Fibronectin Adsorption, Cell Adhesion, and Proliferation on Nanostructured Tantalum Surfaces

Dolatshahi-Pirouz, Alireza; Jensen, Tonny; Kraft, David C.; Foss, Morten; Kingshott, Peter; Hansen, J. Lundsgaard; Larsen, A. Nylandsted; Chevallier, J.; Besenbacher, Flemming

doi:10.1021/nn9017872

Cited by 170 publications

(138 citation statements)

References 65 publications

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“…22 The formation of focal adhesion contacts and cell adhesion is an important starting point for cell proliferation, differentiation, and some cellular behaviors, which is activated by the formation of vinculin adhesion spots. 23,24 The higher expression exhibited better adhesion. It has been reported that various kinds of physical stresses can accelerate stem cell differentiation into specific cells.…”

mentioning

confidence: 99%

Optimizing stem cell functions and antibacterial properties of TiO2 nanotubes incorporated with ZnO nanoparticles: experiments and modeling

Liu¹,

Su²,

Gonzales³

et al. 2015

IJN

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To optimize mesenchymal stem cell differentiation and antibacterial properties of titanium (Ti), nano-sized zinc oxide (ZnO) particles with tunable concentrations were incorporated into TiO 2 nanotubes (TNTs) using a facile hydrothermal strategy. It is revealed here for the first time that the TNTs incorporated with ZnO nanoparticles exhibited better biocompatibility compared with pure Ti samples (controls) and that the amount of ZnO (tailored by the concentration of Zn(NO 3 ) 2 in the precursor) introduced into TNTs played a crucial role on their osteogenic properties. Not only was the alkaline phosphatase activity improved to about 13.8 U/g protein, but the osterix, collagen-I, and osteocalcin gene expressions was improved from mesenchymal stem cells compared to controls. To further explore the mechanism of TNTs decorated with ZnO on cell functions, a response surface mathematical model was used to optimize the concentration of ZnO incorporation into the Ti nanotubes for stem cell differentiation and antibacterial properties for the first time. Both experimental and modeling results confirmed ( R 2 values of 0.8873–0.9138 and 0.9596–0.9941, respectively) that Ti incorporated with appropriate concentrations (with an initial concentration of Zn(NO 3 ) 2 at 0.015 M) of ZnO can provide exceptional osteogenic properties for stem cell differentiation in bone cells with strong antibacterial effects, properties important for improving dental and orthopedic implant efficacy.

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mentioning

confidence: 99%

Optimizing stem cell functions and antibacterial properties of TiO2 nanotubes incorporated with ZnO nanoparticles: experiments and modeling

Liu¹,

Su²,

Gonzales³

et al. 2015

IJN

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“…A typical review, focusing on 2D nanotopographical surfaces, highlighted that cells are in some cases essentially controlled by the surface-adsorbed proteins rather than by direct signaling from nanoscale surface cues. 23 However, recent studies found that the adsorbed fibronectin did not affect cell adhesion on nano-hut/dome tantalum surfaces compared to flat surfaces, 14 and the reduced cell adhesion on TiC nanowires 18 and SiO 2 nanorods 19 was independent of protein adsorption. These findings imply that the process of cell adhesion on 3D nanotopographs may be directly mediated by signaling from nanoscale surface cues rather than by surface-adsorbed proteins, although there was lack of evidence in the absence of serum.…”

Section: Introductionmentioning

confidence: 95%

“…8 Cell-material interactions depend on the surface properties of the material, such as chemistry, 2,9-11 surface energy, 2,12 roughness, 13 and topography. [14][15][16][17][18][19][20][21][22] Recent works demonstrated that three-dimensional (3D) nanotopography could influence cell adhesion; however, conflicting results have been reported. For example, hydroxyapatite (HA) nanoneedles and nanofibers, 17 quasi-aligned TiC and TiO 2 nanowires, 18 and randomly oriented and upright SiO 2 and ZnO nanorods 19 inhibited adhesion and spreading of cells.…”

Section: Introductionmentioning

confidence: 99%

Direct role of interrod spacing in mediating cell adhesion on Sr-HA nanorod-patterned coatings

Zhou¹,

Han²,

Lu³

2014

IJN

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The process in which nanostructured surfaces mediate cell adhesion is not well understood, and may be indirect (via protein adsorption) or direct. We prepared Sr-doped hydroxyapatite (Sr 1 -HA) 3D nanorods (with interrod spacing of 67.3±3.8, 95.7±4.2, and 136.8±8.7 nm) and 2D nanogranulate patterned coatings on titanium. Employing the coatings under the same surface chemistry and roughness, we investigated the indirect/direct role of Sr 1 -HA nanotopographies in the regulation of osteoblast adhesion in both serum-free and serum-containing Dulbecco’s Modified Eagle/Ham’s Medium. The results reveal that the number of adherent cells, cell-secreted anchoring proteins (fibronectin, vitronectin, and collagen), vinculin and focal adhesion kinase (FAK) denoted focal adhesion (FA) contact, and gene expression of vinculin, FAK, and integrin subunits (α 2 , α 5 , α v , β 1 , and β 3 ), undergo significant changes in the inter-nanorod spacing and dimensionality of Sr 1 -HA nanotopographies in the absence of serum; they are significantly enhanced on the <96 nm spaced nanorods and more pronounced with decreasing interrod spacing. However, they are inhibited on the >96 nm spaced nanorods compared to nanogranulated 2D topography. Although the adsorption of fibronectin and vitronectin from serum are higher on 136.8±8.7 nm spaced nanorod patterned topography than nanogranulated topography, cell adhesion is inhibited on the former compared to the latter in the presence of serum, further suggesting that reduced cell adhesion is independent of protein adsorption. It is clearly indicated that 3D nanotopography can directly modulate cell adhesion by regulating integrins, which subsequently mediate anchoring proteins’ secretion and FA formation rather than via protein adsorption.

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“…It was documented that the surface fibronectin adsorption is directly related to cell functions. 29 Moreover, our plasma Ti-coated samples required no chemical etchants or subsequent postprocessing. The treatment method uses a Ti plasma to modify the surface, thereby eliminating the possibility of metal contaminants.…”

Section: Plasma Nanocoating For Improved Stem Cell Functionsmentioning

confidence: 99%

Enhanced human bone marrow mesenchymal stem cell functions on cathodic arc plasma-treated titanium

Zhu¹,

Teel²,

O’Brien³

et al. 2015

IJN

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Surface modification of titanium for use in orthopedics has been explored for years; however, an ideal method of integrating titanium with native bone is still required to this day. Since human bone cells directly interact with nanostructured extracellular matrices, one of the most promising methods of improving titanium’s osseointegration involves inducing bio-mimetic nanotopography to enhance cell–implant interaction. In this regard, we explored an approach to functionalize the surface of titanium by depositing a thin film of textured titanium nanoparticles via a cathodic arc discharge plasma. The aim is to improve human bone marrow mesenchymal stem cell (MSC) attachment and differentiation and to reduce deleterious effects of more complex surface modification methods. Surface functionalization was analyzed by scanning electron microscopy, atomic force microscopy, contact angle testing, and specific protein adsorption. Scanning electron microscopy and atomic force microscopy examination demonstrate the deposition of titanium nanoparticles and the surface roughness change after coating. The specific fibronectin adsorption was enhanced on the modified titanium surface that associates with the improved hydrophilicity. MSC adhesion and proliferation were significantly promoted on the nanocoated surface. More importantly, compared to bare titanium, greater production of total protein, deposition of calcium mineral, and synthesis of alkaline phosphatase were observed from MSCs on nanocoated titanium after 21 days. The method described herein presents a promising alternative method for inducing more cell favorable nanosurface for improved orthopedic applications.

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Fibronectin Adsorption, Cell Adhesion, and Proliferation on Nanostructured Tantalum Surfaces

Cited by 170 publications

References 65 publications

Optimizing stem cell functions and antibacterial properties of TiO2 nanotubes incorporated with ZnO nanoparticles: experiments and modeling

Optimizing stem cell functions and antibacterial properties of TiO2 nanotubes incorporated with ZnO nanoparticles: experiments and modeling

Direct role of interrod spacing in mediating cell adhesion on Sr-HA nanorod-patterned coatings

Enhanced human bone marrow mesenchymal stem cell functions on cathodic arc plasma-treated titanium

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