Ferulic acid inhibits LPS-induced apoptosis in bovine mammary epithelial cells by regulating the NF-κB and Nrf2 signalling pathways to restore mitochondrial dynamics and ROS generation

Liu, Mingjiang; Zhang, Chi; Xu, Xiaolong; Zhao, Xin; Han, Ziyi; Liu, Dandan; Bo, Ruonan; Li, Jingui; Liu, Zongping

doi:10.1186/s13567-021-00973-3

Cited by 32 publications

(21 citation statements)

References 47 publications

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“…Current studies have proved the importance of excessive ROS in inducing various cell apoptosis by the damage of lipids, proteins, and DNA in cells [ 53 , 54 ]. Our study demonstrated that the increase of ROS participated in LPS-induced apoptosis of BMECs, which was consistent with a previous study [ 46 , 55 ]. Moreover, excessive generation of ROS and the deficiency of antioxidants can result in the occurrence of oxidative stress which contributes to cell inflammation by inducing several signal molecules [ 38 ].…”

Section: Discussionsupporting

confidence: 94%

Selenium and Taurine Combination Is Better Than Alone in Protecting Lipopolysaccharide‐Induced Mammary Inflammatory Lesions via Activating PI3K/Akt/mTOR Signaling Pathway by Scavenging Intracellular ROS

Liu

Lin

et al. 2021

Oxidative Medicine and Cellular Longevity

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Mastitis is mainly induced by gram-negative bacterial infections, causing devastating economic losses to the global cattle industry. Both selenium (Se) and taurine (Tau) exhibit multiple biological effects, including reducing inflammation. However, no studies have reported the protective effect of the combined use of Se and Tau against mastitis, and the underlying mechanisms remain unclear. In this study, lipopolysaccharide (LPS), the vital virulence factor of gram-negative bacteria, was used to construct the in vivo and vitro mastitis models. The results of in vivo model showed that Se and Tau combination was more effective than either substance alone in reducing tissue hyperemia, edema, and neutrophil infiltration in the mammary acinar cavity, improving the blood-milk barrier in LPS-induced mice mastitis, and decreasing the expression of proinflammatory factors and the activity of MPO. Moreover, Se and Tau combination significantly increased the levels of LPS-induced reduction in PI3K/Akt/mTOR, but the expressions of TLRs and NLRP3 were not significantly changed in the mammary tissue. In the in vitro experiments, the effects of Se and Tau combination or alone on inflammatory factors, inflammatory mediators, MPO activity, and blood-milk barrier were consistent with those in vivo. The Se and Tau combination has also been found to increase the survival rate of BMECs compared with each substance alone via promoting cellular proliferation and inhibiting apoptosis. Also, it has been confirmed that this combination could restore the LPS-induced inhibition in the PI3K/Akt/mTOR signaling pathway. Inhibition of mTOR by Rapamycin counteracted the combined protection of SeMet and Tau against LPS-induced inflammatory damage, the inhibition of PI3K by LY294002 blocked the activation of mTOR, and the accumulation of ROS by the ROS agonist blocked the activation of PI3K. In conclusion, these findings suggested that Se and Tau combination was better than either substance alone in protecting LPS-induced mammary inflammatory lesions by upregulating the PI3K/Akt/mTOR signaling pathway.

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Section: Discussionsupporting

confidence: 94%

Selenium and Taurine Combination Is Better Than Alone in Protecting Lipopolysaccharide‐Induced Mammary Inflammatory Lesions via Activating PI3K/Akt/mTOR Signaling Pathway by Scavenging Intracellular ROS

Liu

Lin

et al. 2021

Oxidative Medicine and Cellular Longevity

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“…In the study, LPS stimulated RAW264.7 cells to produce ROS at 3 h. DE reduced ROS production at 3 h of LPS stimulation. The nuclear transcription factor Nrf2 has been reported to play an important regulatory role in ROS production, and Nrf2 activity is inhibited at the very beginning of LPS stimulation of cells before it is activated ( Liu et al, 2021 ). ROS production is likely to be regulated by Nrf2, with early LPS stimulation resulting in decreased Nrf2 production and increased ROS production, and as time passes, Nrf2 production rises and ROS production declines.…”

Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory Effects and Mechanisms of Dandelion in RAW264.7 Macrophages and Zebrafish Larvae

Luo

et al. 2022

Front. Pharmacol.

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Dandelions (Taraxacum spp.) play an important role in the treatment of inflammatory diseases. In this study, we investigated the anti-inflammatory effects of Dandelion Extract (DE) in LPS-induced RAW264.7 macrophages and copper sulfate (CuSO4)-induced zebrafish larvae. DE was not toxic to RAW264.7 cells at 75 μg/ml as measured by cell viability, and DE inhibited LPS-induced cell morphological changes as measured by inverted microscopy. In survival experiments, DE at 25 μg/ml had no toxicity to zebrafish larvae. By using an enzymatic standard assay, DE reduced the production of nitric oxide (NO) in LPS-induced RAW264.7 cells. Fluorescence microscopy results show that DE reduced LPS-induced ROS production and apoptosis in RAW264.7 cells. DE also inhibited CuSO4-induced ROS production and neutrophil aggregation in zebrafish larvae. The results of flow cytometry show that DE alleviated the LPS-induced cell cycle arrest. In LPS-induced RAW264.7 cells, RT-PCR revealed that DE decreased the expression of M1 phenotypic genes iNOS, IL-6, and IL-1β while increasing the expression of M2 phenotypic genes IL-10 and CD206. Furthermore, in CuSO4-induced zebrafish larvae, DE reduced the expression of iNOS, TNF-α, IL-6, and IL-10. The findings suggest that DE reduces the LPS-induced inflammatory response in RAW264.7 cells by regulating polarization and apoptosis. DE also reduces the CuSO4-induced inflammatory response in zebrafish larvae.

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“…In mastitis, the release of ROS is key to the inflammatory response [ 45 ]. On the one hand, ROS plays an important role in inflammation, apoptosis, and cell growth [ 46 , 47 ]. On the other hand, ROS can cause oxidation of proteins and DNA, inducing damage to nearby tissues.…”

Section: Discussionmentioning

confidence: 99%

N6-Methyladenosine Modification Profile in Bovine Mammary Epithelial Cells Treated with Heat-Inactivated Staphylococcus aureus

Zhu

Lin

et al. 2022

Oxidative Medicine and Cellular Longevity

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The symptoms of mastitis caused by Staphylococcus aureus (S. aureus) in dairy cows are not obvious and difficult to identify, resulting in major economic losses. N6-Methyladenosine (m6A) modification has been reported to be closely associated with the occurrence of many diseases. However, only a few reports have described the role of m6A modification in S. aureus-induced mastitis. In this study, after 24 h of treatment with inactivated S. aureus, MAC-T cells (an immortalized bovine mammary epithelial cell line) showed increased expression levels of the inflammatory factors IL-1β, IL-6, TNF-α, and reactive oxygen species. We found that the mRNA levels of METLL3, METLL14, WTAP, and ALKBH5 were also upregulated. Methylated RNA immunoprecipitation sequencing analysis revealed that 133 genes were m6A hypermethylated, and 711 genes were m6A hypomethylated. Biological functional analysis revealed that the differential m6A methylated genes were mainly related to oxidative stress, lipid metabolism, inflammatory response, and so on. In the present study, we also identified 62 genes with significant changes in m6A modification and mRNA expression levels. These findings elucidated the m6A modification spectrum induced by S. aureus in MAC-T cells and provide the basis for subsequent m6A research on mastitis.

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Ferulic acid inhibits LPS-induced apoptosis in bovine mammary epithelial cells by regulating the NF-κB and Nrf2 signalling pathways to restore mitochondrial dynamics and ROS generation

Cited by 32 publications

References 47 publications

Selenium and Taurine Combination Is Better Than Alone in Protecting Lipopolysaccharide‐Induced Mammary Inflammatory Lesions via Activating PI3K/Akt/mTOR Signaling Pathway by Scavenging Intracellular ROS

Selenium and Taurine Combination Is Better Than Alone in Protecting Lipopolysaccharide‐Induced Mammary Inflammatory Lesions via Activating PI3K/Akt/mTOR Signaling Pathway by Scavenging Intracellular ROS

Anti-Inflammatory Effects and Mechanisms of Dandelion in RAW264.7 Macrophages and Zebrafish Larvae

N6-Methyladenosine Modification Profile in Bovine Mammary Epithelial Cells Treated with Heat-Inactivated Staphylococcus aureus

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