal mitochondrial biogenesis and function of Megalobrama amblycephala

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