Fibrin in Tissue Engineering

Endochondral Ossification for Enhancing Bone Regeneration: Converging Native Extracellular Matrix Biomaterials and Developmental Engineering In Vivo

“…46,47 As a result, many regenerative design strategies have focused on isolating and concentrating portions of human blood such as platelets and plasma (e.g., platelet-rich plasma [PRP]) or pro-coagulation molecules (e.g., fibrin sealants) to enhance endogenous healing responses. [48][49][50][51][52][53][54] It remains to be seen whether a scaffold with only inflammation-related chemical and mechanical cues can elicit enough of an EC ossification response to heal critical-sized bone defects. Pure fibrin scaffolds and PRP alone do not appear to exhibit sufficient cartilage [48][49][50]52 and bone 51,53,54 regeneration in critical-sized defects, which suggests that additional regenerative stimuli such as bioactive molecules and progenitor stem cells may be required.…”

An In Vitro Model for the Development of Mature Bone Containing an Osteocyte Network

“…[17,24,26] Following isolation, periosteum cells were seeded into fibrin hydrogels, which critically permit selfassembly and imaging of a de novo collagen matrix, and because as materials, fibrin scaffolds have structural and biochemical similarities to the microenvironment of the callus formed early in fracture healing. [27][28][29] Both cortical and trabecular bone show a similar matrix alignment at the microscale, which is essential for development and mechanical resistance. To mimic this important feature, the environment of the fracture callus was further emulated by introducing two calcium phosphate (CaP) anchors at the extremities of the culture dish.…”

Stacked stem cell sheets enhance cell-matrix interactions

“…Fibrin, as a nature biomaterial, has been widely used in tissue engineering applications. [12][13][14] When applied as a cardiac patch to treat MI, fibrin has been shown to provide temporary physical support of the infarct tissue and prevent negative left ventricular remodeling. 15,16 The investigation on…”