FGF8 is Essential for Functionality of Induced Neural Precursor Cell-derived Dopaminergic Neurons

Lim, Mi-Sun; Lee, Soo Young; Park, Chang-Hwan

doi:10.15283/ijsc.2015.8.2.228

Cited by 16 publications

(12 citation statements)

References 20 publications

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“…This approach includes trans-differentiation to a desired cell type from autologous cells ( Graf and Enver, 2009 ; Lee et al ., 2015 ). For example, many papers achieved trans-differentiation of fibroblasts to functional and specific cell types such as neurons, neural precursor cells (NPC), astrocytes, oligodendrocytes, cardiomyocytes, and hepatocytes ( Ieda et al ., 2010 ; Vierbuchen et al ., 2010 ; Sekiya and Suzuki, 2011 ; Caiazzo et al ., 2015 ; Lim et al ., 2015a , 2015b ; Prasad et al ., 2017 ). Important factors in trans-differentiation include the efficiency of reprogramming and suitability for experimental implementation.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Reprogramming Methods for Generation of Induced-Oligodendrocyte Precursor Cells

Lee

Park

2017

Biomolecules & Therapeutics

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Direct conversion by trans-differentiation is of growing interest in cell therapy for incurable diseases. The efficiency of cell reprogramming and functionality of converted cells are important considerations in cell transplantation therapy. Here, we compared two representative protocols for the generation of induced-oligodendrocyte progenitor cells (iOPCs) from mouse and rat fibroblasts. Then, we showed that induction of Nkx6.2, Olig2, and Sox10 (NOS) was more effective in mouse fibroblasts and that induction of Olig2, Sox10, and Zfp536 (OSZ) was more effective at reprogramming iOPCs from rat fibroblasts. However, OSZ-iOPCs did not show greater proliferation than NOS-induced cells. Because the efficiency of iOPCs generation appears to differ between cell species depending on transcription factors and culture conditions, it is important to select appropriate methods for efficient reprogramming.

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Section: Introductionmentioning

confidence: 99%

Comparison of Reprogramming Methods for Generation of Induced-Oligodendrocyte Precursor Cells

Lee

Park

2017

Biomolecules & Therapeutics

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“…Data are expressed as mean±SEM. When more than two groups were compared, a paired t -test was performed using SigmaPlot for Windows, version 10.0 (Systat Software GmbH, Erkrath, Germany) (5).…”

Section: Methodsmentioning

confidence: 99%

“…The direct differentiation of somatic cells into tissuespecific cells such as induced neurons (1) and neural precursor cells (2–5) may serve as an attractive approach in the field of stem cell research. The first study in this direction was reported by Vierbuchen et al (1) using neuronal lineage-inducing transcription factors such as Brn2 , Ascl1 , and Myt1L (BAM).…”

Section: Introductionmentioning

confidence: 99%

Hybrid Nanofiber Scaffold-Based Direct Conversion of Neural Precursor Cells/Dopamine Neurons

Lim

Kim

et al. 2019

IJSC

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The concept of cellular reprogramming was developed to generate induced neural precursor cells (iNPCs)/dopaminergic (iDA) neurons using diverse approaches. Here, we investigated the effects of various nanoscale scaffolds (fiber, dot, and line) on iNPC/iDA differentiation by direct reprogramming. The generation and maturation of iDA neurons (microtubule-associated protein 2-positive and tyrosine hydroxylase-positive) and iNPCs (NESTIN-positive and SOX2-positive) increased on fiber and dot scaffolds as compared to that of the flat (control) scaffold. This study demonstrates that nanotopographical environments are suitable for direct differentiation methods and may improve the differentiation efficiency.

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“…FGF-8 is also a promising candidate for the treatment of neurodegenerative diseases, where it has broad activity in neural tissue and is vital in promoting dopaminergic neuron development and function ( Chen et al, 2016 ). FGF-8 can induce dopaminergic neuronal differentiation and promote dopaminergic axons’ growth in the midbrain by increasing Semaphorin 3F ( Yamauchi et al, 2009 ; Lim et al, 2015 ). Two major FGF-8 isoforms are expressed in the midbrain (FGF-8a and FGF-8b), whereas FGF-8 b promotes midbrain development, and FGF-8b is structurally similar to FGF-18 and has similar receptor-binding characteristics ( Liu et al, 2003 ).…”

Section: Fibroblast Growth Factor Plays An Essential Role In Protecting and Repairing Dopaminergic Neuronsmentioning

confidence: 99%

FGF, Mechanism of Action, Role in Parkinson’s Disease, and Therapeutics

et al. 2021

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Parkinson’s disease (PD) is a neurodegenerative disease associated with severe disability and adverse effects on life quality. In PD, motor dysfunction can occur, such as quiescence, muscle stiffness, and postural instability. PD is also associated with autonomic nervous dysfunction, sleep disorders, psychiatric symptoms, and other non-motor symptoms. Degeneration of dopaminergic neurons in the substantia nigra compact (SNPC), Lewy body, and neuroinflammation are the main pathological features of PD. The death or dysfunction of dopaminergic neurons in the dense part of the substantia nigra leads to dopamine deficiency in the basal ganglia and motor dysfunction. The formation of the Lewy body is associated with the misfolding of α-synuclein, which becomes insoluble and abnormally aggregated. Astrocytes and microglia mainly cause neuroinflammation, and the activation of a variety of pro-inflammatory transcription factors and regulatory proteins leads to the degeneration of dopaminergic neurons. At present, PD is mainly treated with drugs that increase dopamine concentration or directly stimulate dopamine receptors. Fibroblast growth factor (FGF) is a family of cellular signaling proteins strongly associated with neurodegenerative diseases such as PD. FGF and its receptor (FGFR) play an essential role in the development and maintenance of the nervous system as well as in neuroinflammation and have been shown to improve the survival rate of dopaminergic neurons. This paper summarized the mechanism of FGF and its receptors in the pathological process of PD and related signaling pathways, involving the development and protection of dopaminergic neurons in SNPC, α-synuclein aggregation, mitochondrial dysfunction, and neuroinflammation. It provides a reference for developing drugs to slow down or prevent the potential of PD.

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FGF8 is Essential for Functionality of Induced Neural Precursor Cell-derived Dopaminergic Neurons

Cited by 16 publications

References 20 publications

Comparison of Reprogramming Methods for Generation of Induced-Oligodendrocyte Precursor Cells

Comparison of Reprogramming Methods for Generation of Induced-Oligodendrocyte Precursor Cells

Hybrid Nanofiber Scaffold-Based Direct Conversion of Neural Precursor Cells/Dopamine Neurons

FGF, Mechanism of Action, Role in Parkinson’s Disease, and Therapeutics

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