Filtrating colorectal cancer associated genes by integrated analyses of global DNA methylation and hydroxymethylation in cancer and normal tissue

Li, Ming; Gao, Fei; Xia, Yudong; Zhao, Wei; Jin, Congcong; Luo, Huijuan; Wang, Junwen; Li, Qingshu; Wang, Yalan

doi:10.1038/srep31826

Cited by 21 publications

(13 citation statements)

References 46 publications

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“…Finally, using thymine-DNA glycosylase (TDG), the carboxyl group from 5formylcytosine and 5-carboxylcytosine are removed to restore unmethylated cytosine pool (Figure 1; Silva et al, 2017;Jayanthi et al, 2018;Zhu et al, 2018). 5-hmC is an intermediate throughout the process (Li et al, 2016).…”

Section: Mechanism Of Dna Hydroxymethylationmentioning

confidence: 99%

DNA Methylation and Hydroxymethylation in Cervical Cancer: Diagnosis, Prognosis and Treatment

Zhu

Tian

et al. 2020

Front. Genet.

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Recent discoveries have led to the development of novel ideas and techniques that have helped elucidate the correlation between epigenetics and tumor biology. Nowadays, the field of tumor genetics has evolved to include a new type of regulation by epigenetics. An increasing number of studies have demonstrated the importance of DNA methylation and hydroxymethylation in specific genes in the progression of cervical cancer. Determining the methylation and hydroxymethylation profiles of these genes will help in the early prevention and diagnosis, monitoring recurrence, prognosis, and treatment of patients with cervical cancer. In this review, we focus on the significance of aberrant DNA methylation and hydroxymethylation in cervical cancer and the use of these epigenetic signatures in clinical settings.

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Section: Mechanism Of Dna Hydroxymethylationmentioning

confidence: 99%

DNA Methylation and Hydroxymethylation in Cervical Cancer: Diagnosis, Prognosis and Treatment

Zhu

Tian

et al. 2020

Front. Genet.

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“…As a bridge between the genetic and environmental factors, epigenetic modification plays an important role in cancer initiation and progression. DNA methylation is acknowledged as the principle mechanism of dysfunction of tumor suppressor genes [ 7 ] and the activation of oncogenes [ 8 ]. DNA methylation has been widely studied in GC and CRC [ 9 – 12 ].…”

Section: Introductionmentioning

confidence: 99%

The role of TFPI2 hypermethylation in the detection of gastric and colorectal cancer

Chen

Wang

et al. 2017

Oncotarget

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Gastrointestinal cancer is a prevalent disease with high morbidity and mortality. Tissue factor pathway inhibitor 2 (TFPI2) gene could protect the extracellular matrix of cancer cells from degradation and tumor invasion. The goal of our study was to estimate the diagnostic value of TFPI2 hypermethylation in gastric cancer (GC) and colorectal cancer (CRC). TFPI2 methylation was measured by quantitative methylation-specific polymerase chain reaction (qMSP) method in 114 GC and 80 CRC tissues and their paired non-tumor tissues. Our results showed that TFPI2 methylation was significantly higher in tumor tissues (GC: 29.940% vs. 12.785%, P < 0.001; CRC: 26.930% vs. 5.420%, P < 0.001). The methylation level of TFPI2 in colorectal tumor tissues was significantly higher than that in colorectal normal tissues (26.930% versus 0.002%, P < 0.00001). In GC, TFPI2 hypermethylation yielded an area under the curve (AUC) of 0.762 (95% CI: 0.696–0.828) with a sensitivity of 68% and a specificity of 83%. In CRC, TFPI2 hypermethylation yielded an AUC of 0.759 (95% CI: 0.685–0.834) with a sensitivity of 61% and a specificity of 84%. Similarly, TCGA data also supported TFPI2 hypermethylation was a promising diagnostic marker for GC and CRC. Moreover, the dual-luciferase reporter assay showed TFPI2 fragment could upregulate gene expression (fold change = 5, P = 0.005). Data mining further indicated that TFPI2 expression in CRC cell lines was significantly increased after 5’-AZA-deoxycytidine treatment (fold change > 1.37). In conclusion, TFPI2 hypermethylation might be a promising diagnostic biomarker for GC and CRC.

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“…In addition, HBB expression has been found to be inhibited in anaplastic thyroid cancer cell lines/tissues and oral tongue squamous cell carcinoma (Onda et al, ; Suresh et al, ). As a Kunitz serine protease inhibitor, TFPI2 can inhibit many serine proteases, including trypsin, plasmin, plasma kallikrein, and chymotrypsin (Li et al, ). Studies have shown that the low expression of TFPI2 in small cell lung cancer (SCLC) can promote proliferation of the cells (Cao, Guo, Yin, Li, & Zhou, ).…”

Section: Discussionmentioning

confidence: 99%

Biomarker expression analysis in different age groups revealed age was a risk factor for breast cancer

Liu

et al. 2019

Journal Cellular Physiology

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The relationship between age and breast cancer is ambiguous. Here, we analyzed the differential expression pattern of long noncoding RNAs (lncRNAs) and messenger RNAs (mRNAs) in different age groups to provide an effective association between age and breast cancer risk at the molecular level. We integrated the microarray information from the Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) data sets. The patients were divided into young ( < 50 years) and old ( ≥ 50 years) age groups and evaluated by differential gene expression, weighted gene correlation network analysis (WGCNA), functional enrichment analyses, and coexpression analysis. To determine their potential clinical significance, univariate Cox regression analysis and survival assessment were conducted. We identified two lncRNAs (AL139280.1 and AP000851.1) and three mRNAs (MT1M, HBB, and TFPI2) as the risk markers, and Gene set enrichment analysis (GSEA) focusing on a single gene revealed that "pyrimidine metabolism," "cell cycle," and "P53 signaling pathway"were coenriched. These data demonstrated that age may be a risk factor for breast carcinogenesis and prognosis and provide an in-depth molecular characterization based on the expression patterns of lncRNAs and mRNAs. K E Y W O R D Sage, breast cancer, GEO, lncRNA-mRNA, TCGA

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Filtrating colorectal cancer associated genes by integrated analyses of global DNA methylation and hydroxymethylation in cancer and normal tissue

Cited by 21 publications

References 46 publications

DNA Methylation and Hydroxymethylation in Cervical Cancer: Diagnosis, Prognosis and Treatment

DNA Methylation and Hydroxymethylation in Cervical Cancer: Diagnosis, Prognosis and Treatment

The role of TFPI2 hypermethylation in the detection of gastric and colorectal cancer

Biomarker expression analysis in different age groups revealed age was a risk factor for breast cancer

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