Fertilization: a sperm’s journey to and interaction with the oocyte

Ikawa, Masahito; Inoue, Naokazu; Benham, Adam M.; Okabe, Masaru

doi:10.1172/jci41585

Cited by 278 publications

(236 citation statements)

References 134 publications

Supporting

Mentioning

232

Contrasting

Unclassified

Order By: Relevance

“…S tructural cell migration is a central mechanism involved in a range of physiological conditions, such as wound healing (1), neurogenesis (2), and embryogenesis (3). This very fundamental mechanism may have detrimental consequences for tissue structure and function when migration leads to an excessive accumulation of cells.…”

mentioning

confidence: 99%

CXCL1 Inhibits Airway Smooth Muscle Cell Migration through the Decoy Receptor Duffy Antigen Receptor for Chemokines

Al-Alwan

Chang

Rousseau

et al. 2014

The Journal of Immunology

View full text Add to dashboard Cite

Airway smooth muscle cell (ASMC) migration is an important mechanism postulated to play a role in airway remodeling in asthma. CXCL1 chemokine has been linked to tissue growth and metastasis. In this study, we present a detailed examination of the inhibitory effect of CXCL1 on human primary ASMC migration and the role of the decoy receptor, Duffy AgR for chemokines (DARC), in this inhibition. Western blots and pathway inhibitors showed that this phenomenon was mediated by activation of the ERK-1/2 MAPK pathway, but not p38 MAPK or PI3K, suggesting a biased selection in the signaling mechanism. Despite being known as a nonsignaling receptor, small interference RNA knockdown of DARC showed that ERK-1/2 MAPK activation was significantly dependent on DARC functionality, which, in turn, was dependent on the presence of heat shock protein 90 subunit α. Interestingly, DARC- or heat shock protein 90 subunit α–deficient ASMCs responded to CXCL1 stimulation by enhancing p38 MAPK activation and ASMC migration through the CXCR2 receptor. In conclusion, we demonstrated DARC’s ability to facilitate CXCL1 inhibition of ASMC migration through modulation of the ERK-1/2 MAPK–signaling pathway.

show abstract

mentioning

confidence: 99%

CXCL1 Inhibits Airway Smooth Muscle Cell Migration through the Decoy Receptor Duffy Antigen Receptor for Chemokines

Al-Alwan

Chang

Rousseau

et al. 2014

The Journal of Immunology

View full text Add to dashboard Cite

show abstract

“…In most of the mammals studied, only spermatozoa with an intact acrosome bind to the ZP, in a speciesspecifi c manner. The fertilizing spermatozoan interaction with the zona glycoprotein that serves as sperm receptor (ZP3/ZPC in mouse/human and ZPB/ZPC complex in pig) triggers the AE via signal transduction events in the sperm acrosome, such as the opening of T-type, low voltage-activated Ca 2+ channels, causing an infl ux of Ca 2+ and the activation of heterotrimeric G proteins (Pasten et al 2005 ;Ikawa et al 2010 ). An increase in intracellular pH, resulting in sustained Ca 2+ infl ux, which drives AE, causes the release or exposure of acrosomal enzymes and, when combined with vigorous sperm motility, allows the spermatozoon to penetrate the ZP and fuse with the oocyte plasma membrane, the oolemma (Pasten et al 2005 ;Ikawa et al 2010 ).…”

Section: Proteasome Localization and Activity In Mammalian Spermatozoamentioning

confidence: 99%

Sperm Proteasome as a Putative Egg Coat Lysin in Mammals

Miles

Šutovský

2014

Sexual Reproduction in Animals and Plants

View full text Add to dashboard Cite

During animal and human fertilization, the fertilizing spermatozoon creates and passes through a fertilization slit in the vitelline coat (VC) of the oocyte. It has been hypothesized that the penetration of the mammalian VC, the zona pellucida (ZP), is aided by a proteolytic enzyme capable of locally degrading ZP proteins. This putative "zona lysin" is predicted to reside within the sperm head acrosome and be released or exposed by ZP-induced acrosomal exocytosis. Evidence has been accumulating in favor of the 26S proteasome, the ubiquitin-dependent multi-subunit protease acting as the putative vitelline coat/zona lysin in humans and animals. To confi rm this hypothesis, three criteria must be met: (1) the sperm receptor on the ZP must be ubiquitinated, (2) proteasomes must be present, exposed, and enzymatically active in the sperm acrosome, and (3) sperm proteasomes must be able to degrade the sperm receptor on the egg coat/ZP during fertilization. This review discusses recent data from a number of mammalian and nonmammalian models addressing these predictions. These data shed light on the mechanisms controlling sperm interactions with VC and on the evolutionary conservation of the proteasome-assisted fertilization mechanisms.

show abstract

“…Subsequently, two pronuclei are formed, DNA replication taking place in each of them, and the first mitotic division is initiated when the chromosomes of the oocyte and sperm form a common metaphase plate. Differences among species can pertain to asynchrony in pronuclei formation and the moment of replication initiation (Gadella, 2010;Ikawa et al, 2010;Visconti and Florman, 2010;Tulsiani et al, 2011).…”

Section: In Vitro Fertilization Of Matured Oocytesmentioning

confidence: 99%

Obtaining farm animal embryos <i>in vitro</i>

Duszewska¹,

Rapala²,

Trzeciak³

et al. 2012

J. Anim. Feed Sci.

View full text Add to dashboard Cite

This paper reviews the basic knowledge about obtaining farm animal embryos in vitro with special focus on differences among species and application of this procedure in the future. In vitro production of farm animal embryos consists of in vitro maturation (IVM) of oocytes, in vitro fertilization (IVF) of matured oocytes, and in vitro culture (IVC) of embryos. Oocytes can be collected from live animals (by laparotomy, laparoscopy, Ovum Pick Up) or from slaughtered ones (by puncture, sectioning). Usually immature oocytes are isolated, and during IVM they reach maturity. Matured oocytes are cultured with sperm (IVF), leading to the formation of zygotes. In the case of fertilization problems (horse, pig), intracytoplasmic sperm injection is used. The zygotes are usually cultured (IVC) to the morula and blastocyst stages. These embryos can be transferred to recipients or frozen/vitrified. Offspring have been obtained after transfer of cattle, sheep, goat, pig and horse embryos. This procedure can be used in animal breeding, biotechnology, medicine, and basic research.

show abstract

Fertilization: a sperm’s journey to and interaction with the oocyte

Cited by 278 publications

References 134 publications

CXCL1 Inhibits Airway Smooth Muscle Cell Migration through the Decoy Receptor Duffy Antigen Receptor for Chemokines

CXCL1 Inhibits Airway Smooth Muscle Cell Migration through the Decoy Receptor Duffy Antigen Receptor for Chemokines

Sperm Proteasome as a Putative Egg Coat Lysin in Mammals

Obtaining farm animal embryos <i>in vitro</i>

Contact Info

Product

Resources

About