Fertilization of C57BL/6 mouse sperm collected from cauda epididymides after preservation or transportation at 4°C using laser-microdissected oocytes

Kaneko, Takehito; Fukumoto, Kiyoko; Haruguchi, Yukie; Kondo, Tomoko; Machida, Hiromi; Koga, Mika; Nakagawa, Yoshiko; Tsuchiyama, Shuuji; Saiki, Kiyora; Noshiba, Shiho; Nakagata, Naomi

doi:10.1016/j.cryobiol.2009.04.006

Cited by 25 publications

(34 citation statements)

References 20 publications

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“…Laser-zona drilling (LZD) has been recognized for being one of the most promising methods to assist IVF in humans and mice (El-Danasouri et al 1993, Laufer et al 1993, Antinori et al 1994, Liow et al 1996, Kaneko et al 2006, 2009). LZD has also been used to biopsy the polar body and blastomere for genetic diagnosis of oocytes and embryos (Montag et al 2004, Harper et al 2010), to assist embryo hatching (Hammadeh et al 2011) and to facilitate the injection of embryonic stem cells into morulae or blastocysts to produce GM mice (Pluück & Klasen 2009).…”

Section: Introductionmentioning

confidence: 99%

“…Although lasers of varying wavelengths (0.248, 0.308, 0.337, 1.48, 2.94 µm, etc.) have been tested mice and humans the infrared (IR) laser at wavelength 1.45–1.48 µm is preferred (Antinori et al 1994, Rink et al 1994, Germond et al 1995, Schoöpper et al 1999, Peters et al 2006, Kaneko et al 2009). IR lasers at these latter wavelengths allow noncontact, microscope objective-delivered.…”

Section: Introductionmentioning

confidence: 99%

“…LZD has been shown to significantly increase fertilization rates in mice (El-Danasouri et al 1993, Enginsu et al 1995, Germond et al 1996, Liow et al 1996, Anzai et al 2006, Kaneko et al 2006, 2009, Boersma et al 2007) and humans (Obruca et al 1994) with low quality sperm. Mouse embryos derived by LZD-assisted IVF have been shown to develop to the blastocyst stage in vitro at a rate similar to that of embryos derived by regular IVF (El-Danasouri et al 1993, Enginsu et al 1995, Kaneko et al 2009).…”

Section: Introductionmentioning

confidence: 99%

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Safety, efficacy and efficiency of laser-assisted IVF in subfertile mutant mouse strains

Kinchen

Vallelunga

et al. 2013

Reproduction

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In the present report we studied the safety, efficacy and efficiency of using an infrared laser to facilitate IVF by assessing fertilization, development and birth rates after laser-zona drilling (LZD) in 30 subfertile genetically modified (GM) mouse lines. We determined that LZD increased the fertilization rate four to ten times that of regular IVF, thus facilitating the derivation of 26 of 30 (86.7%) GM mouse lines. Cryopreserved two-cell stage embryos derived by LZD-assisted IVF were recovered and developed to blastocysts in vitro at the same rate as frozen–thawed embryos derived by regular IVF. Surprisingly after surgical transfer to pseudopregnant recipients the birth rate of embryos derived by LZD-assisted IVF was significantly lower than that of embryos derived by regular IVF. However this result could be completely mitigated by the addition of 0.25 M sucrose to the culture medium during LZD which caused the oocyte to shrink in volume relative to the perivitelline space. By increasing the distance from the laser target site on the zona pellucida, we hypothesize that the hyperosmotic effect of sucrose reduced the potential for laser-induced cytotoxic thermal damage to the underlying oocytes. With appropriate preparation and cautious application, our results indicate that LZD-assisted IVF is a safe, efficacious and efficient assisted reproductive technology for deriving mutant mouse lines with male factor infertility and subfertility caused by sperm–zona penetration defects.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Safety, efficacy and efficiency of laser-assisted IVF in subfertile mutant mouse strains

Kinchen

Vallelunga

et al. 2013

Reproduction

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“…Numerous studies have reported that sperm storage (without loss of viability) is only possible for short periods (e.g., 48 hours) at ambient or refrigeration temperatures [9; 10; 12; 15; 24]. These reports document the difficulties associated with maintaining normal mouse sperm motility and viability in vitro for more than 48 hours.…”

Section: Discussionmentioning

confidence: 99%

“…The cold transport of fresh mouse embryos or cauda epididymides is often done among research facilities [11; 12; 13; 15; 32; 33; 36]. It is typically a low-cost and low-risk alternative to live animal transport.…”

Section: Introductionmentioning

confidence: 99%

Investigations of motility and fertilization potential in thawed cryopreserved mouse sperm from cold-stored epididymides

et al. 2014

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Cold transport of epididymides from genetically modified mice is an efficient alternative to the shipment of live animals between research facilities. Mouse sperm from epididymides cold-stored for short periods can maintain viability. We previously reported that cold storage of mouse epididymides in Lifor® perfusion medium prolonged sperm motility and fertilization potential and that the sperm efficiently fertilized oocytes when reduced glutathione was added to the fertilization medium. Cryopreservation usually results in decreased sperm viability; an optimized protocol for cold storage of epididymides plus sperm cryopreservation has yet to be established. Here, we examined the motility and fertilization potential of cryopreserved, thawed (frozen-thawed) sperm from previously cold-stored mouse epididymides. We also examined the protective effect of sphingosine-1-phosphate (S1P) on sperm viability when S1P was added to the preservation medium during cold storage. We assessed viability of frozen-thawed sperm from mouse epididymides that had been cold-transported domestically or internationally and investigated whether embryos fertilized in vitro with these sperm developed normally when implanted in pseudo-pregnant mice. Our results indicate that frozen-thawed sperm from epididymides cold-stored for up to 48 hours maintained high fertilization potential. Fertilization potential was reduced after cold storage for 72 hours, but not if S1P was included in the cold storage medium. Live pups were born normally to recipients after in vitro fertilization using frozen-thawed sperm from cold-transported epididymides. In summary, we demonstrate an improved protocol for cold-storage of epididymides that can facilitate transport of genetically engineered-mice and preserve sperm viability after cryopreservation.

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Overview of new developments in and the future of cryopreservation in the laboratory mouse

et al. 2012

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The large-scale mutagenesis programmes underway around the world are generating thousands of novel GA mouse strains that need to be securely archived. In parallel with advances in mutagenesis, the procedures used to cryopreserve mouse stocks are being continually refined in order to keep pace with demand. Moreover, the construction of extensive research infrastructures for systematic phenotyping is fuelling demand for these novel strains of mice and new approaches to the distribution of frozen and unfrozen embryos and gametes are being developed in order to reduce the dependency on the transportation of live mice. This article highlights some contemporary techniques used to archive, rederive, and transport mouse strains around the world.

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Fertilization of C57BL/6 mouse sperm collected from cauda epididymides after preservation or transportation at 4°C using laser-microdissected oocytes

Cited by 25 publications

References 20 publications

Safety, efficacy and efficiency of laser-assisted IVF in subfertile mutant mouse strains

Safety, efficacy and efficiency of laser-assisted IVF in subfertile mutant mouse strains

Investigations of motility and fertilization potential in thawed cryopreserved mouse sperm from cold-stored epididymides

Overview of new developments in and the future of cryopreservation in the laboratory mouse

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