Fermentation Studies with
            <i>Streptomyces griseus</i>

Shirato, Shiroh; Motoyama, Hiroshi

doi:10.1128/am.14.5.706-710.1966

Appl Microbiol

1966

DOI: 10.1128/am.14.5.706-710.1966

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Fermentation Studies with Streptomyces griseus

Shiroh Shirato

Hiroshi Motoyama

Abstract: Synthetic media for streptomycin fermentation were studied to determine which media gave highest yields of streptomycin. The effect of salts on streptomycin production by Streptomyces griseus was examined, and a suitable combination of salts was established in a glucose-casein medium. This medium yielded 3,000 μg/ml of the antibiotic with an inoculum of 1.6%. Substitution of amino acids for casein was examined. Of 17 amino acids tested, best results were obtaind with sodium aspartate. S… Show more

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Cited by 7 publications

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Development of a defined medium fermentation process for physostigmine production by Streptomyces griseofuscus

Zhang

Marcin

et al. 1996

Appl Microbiol Biotechnol

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Physostigmine is a plant alkaloid of great interest as a therapeutic candidate for the treatment of Alzheimer's disease. Fortunately, this compound is also produced by Streptomyces griseofuscus NRRL 5324 during submerged cultivation. A fermentation process that used chemically defined medium was therefore developed for its production. By means of statistical experimentation, the physostigmine titer was quickly increased from 20 mg/l to 520 mg/l with a culture growth of 19 gl dry cell weight on the shake-flask scale. Further medium optimization resulted in a yield of 790 mg/l in a 23-1 bioreactor using a batch process. A titer of 880 mg/l was attained during scale-up in a 800-1 fermentor by employing a nutrient-feeding strategy. This production represents a 44-fold increase over the yield from the initial process in shake-flasks. The defined-medium fermentation broth was very amenable to downstream processing.

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Development of a defined medium fermentation process for physostigmine production by Streptomyces griseofuscus

Zhang

Marcin

et al. 1996

Appl Microbiol Biotechnol

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Biosynthesis of streptomycin dTDP-Dihydrostreptose synthase from Streptomyces griseus and dTDP-4-keto-l-rhamnose 3,5-epimerase from S. griseus and Escherichia coli Y10

Wahl

Grisebach

1979

Biochimica et Biophysica Acta (BBA) - Enzymology

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NADPH‐Dependent Formation of Thymidine diphosphodihydrostreptose from Thymidine diphospho‐d‐glucose in a Cell‐Free System from Streptomyces griseus and Its Correlation with Streptomycin Biosynthesis

Ortmann

Matern

Grisebach

et al. 1974

European Journal of Biochemistry

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When thymidine diphospho-~-[~-~~C]glucosewas incubated with a cell-free extract from a streptomycin-producing strain of Xtreptomyces griseus in the presence of NADPH, radioactive dTDP-dihydrosteptose was formed in addition to radioactive dTDP-4-keto-6-deoxyglucose and dTDP-rhamnose. Dihydrostreptose was identified by chromatographic, gas chromatographic and electrophoretic comparison with synthetic dihydrostreptose characterized by its nuclear magnetic resonance spectrum.2. dTDP-dihydrostreptose is very labile and decomposes to dihydrostreptose monophosphate and further t o free dihydrostreptose.3. dTDP-dihydrostreptose and dTDP-rhamnose were also obtained when dTDP-4-keto 6-deoxyglucose was the substrate of the reaction. Dihydrostreptose synthesis could be inhibited completely by 1 mM p-chloromercuribenzoate, whereas rhamnose formation was not inhibited.4. I n the course of fermentation the activities of arginine : x-itmidinotransferase, an enzyme involved in streptomycin biosynthesis, and of the enzymatic activity for dTDP-dihydrostreptose formation ran parallel and reached a maximum before onset of streptomycin formation. 5. A good correlation between the activity of "dTDP-dihydrostreptose synthase", arginine : x-amidinotransferase activity and streptomycin production was found in several strains of S . griseus. No dihydrostreptose formation could be detected in a hydroxystreptomycin-producing strain of S . griseocarneus. 6 . Streptomycin but not dihydrostreptomycin could be detected in the fermentation medium of the S. griseus strain used for preparation of the cell-free extract. The role of dTDP-dihydrostreptose in streptomycin biosynthesis is at present unknown.Through studies in vivo with labeled precursors it was shown that L-streptose (5-deoxy-3-C-formyl-L-lyxo-aldopentofuranose) in streptomycin is derived from D-glucose by a rearrangement of the hexose chain in which C-3 of glucose becomes the 3-C-formyl branch [1,2]. On the basis of the observation that thymidine diphospho -mannose (dTDP -mannose) and dTDP-rhamnose occur in Streptomyces griseus [3] and that cell-free preparations from this organism can convert both dTDP-glucose and dTDP-mannose into dTDP-rhamnose [4], it was suggested that the biosynthesis of streptose and that of rhamnose are related. So far, however, neither nucleotides containing streptose nor a cell-free system for streptose formation have been found. I n this communication we report on an enzyme preparation from S. griseus catalyzing the NADPH-dependent formation of a Eur. J. Biochem. 43 (1974)

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Fermentation Studies with Streptomyces griseus

Cited by 7 publications

References 7 publications

Development of a defined medium fermentation process for physostigmine production by Streptomyces griseofuscus

Development of a defined medium fermentation process for physostigmine production by Streptomyces griseofuscus

Biosynthesis of streptomycin dTDP-Dihydrostreptose synthase from Streptomyces griseus and dTDP-4-keto-l-rhamnose 3,5-epimerase from S. griseus and Escherichia coli Y10

NADPH‐Dependent Formation of Thymidine diphosphodihydrostreptose from Thymidine diphospho‐d‐glucose in a Cell‐Free System from Streptomyces griseus and Its Correlation with Streptomycin Biosynthesis

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