Fertility of ram semen frozen in Bioexcell® and used for cervical artificial insemination

Gil, J.

doi:10.1016/s0093-691x(02)01178-0

Cited by 96 publications

(69 citation statements)

References 26 publications

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“…The use of Bioxcell ® in this study was the best extender for fresh deer semen, compared to the other two, which coincides with Gil et al (2003), who reported that Bioxcell ® is an alternative for the conservation of ram semen. Similarly, Sariözkan et al (2010), demonstrated that the use of Bioxcell ® with or without centrifugation and sperm washing, facilitates the conservation of fresh semen in goats.…”

Section: Effect Of Diluentssupporting

confidence: 90%

Commercial extenders and freezing curves for the preservation of sperm cells of white-tailed deer (Odocoileus virginianus)

Clemente-Sánchez

Cessa-Reyes

Cortez‐Romero

et al. 2014

Journal of Applied Animal Research

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During the reproductive season of the white-tailed deer, 36 diluted semen samples from deer were evaluated before freezing, and at the time of thawing. The samples were obtained by electro ejaculation from three sedated deer with a mixture of Zoletil ® and xylazine hydrochloride. Samples were diluted with three commercial extenders Biladyl ® , Trilady ® and Bioxcell ® and frozen by applying two freezing curves, the one used for sheep and goat, and the recommended for deer semen. The response variable was the Semen Quality Index for Deer (SQID%) obtained from the estimation of the percentage of gross motility, progressive motility, alive sperms, normal sperms and sperm concentration. The results of the statistical analysis showed differences (p = 0.0301) between the extenders before freezing, resulting Bioxcell ® with greater SQID (60.77%), while for Triladyl ® and Biladil ® rates were 48.39% and 33.01%, respectively. As for the evaluated freezing curves, no differences were found (p = 0.5502) between the recommended curve for deer semen and the curve used for sheep and goat semen. In the present study, the use of the SQID as response variable turned out to be useful in the assessment of diluents and freezing curves.

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Section: Effect Of Diluentssupporting

confidence: 90%

Commercial extenders and freezing curves for the preservation of sperm cells of white-tailed deer (Odocoileus virginianus)

Clemente-Sánchez

Cessa-Reyes

Cortez‐Romero

et al. 2014

Journal of Applied Animal Research

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“…É conhecido o fato de ocorrerem variações individuais entre animais ou mesmo entre ejaculados de um mesmo animal 3,33,34 . Essa variabilidade individual pode ocorrer na composição proteica do plasma seminal entre animais 35,36 .…”

Section: Introductionunclassified

Criopreservação do sêmen ovino em meio diluente à base de água de coco em pó (ACP-102c)

Cavalcante

Brasil²,

Salgueiro

et al. 2014

Ciênc. anim. bras.

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RESUMOO objetivo deste trabalho foi avaliar o diluente ACP-102c na criopreservação do sêmen ovino em comparação com o diluidor tris-glicose-gema (TRIS) e o sêmen fresco. Foram coletados 48 ejaculados de quatro ovinos, sendo tomadas duas alíquotas por ejaculado para diluição e criopreservação em ACP-102c ou TRIS e uma terceira alíquota utilizada para análise do sêmen fresco. O sêmen fresco e o criopreservado em ambos os diluidores foram avaliados para viabilidade, integridade de membrana plasmática e acrossomal, teste hiposmótico, fragmentação do DNA e de motilidade espermática. Após descongelamento, ambos os diluidores não diferiram para viabilidade espermática, integridade de membrana plasmática e acrossomal, fragmentação de DNA e nas variáveis quantitativas e qualitativas de velocidade espermática, mas diferiram no teste hiposmótico, motilidade total e progressiva e amplitude lateral da cabeça, bem como em todas as variáveis de motilidade avaliadas, exceto linearidade e progressividade, após duas horas de incubação à 37 o C. Houve variabilidade entre reprodutores na motilidade total e progressiva do sêmen criopreservado em ACP-102c após descongelamento. O diluidor ACP-102c conferiu menor proteção aos espermatozoides ovinos contra danos do congelamento quando comparado ao TRIS, mas o aprimoramento de sua formulação e protocolos mais adequados de congelação poderão torná-lo uma alternativa na congelação do sêmen ovino.PALAVRAS-CHAVE: ACP-102c; criopreservação; sêmen ovino. CRYOPRESERVATION OF RAM SEMEN IN POWDERED COCONUT WATER (ACP-102c) BASED EXTENDER ABSTRACTThe aim of this study was to evaluate the ACP-102c extender in the cryopreservation of ram semen compared to tris-glucose-egg yolk (TRIS) extender and fresh semen. Forty-eight ejaculates were collected from four rams and two aliquots per ejaculate were taken for dilution and cryopreservation in ACP-102c or TRIS and a third aliquot used for the fresh semen analysis. Either the fresh semen and cryopreserved in both extenders were evaluated for viability, integrity of plasma and acrosomal membrane, hypoosmotic swelling test, DNA fragmentation and sperm motility. The extenders did not differ for sperm viability, acrosome and plasma membrane integrity, DNA fragmentation and quantitative and qualitative parameters of sperm velocity after thawing, but differed in hypoosmotic swelling test, total and progressive motility and lateral extent of the head as well as in all motility parameters evaluated (except linearity and straightness) after two hours of incubation at 37 o C. There was variability among rams in total and progressive motility of semen cryopreserved in ACP-102c after thawing. The

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“…El más empleado ha sido la yema de huevo por su contenido de lipoproteínas de baja densidad (LDL), la cual es la responsable del efecto crioprotector (5,6). Sin embargo, el uso de yema de huevo puede incrementar el riesgo de contaminación microbiana y reducir la capacidad fecundante de los spz.…”

Section: Introduccionunclassified

Las proteínas del plasma seminal incrementan la viabilidad espermática post-descongelación del semen de toros Sanmartinero

et al. 2013

Rev MVZ Córdoba

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RESUMENObjetivo. El objetivo de este trabajo fue evaluar el efecto de la adición de proteínas del plasma seminal sobre el porcentaje de espermatozoides bovinos viables post-descongelación. Materiales y métodos. Los espermatozoides se congelaron usando dos medios (citrato-fructosa-yema y Bioxcell ® ) y la obtención de proteínas de plasma seminal de bajo peso molecular se realizó por medio de cromatografía líquida de baja presión. Las proteínas de interés eluyeron en las fracciones 21-25 y se sometieron a electroforésis en una y dos dimensiones. Los espermatozoides se incubaron a 37°C durante una hora, con 0.5, 1.0, 1.5 y 2.0 mg de la fracción 21-25. Se incluyeron dos tratamientos adicionales: uno con proteínas totales del plasma seminal y otro sin proteína. Resultados. La electroforésis bidimensional de las fracciones confirmó la presencia de siete puntos de proteína de bajo peso molecular (14-16 kDa y punto Isoeléctrico de 5.0 -5.5). La adición de estas proteínas aumentó 20% (p<0.05), el porcentaje de espermatozoides viables post-descongelación en muestras congeladas en medio citrato-fructosayema (con dosis de 1 ó 1.5 mg de proteína/10 6 espermatozoides), y 25% (p<0.05) en muestras congeladas en medio Bioxcell ® (con dosis de 0.5 mg de proteína/10 6 espermatozoides). Conclusiones. Los resultados de esta investigación sugieren el posible uso de proteínas de bajo peso molecular del plasma seminal, para disminuir el efecto deletéreo de la criopreservación en los espermatozoides.Palabras clave: Capacitación espermática, criopreservación, espermatozoide, plasma seminal, proteínas de choque térmico (Fuente: DeCS).

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Fertility of ram semen frozen in Bioexcell® and used for cervical artificial insemination

Cited by 96 publications

References 26 publications

Commercial extenders and freezing curves for the preservation of sperm cells of white-tailed deer (Odocoileus virginianus)

Commercial extenders and freezing curves for the preservation of sperm cells of white-tailed deer (Odocoileus virginianus)

Criopreservação do sêmen ovino em meio diluente à base de água de coco em pó (ACP-102c)

Las proteínas del plasma seminal incrementan la viabilidad espermática post-descongelación del semen de toros Sanmartinero

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