Neoseiulus californicus has been increasing in number and geographical distribution since the 1990s in Japan, and is considered to be a new prospective predator species against Panonychus citri on citrus. We investigated the occurrence of N. californicus in citrus orchards with different agrochemical spraying programs in Kyushu district, south-western Japan. The occurrence of N. californicus greatly differed among orchards. In conventional control orchards and agrochemical-reduced orchards, N. californicus females were observed in 50-60% of orchards. On the other hand, N. californicus females were rare in agrochemical-free orchards. N. californicus females were observed in all prefectures in the Kyushu district, but the occurrence of N. californicus was different among the § 果樹研究所業績番号：1465

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SHIRATO, SHIROH (Kaken Chemical Co., Ltd., Tokyo, Japan), AND HIROSHI MOTOYAMA. Fermentation studies with Streptomyces griseus II. Synthetic media for the production of streptomycin. Appl. Microbiol. 14:706-710. 1966.-Synthetic media for streptomycin fermentation were studied to determine which media gave highest yields of streptomycin. The effect of salts on streptomycin production by Streptomyces griseus was examined, and a suitable combination of salts was estab

Synthetic media for streptomycin fermentation were studied to determine which media gave highest yields of streptomycin. The effect of salts on streptomycin production by Streptomyces griseus was examined, and a suitable combination of salts was established in a glucose-casein medium. This medium yielded 3,000 μg/ml of the antibiotic with an inoculum of 1.6%. Substitution of amino acids for casein was examined. Of 17 amino acids tested, best results were obtaind with sodium aspartate. Substitution of ammonium salts was tried, and an excellent streptomycin yield was obtained with a medium containing ammonium citrate.

Kaken Chemical Co., Ltd., Bunkyo-ku, Tokyo, Japan), AND CHIZUKo NAGATSU. Fermentation studies with Streptomyces griseus. I. Carbohydrate sources for the production of protease and streptomycin. Appl. Microbiol. 13:669-672. 1965.-The effects of four carbon sources and inorganic phosphate on the production of streptomycin and protease by a strain of Streptomyces griseus were studied. Protease production was increased in fermentations with comparatively rapid consumption of carbohydrate, and streptomycin was produced under conditions of moderately slow consumption. Starch was consumed more rapidly than glucose, and, in fermentations with starch as a carbon source, good yields of protease were associated with poor yields of streptomycin. The effect of the concentration of inorganic phosphate varied with the sugar source; the rate of consumption of glucose or fructose increased with the addition of inorganic phosphate, and the utilization of starch or maltose was not affected.