Field Performance and Genetic Diversity of Chickpea Genotypes

Abstract

Chickpea (Cicer arietinum L.) is an important legume crop widely grown across the globe. In this study, twenty six chickpea genotypes, of diverse origin, were evaluated for field performance and genetic diversity. Significant differences in morphological characters of tested chickpea genotypes were observed at vegetative and reproductive stages under field conditions. Amplified fragments length polymorphism (AFLP) and sequence-related amplified polymorphism (SRAP) markers, used in this study, showed considerable genetic diversity among the tested genotypes. With markers, 716 AFLP and 1164 polymorphic SRAP loci were analyzed using four AFLP and six SRAP primer combinations. The values for polymorphism information content for SRAP and AFLP were more than 0.6 and 0.9, respectively. In addition, many subclusters within genotypes were noted, which indicated high diversity level in the tested genotypes. Clustering of chickpea genotypes was largely based on genetic background and/or origin. Both morphological and molecular data helped to quantify the genetic diversity in tested chickpea genotypes and may be useful for the use in breeding program aimed at yield improvement.

Genetic relationship of mungbean and blackgram genotypes based on agronomic and photosynthetic performance and SRAP markers

“…This coherence between SRAP and morphological pattern has also been reported in other legume crops, i.e. pea and chickpea (Espósito et al, 2009;Khan et al, 2016).…”

Biochemical and molecular characterization of cowpea landraces using seed storage proteins and SRAP marker patterns

“…SRAP marker characteristics include: a reasonable throughput rate, disclosure of numerous co-dominant markers, more reproducible than RAPDs and ease of performing assays compared to AFLPs and, most importantly, targeting of open reading frames (ORFs). In legumes SRAP was used to assess the genetic diversity in faba beans (Alghamdi et al, 2012, Ammar et al, 2015), lentils (Rana et al, 2009, Alghamdi et al, 2014), alfalfa (Vandemark et al, 2006, Ariss and Vandemark, 2007, Castonguay et al, 2010, Al-Faifi et al, 2013), peas (Esposito et al, 2007, Guindon et al, 2016) and chickpeas (Khan et al, 2016). However, SRAP markers have rarely been applied to assess genetic variation of cowpea, and to our knowledge, this is the first application of SRAP markers used for assessment of genetic variability in cowpeas.…”

Estimation of genetic diversity among faba bean accessions based on agro-morphological characters using multivariate analysis technique

“…In another study, Sharifi (2015) opined that yield contributing traits should be considered in faba bean breeding programs as selection criteria. Collecting high-yielding accessions can further leverage this important correlation (Afzal et al, 2018).…”