Feline infectious peritonitis virus antibody test using enzyme-linked immunosorbent assay.

Ishida, Takuo; Washizu, Tsukimi; Fukuoka, Jun; Toriyabe, Kazushige; Uchino, Tomiya; Motoyoshi, Shigekatsu

doi:10.1292/jvms1939.49.145

Cited by 7 publications

(4 citation statements)

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“…Several results concerning the state of FIP incidence have been reported overseas [6,15,16,18,29]. In Japan, there have been several reports on the prevalence of FCoV infection [11,13,20,24], but, to our knowledge, no studies on FIP prevalence have been reported. Thus, in this study, to investigate the state of FIP incidence in Japan, we examined FCoV RNA in ascites of domestic cats suspected of having FIP using RT-PCR and investigated the genotype of detected FCoV RNA.…”

mentioning

confidence: 94%

Detection of Ascitic Feline Coronavirus RNA from Cats with Clinically Suspected Feline Infectious Peritonitis

Soma

Wada

Taharaguchi

et al. 2013

The Journal of Veterinary Medical Science

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Ascitic feline coronavirus (FCoV) RNA was examined in 854 cats with suspected feline infectious peritonitis (FIP) by RT-PCR. The positivity was significantly higher in purebreds (62.2%) than in crossbreds (34.8%) (P<0.0001). Among purebreds, the positivities in the Norwegian forest cat (92.3%) and Scottish fold (77.6%) were significantly higher than the average of purebreds (P=0.0274 and 0.0251, respectively). The positivity was significantly higher in males (51.5%) than in females (35.7%) (P<0.0001), whereas no gender difference has generally been noted in FCoV antibody prevalence, indicating that FIP more frequently develops in males among FCoV-infected cats. Genotyping was performed for 377 gene-positive specimens. Type I (83.3%) was far more predominantly detected than type II (10.6%) (P<0.0001), similar to previous serological and genetic surveys.

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mentioning

confidence: 94%

Detection of Ascitic Feline Coronavirus RNA from Cats with Clinically Suspected Feline Infectious Peritonitis

Soma

Wada

Taharaguchi

et al. 2013

The Journal of Veterinary Medical Science

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“…Serological surveys of FCoV infection have involved detection of antibody by indirect fluorescent antibody assay (IFA) or enzyme-linked immunosorbent assay (ELISA) [12][13][14][15]. A plaque-reduction neutralization test (PRNT) was developed to serologically distinguish FCoV type I and II infections in cats [16].…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Korean cats with natural feline coronavirus infections

Jeoung

Jeong

et al. 2011

Virol J

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BackgroundFeline coronavirus is comprised of two pathogenic biotypes consisting of feline infectious peritonitis virus (FIPV) and feline enteric coronavirus (FECV), which are both divided into two serotypes. To examine the prevalence of Korean cats infected with feline coronavirus (FCoV) type I and II, fecal samples were obtained from 212 cats (107 pet and 105 feral) in 2009.ResultsFourteen cats were FCoV-positive, including infections with type I FCoV (n = 8), type II FCoV (n = 4), and types I and II co-infection (n = 2). Low seroprevalences (13.7%, 29/212) of FCoV were identified in chronically ill cats (19.3%, 16/83) and healthy cats (10.1%, 13/129).ConclusionsAlthough the prevalence of FCoV infection was not high in comparison to other countries, there was a higher prevalence of type I FCoV in Korean felines. The prevalence of FCoV antigen and antibody in Korean cats are expected to gradually increase due to the rising numbers of stray and companion cats.

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“…Furthermore, type II FCoV shows close antigenic and genetic relationship to CCoV and TGEV, suggesting that FCoV type II arose from double recombination between FCoV type I and CCoV (Herrewegh et al, 1998) FCoV type II strains are more frequently utilized than type I strains for in vitro experiments because of higher efficient growth. For serological survey of FCoV infection, antibody has been detected by indirect fluorescent antibody assay (IFA) or enzyme-linked immunosorbent assay (ELISA) using FCoV (Pedersen, 1976;Scott, 1979;Horzinek and Osterhaus, 1979;Ishida et al, 1987). Alternatively CCoV or TGEV are also used as a target antigen because of serological close relations to FCoV.…”

Section: Introductionmentioning

confidence: 99%

Differentiation of feline coronavirus type I and II infections by virus neutralization test

Shiba

Maeda

Kato

et al. 2007

Veterinary Microbiology

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Feline coronavirus (FCoV) is divided into two types I and II, based on their growth in vitro and antigenicity. In this study, virus neutralization (VN) test was applied for type differentiation of FCoV infections. Sera of cats which were clinically and serologically diagnosed as feline infectious peritonitis (FIP) possessed significantly higher VN titers to type I FCoV, and sera from cats experimentally infected with FIPV type II had high VN titers to type II but not type I viruses. A total of 79 cat sera collected in the years between 2004 and 2005 were examined to evaluate seroprevalence by the VN test, showing the following results: (1) 50 cats (63.3%) were sero-positive to FCoV; (2) of the 50 FCoV positive cat serum samples, 49 (98%) showed significantly higher titers to type I virus and only one (2%) for type II virus. These results indicate that the VN test described here can be used for serological differentiation of FCoV infections of cats, and that FCoV type I is a dominant type in recent years of Japan.

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Feline infectious peritonitis virus antibody test using enzyme-linked immunosorbent assay.

Cited by 7 publications

References 0 publications

Detection of Ascitic Feline Coronavirus RNA from Cats with Clinically Suspected Feline Infectious Peritonitis

Detection of Ascitic Feline Coronavirus RNA from Cats with Clinically Suspected Feline Infectious Peritonitis

Prevalence of Korean cats with natural feline coronavirus infections

Differentiation of feline coronavirus type I and II infections by virus neutralization test

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