Fibrinogen Measurements in Plasma and Whole Blood

Ogawa, Satoshi; Tanaka, Kenichi A.; Nakajima, Yasufumi; Nakayama, Yushi; Takeshita, Jun; Arai, M.; Mizobe, Toshiki

doi:10.1213/ane.0000000000000448

Cited by 43 publications

(3 citation statements)

References 31 publications

(26 reference statements)

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“…Other techniques for measuring fibrinogen include Prothrombin-time (PT) derived tests, immunohematological assays (11), the dry-hematology method (21), and thrombin generation assays. These tests are not widely used or only available in research settings (22).…”

Section: Fibrinogen Evaluation and Specific Pitfalls In The Pediatric Settingmentioning

confidence: 99%

Pediatric Fibrinogen PART I—Pitfalls in Fibrinogen Evaluation and Use of Fibrinogen Replacement Products in Children

Huisman

Crighton

2021

Front. Pediatr.

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Fibrinogen is a key coagulation protein, playing a critical role in hemostasis. It is the first factor to decrease to critical levels during bleeding. Hypofibrinogenemia is an important risk factor for bleeding in clinical settings, including pediatric surgery. Yet, the optimal measurement of fibrinogen levels is subject to debate, as is the critical threshold for intervention. Fibrinogen replacement may be provided by cryoprecipitate and fibrinogen concentrate. Whilst both products contain fibrinogen, they are not equivalent, each has its own advantages and disadvantages, especially for pediatric use. Unfortunately, medical literature to support fibrinogen replacement in children is limited. In this article we review the current diagnostic tools to measure fibrinogen, with respect to their use in the pediatric critical care setting. Secondly, we evaluate the different fibrinogen replacement therapies, focusing on cryoprecipitate and fibrinogen concentrate and examine their individual product characteristics, associated risks and benefits, different dosing strategies and specific pitfalls for use in children. We summarize by highlighting current knowledge gaps and areas for future research.

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Section: Fibrinogen Evaluation and Specific Pitfalls In The Pediatric Settingmentioning

confidence: 99%

Pediatric Fibrinogen PART I—Pitfalls in Fibrinogen Evaluation and Use of Fibrinogen Replacement Products in Children

Huisman

Crighton

2021

Front. Pediatr.

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“…Among them, viscoelastic hemostasis and DRIHEMATO have been shown to detect fibrinogen in whole blood with high sensitivity. 24 However, it is worth noting that both these methods require expensive equipment that is difficult to transport. Paper-based biosensors have made significant progress in the past few years.…”

Section: Introductionmentioning

confidence: 99%

A point-of-care microfluidic channel-based device for rapid and direct detection of fibrinogen in whole blood

Ban¹,

Li²,

Cao³

et al. 2022

Lab Chip

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“…Bleeding and clotting disorders cause extensive morbidity and mortality, especially in resource-poor settings, which may lack access to suitable testing methods. There are several routine hospital-based methods for fibrinogen in whole blood and plasma including the Clauss assay, 5 viscoelastic assays, 6 , 7 dry haematology methods, 8 and prothrombin time-derived fibrinogen assay. 5 However, such approaches are not suitable for point-of-care use in resource-limited environments.…”

mentioning

confidence: 99%

Paper-Based Lateral Flow Device for the Sustainable Measurement of Human Plasma Fibrinogen in Low-Resource Settings

et al. 2021

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Fibrinogen concentration is a major determinant of both clotting and bleeding risk. Clotting and bleeding disorders cause extensive morbidity and mortality, particularly in resource-poor and emergency settings. This is exacerbated by a lack of timely intervention informed by measurement of fibrinogen levels under conditions such as thrombosis or postpartum haemorrhage. There is an absence of simple, rapid, low-cost, and sustainable diagnostic devices for fibrinogen measurement that can be deployed in such environments. Paper-based analytical devices are of significant interest due to their potential for low-cost production, ease of use, and environmental sustainability. In this work, a device for measuring blood plasma fibrinogen using chromatography paper was developed. Wax printing was used to create hydrophobic structures to define the test channel and sample application zone. Test strips were modified with bovine thrombin. Plasma samples (22 μL) were applied, and the flow rate was monitored over 5 min. As the sample traversed the strip, clotting was induced by the conversion of soluble fibrinogen to insoluble fibrin. The flow rate and distance travelled by the sample were dependent on fibrinogen concentration. The device was able to measure fibrinogen concentration in the range of 0.5–7.0 ± 0.3 mg/mL ( p < 0.05, n = 24) and had excellent correlation with laboratory coagulometry in artificial samples ( r 2 = 0.9582, n = 60). Devices were also stable at 4–6 °C for up to 3 weeks.

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Fibrinogen Measurements in Plasma and Whole Blood

Cited by 43 publications

References 31 publications

Pediatric Fibrinogen PART I—Pitfalls in Fibrinogen Evaluation and Use of Fibrinogen Replacement Products in Children

Pediatric Fibrinogen PART I—Pitfalls in Fibrinogen Evaluation and Use of Fibrinogen Replacement Products in Children

A point-of-care microfluidic channel-based device for rapid and direct detection of fibrinogen in whole blood

Paper-Based Lateral Flow Device for the Sustainable Measurement of Human Plasma Fibrinogen in Low-Resource Settings

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