Four reinforced concrete rooms are used in this study, with varying floor areas, openings, and fuel loads. These rooms are office, Classroom, computer, and chemical laboratories of institutional buildings. The time versus Eurocode (EC) parametric fire relationship is determined and compared with ISO 834 and ASTM 119. The performance of the slab is evaluated based on the EC target reliability index of 3.8 for 50 years design period using the First Order Reliability Method (FORM). The probability of failure and structural reliability of each fire compartment at different fire duration is determined. Variables used were concrete compressive strength, a reduction factor of concrete compressive strength, steel yield strength, concrete cover, slab depth, unit width, and area of steel reinforcement provided. The reliability is determined at each 30 minutes fire duration for all fire compartments. The starting point is ambient temperature, then after every thirty minutes until the time when the maximum gas temperature is reached. The fire compartments were able to show fire resistance for two hours and then started to drop over time. Yield strength reduction factor, concrete cover, and area of reinforcement respectively were the most significant variables to determine the reliability index. The structure was able to withhold fire until two hours with a reliability of more than 3.8after that, the slab reliability was less than the target reliability and will need a repair.