Field transplantation of a freshwater bivalve, <i>Pyganodon grandis</i>, across a metal contamination gradient. I. Temporal changes in metallothionein and metal (Cd, Cu, and Zn) concentrations in soft tissues

Couillard, Yves; Campbell, Peter G. C.; Tessier, André; Auclair, J. L.; Pellerin-Massicotte, Jocelyne

doi:10.1139/f95-070

Cited by 69 publications

(46 citation statements)

References 17 publications

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“…Thus, in response to Ni exposure, the copepod could induce MT synthesis to act on the detoxification function and thereafter counteract metal attack. It should be noted that the MT level measured in organisms is actually a result of the interaction between the synthesis and degradation process (Couillard et al 1995). It could be assumed that the copepod T. japonicus might promptly initiate the detoxification process involving MT as a response to Ni exposure, and the increase in MT activity might result in more turnover of this protein, but not in an enhanced content, which is also demonstrated by Barka et al (2001).…”

Section: Discussionmentioning

confidence: 90%

Oxidative damage effects in the copepod Tigriopus japonicus Mori experimentally exposed to nickel

Wang

2009

Ecotoxicology

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Tigriopus japonicus Mori has been recognized as a good model for toxicological testing of marine pollutants. Recently, a large number of genes have been identified from this copepod, and their mRNA expression has been studied independently against exposure to marine pollutants; however, biochemical-response information is relatively scarce. The response of T. japonicus to nickel (Ni) additions was examined under laboratory-controlled conditions in 12 days exposure. Superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione-S-transferase (GST), acetylcholinesterase (AchE), reduced glutathione (GSH), the ratio of reduced to oxidized glutathione (GSH/GSSG) and metallothionein (MT) were analyzed for Ni treatments (0, 0.125, 0.25, 0.75 and 3.0 mg/L) after 1, 4, 7 and 12 days. The thiobarbituric reactive species assay was used to evaluate lipid peroxidation (LPO) level in copepods after exposure. The results showed that Ni remarkably affected the biochemical parameters (SOD, GPx, GST, GSH, and GSH/GSSG) after certain exposure durations. However, the copepod's LPO level was significantly decreased under metal treatments after exposure, hinting that the factors involved in LPO might not significantly depend on the operations and functions in the antioxidant system. Ni exhibited the neurotoxicity to copepods, because its use obviously elevated AchE activity. During exposure, Ni initially displayed an inhibition effect but induced MT synthesis in T. japonicus by day 12, probably being responsible for metal detoxification. Thus, Ni had intervened in the detoxification process and antioxidant system of this copepod, and it could be used as a suitable bioindicator of Ni exposure via measuring SOD, GPx, GST, and MT as biomarkers.

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Section: Discussionmentioning

confidence: 90%

Oxidative damage effects in the copepod Tigriopus japonicus Mori experimentally exposed to nickel

Wang

2009

Ecotoxicology

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“…This study was conducted primarily in the mining region of Abitibi, northwestern Quebec, and includes five lakes along a metal contamination gradient (Couillard et al 1995). Relative metal pollution of these lakes is indicated in Fig.…”

Section: Study Sitesmentioning

confidence: 99%

Bioenergetic costs of heavy metal exposure in yellow perch (Perca flavescens): in situ estimates with a radiotracer (¹³⁷Cs) technique

Sherwood¹,

Rasmussen²,

Rowan³

et al. 2000

Can. J. Fish. Aquat. Sci.

103

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While the flow of energy is understood to determine the growth of organisms and the productivity of ecosystems, little is known about the sublethal effect of pollutants on the energetic efficiency of wild populations. We used field estimates of fish growth coupled to in situ estimates of food consumption rates obtained from the mass balance of a globally dispersed, trophically transferred radiotracer ( 137 Cs) to demonstrate the bioenergetic impairment of yellow perch (Perca flavescens) from lakes polluted by heavy metals (Cd, Cu, and Zn). Annual growth increment relative to the total energy budget (conversion efficiency) was about three times lower in cortisol-impaired yellow perch from metal-polluted lakes relative to yellow perch from reference lakes (4.2% compared with 10.8%), suggesting that fish exposed to pollutants experienced greater total energetic costs. In addition, metal-polluted lakes were dominated by adult yellow perch populations and simplified prey bases, suggesting that effects are occurring at multiple levels of biological organization. Our in situ bioenergetic approach to toxicity assessment provides a measurable and ecologically relevant endpoint for assessing the sublethal effects of pollutants on fish communities.Résumé : Bien qu'on sache que le flux d'énergie détermine la croissance des organismes et la productivité des écosys-tèmes, on sait peu de choses de l'effet sublétal des polluants sur l'efficacité énergétique des populations sauvages. Nous avons utilisé des estimations de la croissance des poissons dans la nature couplées à des estimations des taux d'alimentation in situ obtenues à partir du bilan massique d'un radiotraceur présent partout sur la Terre et transmis par voie trophique ( 137 Cs) pour déterminer la perturbation bioénergétique de la perchaude (Perca flavescens) de lacs pollués par des métaux lourds (Cd, Cu et Zn). L'incrément annuel de croissance en rapport avec le bilan énergétique global (efficacité de conversion) était environ 3 fois plus bas chez les perchaudes des lacs pollués par les métaux, dont le mé-tabolisme du cortisol était perturbé, que chez les perchaudes de lacs témoins (4,2% comparativement à 10,8%), ce qui indique que les coûts énergétiques totaux sont plus élevés chez les poissons exposés aux polluants. De plus, dans les lacs pollués par les métaux, les populations de perchaudes adultes étaient dominantes et la diversité des proies était faible, ce qui laisse penser que les effets des polluants se font sentir à divers niveaux du biote. Notre approche bioéner-gétique in situ en matière d'évaluation de la toxicité offre un paramètre mesurable et écologiquement pertinent pour évaluer les effets sublétaux des polluants sur les communautés de poissons.[Traduit par la Rédaction] Sherwood et al. 450

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“…Given this rapidity of Cd exchange, insects would likely be useful for monitoring Cd within weeks of a change in exposure concentrations. In contrast, the Cd concentrations in some bivalves are reported to take years to attain those of indigenous animals (Couillard et al 1995;Baudrimont et al 2003).…”

mentioning

confidence: 98%

Exchange rates of cadmium between a burrowing mayfly and its surroundings in nature

Michaud¹,

Hare²,

Campbell³

2005

Limnol. Oceanogr.

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We conducted a reciprocal transfer of nymphs of the burrowing mayfly Hexagenia limbata between two rivers harboring populations of this insect that differed in their cadmium (Cd) concentrations. We measured Cd uptake and loss rates simultaneously in the field at several levels of biological organization: in the gut and body of whole nymphs as well as in three subcellular fractions. The most marked changes in Cd concentrations occurred in the insect's gut, where Cd was largely associated with heat-stable proteins, a fraction that includes the metal-binding protein metallothionein. Because most of the Cd that entered nymphs was detoxified in this manner, we would not expect it to have direct toxic effects; however, it would be readily transferred to higher trophic levels. Our data on subcellular partitioning suggest that there is a fast-exchanging pool of Cd bound to heat-stable proteins such as metallothionein and a slowexchanging pool bound to either or both of the remaining fractions (heat-denatured proteins; remainder, which includes exoskeleton and granules). Nymphs of H. limbata responded rapidly to changes in bioavailable Cd, which suggests that they would be useful for monitoring changes in ambient Cd for weeks or months in the field. A one-compartment model successfully explained the changes we observed in nymph Cd values. Furthermore, values of the model constant for Cd loss were close to those reported for Hexagenia rigida in the laboratory, which suggests that accumulation patterns observed in the laboratory can be representative of those measured in nature.

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Field transplantation of a freshwater bivalve, Pyganodon grandis, across a metal contamination gradient. I. Temporal changes in metallothionein and metal (Cd, Cu, and Zn) concentrations in soft tissues

Cited by 69 publications

References 17 publications

Oxidative damage effects in the copepod Tigriopus japonicus Mori experimentally exposed to nickel

Oxidative damage effects in the copepod Tigriopus japonicus Mori experimentally exposed to nickel

Bioenergetic costs of heavy metal exposure in yellow perch (Perca flavescens): in situ estimates with a radiotracer (¹³⁷Cs) technique

Exchange rates of cadmium between a burrowing mayfly and its surroundings in nature

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Field transplantation of a freshwater bivalve, Pyganodon grandis, across a metal contamination gradient. I. Temporal changes in metallothionein and metal (Cd, Cu, and Zn) concentrations in soft tissues

Cited by 69 publications

References 17 publications

Oxidative damage effects in the copepod Tigriopus japonicus Mori experimentally exposed to nickel

Oxidative damage effects in the copepod Tigriopus japonicus Mori experimentally exposed to nickel

Bioenergetic costs of heavy metal exposure in yellow perch (Perca flavescens): in situ estimates with a radiotracer (137Cs) technique

Exchange rates of cadmium between a burrowing mayfly and its surroundings in nature

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Bioenergetic costs of heavy metal exposure in yellow perch (Perca flavescens): in situ estimates with a radiotracer (¹³⁷Cs) technique