Fine structure of the terminal organ of the house fly larva, Musca domestica L.

Chu‐Wang, I‐Wu; Axtell, R. C.

doi:10.1007/bf00306874

Cited by 76 publications

(46 citation statements)

References 19 publications

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“…The electrophysiological responses obtained here following stimulation of the dorsal organ with banana volatiles confirms the olfactory function assigned to it based on ultrastructure studies on Musca (Chu and Axtell, 1971;Chu-Wang and Axtell, 1972a), Drosophila (Singh and Singh, 1984) and Hylemya (Honda and Ishikawa, 1987a) larvae. The multicellular response recorded from the dorsal organ to banana odour is clearly different from that evoked by single constituents of the odour, for whereas the former evoked responses of cells of very different amplitudes, the response to hexanol or trans-2-hexen-1-ol for example is characterized by modulation of the frequency of just one unit.…”

Section: Dorsal Organ Electrophysiologysupporting

confidence: 82%

Neurophysiological and behavioural evidence for an olfactory function for the dorsal organ and a gustatory one for the terminal organ in Drosophila melanogaster larvae

Oppliger

Guérin

Vlimant

2000

Journal of Insect Physiology

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Multicellular electrophysiological responses from the dorsal organ on the cephalic lobes of third instar Drosophila melanogaster larvae (wild-type Canton S) stimulated with a cold-trapped banana volatile extract showed that this structure has an olfactory function in the fruit fly. Responses of the dorsal organ were also recorded to constituents of the banana volatile extract as they eluted from a gas chromatographic column (GC-coupled dorsal organ electrophysiology). The active chemostimulants were identified as 2-heptanone, isoamyl alcohol, hexyl acetate, hexanol and hexyl butyrate by gas chromatography-coupled mass spectrometry. Applying the same recording system to the terminal organ sensilla, no responses were obtained to either the banana volatile bouquet or its constituents. By contrast, high frequency multicellular responses were recorded in response to touching the terminal organ with the gustatory stimuli KCl and grapefruit juice; responses were absent on similar stimulation of the dorsal organ with either NaCl or KCl. This suggests a role for olfaction by the dorsal organ and for gustation by the terminal organ in Drosophila larvae.In a 7-mm high wind tunnel with a thin 1.2% agar floor, the Drosophila larvae showed odour-conditioned upwind responses in an air stream of 0.1 m/s bearing banana volatiles. Drosophila larvae responded best to the odour of cut bananas. A 1:1 mixture of the banana odour constituents 2-heptanone and hexanol (at either 50 or 100 µg source dose each) proved as attractive as the known larval attractants propionic acid and isoamyl acetate on their own at 100 µg, whereas hexanol and 2-heptanone on their own at a 100 µg source dose were less attractive. The stronger behavioural response to the banana volatile bouquet and to the binary mixture serves to underline the multireceptor nature of the dorsal organ response to food odour in Drosophila.

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Section: Dorsal Organ Electrophysiologysupporting

confidence: 82%

Neurophysiological and behavioural evidence for an olfactory function for the dorsal organ and a gustatory one for the terminal organ in Drosophila melanogaster larvae

Oppliger

Guérin

Vlimant

2000

Journal of Insect Physiology

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“…Moreover, in contrast to eye or antennal development, DO formation is not embedded in a 'sensory field' that comprises hundreds of similar, closelyspaced elements, such as ommatidia or antennal sensilla. Rather, the DO is a unique, complex organ, which in Musca includes at least six morphologically distinct types of sensilla (Chu and Axtell 1971;Chu-Wang and Axtell 1972). The formation of such an intricate organ would be hard to explain based on cell recruitment.…”

mentioning

confidence: 99%

Role of proneural genes in the formation of the larval olfactory organ of Drosophila

et al. 2007

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In this paper, we address the role of proneural genes in the formation of the dorsal organ in the Drosophila larva. This organ is an intricate compound comprising the multineuronal dome-the exclusive larval olfactory organand a number of mostly gustatory sensilla. We first determine the numbers of neurons and of the different types of accessory cells in the dorsal organ. From these data, we conclude that the dorsal organ derives from 14 sensory organ precursor cells. Seven of them appear to give rise to the dome, which therefore may be composed of seven fused sensilla, whereas the other precursors produce the remaining sensilla of the dorsal organ. By a loss-of-function approach, we then analyze the role of atonal, amos, and the achaetescute complex (AS-C), which in the adult are the exclusive proneural genes required for chemosensory organ specification. We show that atonal and amos are necessary and sufficient in a complementary way for four and three of the sensory organ precursors of the dome, respectively. AS-C, on the other hand, is implicated in specifying the non-olfactory sensilla, partially in cooperation with atonal and/or amos. Similar links for these proneural genes with olfactory and gustatory function have been established in the adult fly.However, such conserved gene function is not trivial, given that adult and larval chemosensory organs are anatomically very different and that the development of adult olfactory sensilla involves cell recruitment, which is unlikely to play a role in dome formation.

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“…The cellular relationships of the ch organs of Drosophila revealed by fluorescent dye staining correspond well with the detailed morphology revealed by ultrastructural studies of this type of sense organ ( Fig. 1) (Drosophila : Hartenstein, 1988;Shanbhag et al, 1992;Uga and Kuwabara, 1965;other Diptera: Boo and Richards, 1975;Chu-Wang and Axtell, 1972b;Hallberg, 1984;Pappas and Larsen, 1976). The ch neurons have a relatively straight dendrite with an abrupt transition between the inner dendritic segment (ids) and the thinner outer segment (ods).…”

Section: Resultsmentioning

confidence: 77%

“…3A,B). Such a structure is common in ch neurons of this type; e.g., in Diptera they are found in antennal and leg ch sensilla of Drosophila (Shanbhag et al, 1992;Uga and Kuwabara, 1965) and the blowfly Calliphora (Hallberg, 1984), the cephalic ch sensilla of the larval housefly (Chu-Wang and Axtell, 1972b), the antennal and labellar sensilla of the mosquito (Boo and Richards, 1975;Pappas and Larsen, 1976), and the tarsal sensilla of the midge, Chironomus (Seifert and Heinzeller, 1989). It is a ch-specific character because it has not been reported in the external type I sense organs of insects.…”

Section: Resultsmentioning

confidence: 98%

See 1 more Smart Citation

Transformation of external sensilla to chordotonal sensilla in thecut mutant ofDrosophila assessed by single-cell marking in the embryo and larva

Merritt

1997

Microsc. Res. Tech.

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Fine structure of the terminal organ of the house fly larva, Musca domestica L.

Cited by 76 publications

References 19 publications

Neurophysiological and behavioural evidence for an olfactory function for the dorsal organ and a gustatory one for the terminal organ in Drosophila melanogaster larvae

Neurophysiological and behavioural evidence for an olfactory function for the dorsal organ and a gustatory one for the terminal organ in Drosophila melanogaster larvae

Role of proneural genes in the formation of the larval olfactory organ of Drosophila

Transformation of external sensilla to chordotonal sensilla in thecut mutant ofDrosophila assessed by single-cell marking in the embryo and larva

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