Fibronectin–calcium phosphate composite layer on hydroxyapatite to enhance adhesion, cell spread and osteogenic differentiation of human mesenchymal stem cellsin vitro

Abstract

Fibronectin (Fn) and type I collagen (Col) were immobilized on a surface of a hydroxyapatite (HAP) ceramic by coprecipitation with calcium phosphate in a supersaturated calcium phosphate solution prepared by mixing clinically approved infusion fluids. These proteins and the calcium phosphate precipitate formed a composite surface layer. As a result, the proteins were immobilized firmly as not to be released completely for 3 d in a physiological salt solution. When human mesenchymal stem cells (hMSCs) were cultured on a HAP ceramic in a differentiation medium supplemented with dexamethasone, beta-glycerophosphate and ascorbic acid, hMSCs spread well within 1 h. The alkaline phosphatase (ALP) activity of hMSCs cultured on the Fn-calcium phosphate composite layer significantly increased compared with that of hMSCs cultured on the untreated HAP ceramic. On the other hand, Col did not increase the ALP activity of hMSCs and no synergy between Fn and Col was observed. Therefore, the Fn-calcium phosphate composite layer formed on the HAP is useful for the enhancement of the spreading and osteogenic differentiation of hMSCs in vitro.

Calcium orthophosphate-based biocomposites and hybrid biomaterials

“…silk fibroin [726], fibronectin [727], and casein phosphopeptides [728]. Besides, the reader's attention is pointed out to an interesting approach to crystallize CDHA inside poly(allylamine)/poly(styrene sulfonate) polyelectrolyte capsules resulting in empty biocomposite spheres of micron size [729].…”

Effects of histone deacetylase inhibitors on regenerative cell responses in human dental pulp cells

“…, Sogo et al . ). Furthermore, NF‐κB‐induced upregulation of adhesion molecules, such as ICAM‐1, VCAM‐1 and E‐selectin, is reportedly key to many cell migratory‐regulated events (Koh et al .…”

Umbilical cord stem cells released from alginate–fibrin microbeads inside macroporous and biofunctionalized calcium phosphate cement for bone regeneration

“…In contrast, the cells released from the microbeads attached to CPC, showed a healthy spreading morphology, and quickly proliferated. Furthermore, in previous studies, Fn and RGD were used to promote cell adhesion to biomaterials including pre-formed calcium phosphates [42,43]. However, there has been no report on incorporating Fn and RGD into CPC.…”